Restriction map for
pF1KE0087
(Linear) (Six-base) MAPSORT of: KIEE0087.seq Check: 3140 from: 1 to: 2766
KIEE0087.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 403 2447 2766
Size: 245 158 2044 319
Fragments arranged by size:
2044 319 245 158
AflIII A'CryG_T
Cuts at: 0 122 1703 2071 2766
Size: 122 1581 368 695
Fragments arranged by size:
1581 695 368 122
ApoI r'AATT_y
Cuts at: 0 236 484 561 699 936 1366 1637 2766
Size: 236 248 77 138 237 430 271 1129
Fragments arranged by size:
1129 430 271 248 237 236 138 77
BamHI G'GATC_C
Cuts at: 0 214 2766
Size: 214 2552
BanII G_rGCy'C
Cuts at: 0 1791 2766
Size: 1791 975
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 75 409 1424 2093 2647 2718 2766
Size: 36 39 334 1015 669 554 71 48
Fragments arranged by size:
1015 669 554 334 71 48 39 36
BciVI GTATCCnnnnn_n'
Cuts at: 0 1167 2766
Size: 1167 1599
BclI T'GATC_A
Cuts at: 0 2216 2766
Size: 2216 550
BfrBI ATG'CAT
Cuts at: 0 2749 2766
Size: 2749 17
BlpI GC'TnA_GC
Cuts at: 0 1539 2766
Size: 1539 1227
BmgBI CAC'GTC
Cuts at: 0 1706 2766
Size: 1706 1060
BmtI G_CTAG'C
Cuts at: 0 2140 2766
Size: 2140 626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2106 2766
Size: 743 1363 660
Fragments arranged by size:
1363 743 660
BsaI GGTCTCn'nnnn_
Cuts at: 0 837 2766
Size: 837 1929
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 832 862 2766
Size: 91 30 711 30 1904
Fragments arranged by size:
1904 711 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 2379 2766
Size: 112 2267 387
Fragments arranged by size:
2267 387 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 2766
Size: 129 2637
BsiHKAI G_wGCw'C
Cuts at: 0 1165 1540 1791 2247 2766
Size: 1165 375 251 456 519
Fragments arranged by size:
1165 519 456 375 251
BsmI GAATG_Cn'
Cuts at: 0 892 2766
Size: 892 1874
Bsp1286I G_dGCh'C
Cuts at: 0 1165 1540 1791 2247 2766
Size: 1165 375 251 456 519
Fragments arranged by size:
1165 519 456 375 251
BsrDI GCAATG_nn'
Cuts at: 0 317 544 1610 2766
Size: 317 227 1066 1156
Fragments arranged by size:
1156 1066 317 227
BsrGI T'GTAC_A
Cuts at: 0 438 1700 2766
Size: 438 1262 1066
Fragments arranged by size:
1262 1066 438
BstYI r'GATC_y
Cuts at: 0 214 2766
Size: 214 2552
BtsI GCAGTG_nn'
Cuts at: 0 821 2766
Size: 821 1945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 482 517 1424 1459 2766
Size: 482 35 907 35 1307
Fragments arranged by size:
1307 907 482 35 35
DraI TTT'AAA
Cuts at: 0 1374 1515 1695 1965 2535 2766
Size: 1374 141 180 270 570 231
Fragments arranged by size:
1374 570 270 231 180 141
EarI CTCTTCn'nnn_
Cuts at: 0 1796 2766
Size: 1796 970
EcoICRI GAG'CTC
Cuts at: 0 1789 2766
Size: 1789 977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 403 743 2106 2447 2766
Size: 245 158 340 1363 341 319
Fragments arranged by size:
1363 341 340 319 245 158
EcoRI G'AATT_C
Cuts at: 0 1366 2766
Size: 1366 1400
EcoRV GAT'ATC
Cuts at: 0 2045 2172 2183 2766
Size: 2045 127 11 583
Fragments arranged by size:
2045 583 127 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 375 1761 2452 2766
Size: 375 1386 691 314
Fragments arranged by size:
1386 691 375 314
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1285 1317 2766
Size: 832 32 421 32 1449
Fragments arranged by size:
1449 832 421 32 32
HincII GTy'rAC
Cuts at: 0 878 2766
Size: 878 1888
HindIII A'AGCT_T
Cuts at: 0 1508 2766
Size: 1508 1258
MfeI C'AATT_G
Cuts at: 0 868 2207 2766
Size: 868 1339 559
Fragments arranged by size:
1339 868 559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1868 2209 2605 2766
Size: 1165 703 341 396 161
Fragments arranged by size:
1165 703 396 341 161
MslI CAynn'nnrTG
Cuts at: 0 788 1041 1213 2766
Size: 788 253 172 1553
Fragments arranged by size:
1553 788 253 172
NdeI CA'TA_TG
Cuts at: 0 189 2766
Size: 189 2577
NheI G'CTAG_C
Cuts at: 0 2136 2766
Size: 2136 630
NsiI A_TGCA'T
Cuts at: 0 2751 2766
Size: 2751 15
NspI r_CATG'y
Cuts at: 0 126 2445 2493 2766
Size: 126 2319 48 273
Fragments arranged by size:
2319 273 126 48
PasI CC'CwG_GG
Cuts at: 0 66 2766
Size: 66 2700
PciI A'CATG_T
Cuts at: 0 122 2766
Size: 122 2644
PflMI CCAn_nnn'nTGG
Cuts at: 0 857 2766
Size: 857 1909
PfoI T'CCnGG_A
Cuts at: 0 731 2766
Size: 731 2035
PshAI GACnn'nnGTC
Cuts at: 0 829 2209 2766
Size: 829 1380 557
Fragments arranged by size:
1380 829 557
PsiI TTA'TAA
Cuts at: 0 293 976 2766
Size: 293 683 1790
Fragments arranged by size:
1790 683 293
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1319 1351 2766
Size: 1319 32 1415
Fragments arranged by size:
1415 1319 32
SacI G_AGCT'C
Cuts at: 0 1791 2766
Size: 1791 975
SapI GCTCTTCn'nnn_
Cuts at: 0 1796 2766
Size: 1796 970
ScaI AGT'ACT
Cuts at: 0 2451 2766
Size: 2451 315
SfcI C'TryA_G
Cuts at: 0 716 881 1569 2766
Size: 716 165 688 1197
Fragments arranged by size:
1197 716 688 165
SphI G_CATG'C
Cuts at: 0 2445 2493 2766
Size: 2445 48 273
Fragments arranged by size:
2445 273 48
StyI C'CwwG_G
Cuts at: 0 1585 2766
Size: 1585 1181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 998 2766
Size: 514 484 1768
Fragments arranged by size:
1768 514 484
TatI w'GTAC_w
Cuts at: 0 438 1700 2074 2449 2766
Size: 438 1262 374 375 317
Fragments arranged by size:
1262 438 375 374 317
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 91 123 2223 2255 2766
Size: 91 32 2100 32 511
Fragments arranged by size:
2100 511 91 32 32
XbaI T'CTAG_A
Cuts at: 0 1476 2350 2766
Size: 1476 874 416
Fragments arranged by size:
1476 874 416
Enzymes that do cut:
AcuI AflIII ApoI BamHI BanII BbsI BciVI BclI
BfrBI BlpI BmgBI BmtI BpmI BsaI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstYI BtsI
CspCI DraI EarI EcoICRI Eco57MI EcoRI EcoRV FalI
Hin4I HincII HindIII MfeI MmeI MslI NdeI NheI
NsiI NspI PasI PciI PflMI PfoI PshAI PsiI
PsrI SacI SapI ScaI SfcI SphI StyI TaqII
TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BglI BglII Bme1580I BmrI BplI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MscI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI PacI
PmeI PmlI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XcmI XhoI XmaI XmnI
ZraI