Restriction map for
pF1KE0091
(Linear) (Six-base) MAPSORT of: KIEE0091.seq Check: 1072 from: 1 to: 1896
KIEE0091.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 745 761 1896
Size: 745 16 1135
Fragments arranged by size:
1135 745 16
Acc65I G'GTAC_C
Cuts at: 0 1092 1896
Size: 1092 804
AflIII A'CryG_T
Cuts at: 0 215 358 1271 1896
Size: 215 143 913 625
Fragments arranged by size:
913 625 215 143
AgeI A'CCGG_T
Cuts at: 0 1481 1896
Size: 1481 415
AlwNI CAG_nnn'CTG
Cuts at: 0 555 998 1896
Size: 555 443 898
Fragments arranged by size:
898 555 443
ApoI r'AATT_y
Cuts at: 0 299 1896
Size: 299 1597
AvaI C'yCGr_G
Cuts at: 0 826 1741 1896
Size: 826 915 155
Fragments arranged by size:
915 826 155
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 708 741 1896
Size: 708 33 1155
Fragments arranged by size:
1155 708 33
BanI G'GyrC_C
Cuts at: 0 66 99 1027 1092 1896
Size: 66 33 928 65 804
Fragments arranged by size:
928 804 66 65 33
BanII G_rGCy'C
Cuts at: 0 1810 1896
Size: 1810 86
BbeI G_GCGC'C
Cuts at: 0 70 103 1896
Size: 70 33 1793
Fragments arranged by size:
1793 70 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1508 1729 1896
Size: 1508 221 167
Fragments arranged by size:
1508 221 167
BbvCI CC'TCA_GC
Cuts at: 0 44 1103 1896
Size: 44 1059 793
Fragments arranged by size:
1059 793 44
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1017 1051 1440 1474 1896
Size: 1017 34 389 34 422
Fragments arranged by size:
1017 422 389 34 34
BclI T'GATC_A
Cuts at: 0 1550 1595 1896
Size: 1550 45 301
Fragments arranged by size:
1550 301 45
BglII A'GATC_T
Cuts at: 0 914 1776 1896
Size: 914 862 120
Fragments arranged by size:
914 862 120
BlpI GC'TnA_GC
Cuts at: 0 181 1896
Size: 181 1715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1735 1896
Size: 337 1398 161
Fragments arranged by size:
1398 337 161
Bpu10I CC'TnA_GC
Cuts at: 0 44 472 1103 1896
Size: 44 428 631 793
Fragments arranged by size:
793 631 428 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1597 1831 1896
Size: 1597 234 65
Fragments arranged by size:
1597 234 65
BsaAI yAC'GTr
Cuts at: 0 276 359 765 1896
Size: 276 83 406 1131
Fragments arranged by size:
1131 406 276 83
BsaBI GATnn'nnATC
Cuts at: 0 1207 1582 1708 1896
Size: 1207 375 126 188
Fragments arranged by size:
1207 375 188 126
BsaHI Gr'CG_yC
Cuts at: 0 67 100 1896
Size: 67 33 1796
Fragments arranged by size:
1796 67 33
BsaWI w'CCGG_w
Cuts at: 0 1289 1481 1896
Size: 1289 192 415
Fragments arranged by size:
1289 415 192
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 266 1896
Size: 61 205 1630
Fragments arranged by size:
1630 205 61
BseYI C'CCAG_C
Cuts at: 0 207 674 1042 1463 1638 1768 1896
Size: 207 467 368 421 175 130 128
Fragments arranged by size:
467 421 368 207 175 130 128
BsiHKAI G_wGCw'C
Cuts at: 0 626 659 1896
Size: 626 33 1237
Fragments arranged by size:
1237 626 33
BsiWI C'GTAC_G
Cuts at: 0 1079 1896
Size: 1079 817
BsmI GAATG_Cn'
Cuts at: 0 259 597 1896
Size: 259 338 1299
Fragments arranged by size:
1299 338 259
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1879 1896
Size: 1879 17
Bsp1286I G_dGCh'C
Cuts at: 0 626 659 1810 1896
Size: 626 33 1151 86
Fragments arranged by size:
1151 626 86 33
BspEI T'CCGG_A
Cuts at: 0 1289 1896
Size: 1289 607
BspHI T'CATG_A
Cuts at: 0 1199 1553 1896
Size: 1199 354 343
Fragments arranged by size:
1199 354 343
BsrBI CCG'CTC
Cuts at: 0 955 1698 1896
Size: 955 743 198
Fragments arranged by size:
955 743 198
BsrDI GCAATG_nn'
Cuts at: 0 36 1097 1896
Size: 36 1061 799
Fragments arranged by size:
1061 799 36
BsrFI r'CCGG_y
Cuts at: 0 1301 1481 1517 1896
Size: 1301 180 36 379
Fragments arranged by size:
1301 379 180 36
BsrGI T'GTAC_A
Cuts at: 0 1379 1896
Size: 1379 517
BssHII G'CGCG_C
Cuts at: 0 106 1896
Size: 106 1790
BssSI C'ACGA_G
Cuts at: 0 142 1354 1896
Size: 142 1212 542
Fragments arranged by size:
1212 542 142
BstEII G'GTnAC_C
Cuts at: 0 1155 1254 1297 1896
Size: 1155 99 43 599
Fragments arranged by size:
1155 599 99 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 939 1038 1896
Size: 939 99 858
Fragments arranged by size:
939 858 99
BstYI r'GATC_y
Cuts at: 0 914 1565 1577 1703 1776 1896
Size: 914 651 12 126 73 120
Fragments arranged by size:
914 651 126 120 73 12
BstZ17I GTA'TAC
Cuts at: 0 762 1896
Size: 762 1134
BtgI C'CryG_G
Cuts at: 0 1262 1810 1896
Size: 1262 548 86
Fragments arranged by size:
1262 548 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1848 1896
Size: 1848 48
BtsI GCAGTG_nn'
Cuts at: 0 1735 1896
Size: 1735 161
DraIII CAC_nnn'GTG
Cuts at: 0 1718 1896
Size: 1718 178
DrdI GACnn_nn'nnGTC
Cuts at: 0 136 889 1896
Size: 136 753 1007
Fragments arranged by size:
1007 753 136
EaeI y'GGCC_r
Cuts at: 0 150 188 635 1436 1519 1896
Size: 150 38 447 801 83 377
Fragments arranged by size:
801 447 377 150 83 38
EarI CTCTTCn'nnn_
Cuts at: 0 1435 1896
Size: 1435 461
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1896
Size: 22 1874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1735 1896
Size: 337 1398 161
Fragments arranged by size:
1398 337 161
EcoO109I rG'GnC_Cy
Cuts at: 0 630 830 1120 1896
Size: 630 200 290 776
Fragments arranged by size:
776 630 290 200
EcoRI G'AATT_C
Cuts at: 0 299 1896
Size: 299 1597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 870 1896
Size: 870 1026
HaeII r_GCGC'y
Cuts at: 0 19 70 103 225 1896
Size: 19 51 33 122 1671
Fragments arranged by size:
1671 122 51 33 19
HincII GTy'rAC
Cuts at: 0 1364 1760 1896
Size: 1364 396 136
Fragments arranged by size:
1364 396 136
HindIII A'AGCT_T
Cuts at: 0 1795 1896
Size: 1795 101
HpaI GTT'AAC
Cuts at: 0 1364 1896
Size: 1364 532
KasI G'GCGC_C
Cuts at: 0 66 99 1896
Size: 66 33 1797
Fragments arranged by size:
1797 66 33
KpnI G_GTAC'C
Cuts at: 0 1096 1896
Size: 1096 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 905 1896
Size: 343 562 991
Fragments arranged by size:
991 562 343
MscI TGG'CCA
Cuts at: 0 637 1438 1896
Size: 637 801 458
Fragments arranged by size:
801 637 458
MslI CAynn'nnrTG
Cuts at: 0 428 1162 1714 1896
Size: 428 734 552 182
Fragments arranged by size:
734 552 428 182
MspA1I CmG'CkG
Cuts at: 0 89 1025 1179 1896
Size: 89 936 154 717
Fragments arranged by size:
936 717 154 89
NarI GG'CG_CC
Cuts at: 0 67 100 1896
Size: 67 33 1796
Fragments arranged by size:
1796 67 33
NspI r_CATG'y
Cuts at: 0 1275 1380 1896
Size: 1275 105 516
Fragments arranged by size:
1275 516 105
PasI CC'CwG_GG
Cuts at: 0 24 500 1561 1896
Size: 24 476 1061 335
Fragments arranged by size:
1061 476 335 24
PciI A'CATG_T
Cuts at: 0 1271 1896
Size: 1271 625
PfoI T'CCnGG_A
Cuts at: 0 314 1896
Size: 314 1582
PpuMI rG'GwC_Cy
Cuts at: 0 630 1896
Size: 630 1266
PsiI TTA'TAA
Cuts at: 0 968 977 1896
Size: 968 9 919
Fragments arranged by size:
968 919 9
PstI C_TGCA'G
Cuts at: 0 85 590 1896
Size: 85 505 1306
Fragments arranged by size:
1306 505 85
SfcI C'TryA_G
Cuts at: 0 81 570 586 746 990 1896
Size: 81 489 16 160 244 906
Fragments arranged by size:
906 489 244 160 81 16
SfoI GGC'GCC
Cuts at: 0 68 101 1896
Size: 68 33 1795
Fragments arranged by size:
1795 68 33
SmaI CCC'GGG
Cuts at: 0 828 1896
Size: 828 1068
SmlI C'TyrA_G
Cuts at: 0 1612 1846 1896
Size: 1612 234 50
Fragments arranged by size:
1612 234 50
StyI C'CwwG_G
Cuts at: 0 1148 1280 1896
Size: 1148 132 616
Fragments arranged by size:
1148 616 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1455 1896
Size: 1455 441
TatI w'GTAC_w
Cuts at: 0 434 1379 1601 1616 1896
Size: 434 945 222 15 280
Fragments arranged by size:
945 434 280 222 15
Tth111I GACn'n_nGTC
Cuts at: 0 741 1896
Size: 741 1155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1104 1896
Size: 1104 792
XmaI C'CCGG_G
Cuts at: 0 826 1896
Size: 826 1070
Enzymes that do cut:
AccI Acc65I AflIII AgeI AlwNI ApoI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglII
BlpI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII
HincII HindIII HpaI KasI KpnI MmeI MscI MslI
MspA1I NarI NspI PasI PciI PfoI PpuMI PsiI
PstI SfcI SfoI SmaI SmlI StyI TaqII TatI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BciVI BfrBI BglI Bme1580I BmgBI BmrI BmtI
BplI BsaI BsaXI BsgI BsiEI BspMI BstAPI BstBI
Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI EcoRV
FseI FspI FspAI Hin4I MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI XbaI XhoI XmnI
ZraI