Restriction map for
pF1KE0095
(Linear) (Six-base) MAPSORT of: KIEE0095.seq Check: 8277 from: 1 to: 3783
KIEE0095.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3009 3783
Size: 3009 774
AatII G_ACGT'C
Cuts at: 0 1751 3783
Size: 1751 2032
AccI GT'mk_AC
Cuts at: 0 2270 3783
Size: 2270 1513
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 1147 1601 2214 3783
Size: 267 880 454 613 1569
Fragments arranged by size:
1569 880 613 454 267
AfeI AGC'GCT
Cuts at: 0 694 3783
Size: 694 3089
AflIII A'CryG_T
Cuts at: 0 2555 2630 3668 3692 3783
Size: 2555 75 1038 24 91
Fragments arranged by size:
2555 1038 91 75 24
AgeI A'CCGG_T
Cuts at: 0 3634 3783
Size: 3634 149
AhdI GACnn_n'nnGTC
Cuts at: 0 1752 3783
Size: 1752 2031
AleI CACnn'nnGTG
Cuts at: 0 1607 1715 2051 2344 2530 3783
Size: 1607 108 336 293 186 1253
Fragments arranged by size:
1607 1253 336 293 186 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 65 3783
Size: 65 3718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 3783
Size: 1351 32 2400
Fragments arranged by size:
2400 1351 32
AlwNI CAG_nnn'CTG
Cuts at: 0 204 215 1122 1467 2699 3681 3783
Size: 204 11 907 345 1232 982 102
Fragments arranged by size:
1232 982 907 345 204 102 11
ApaI G_GGCC'C
Cuts at: 0 596 3773 3783
Size: 596 3177 10
Fragments arranged by size:
3177 596 10
ApaLI G'TGCA_C
Cuts at: 0 1452 3783
Size: 1452 2331
ApoI r'AATT_y
Cuts at: 0 1691 3783
Size: 1691 2092
AvaI C'yCGr_G
Cuts at: 0 27 65 136 169 383 576 582 1232
Size: 27 38 71 33 214 193 6 650
Cuts at: 1232 1569 2719 3783
Size: 337 1150 1064
Fragments arranged by size:
1150 1064 650 337 214 193 71 38
33 27 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3623 3656 3783
Size: 3623 33 127
Fragments arranged by size:
3623 127 33
BanI G'GyrC_C
Cuts at: 0 54 60 387 526 616 2284 2844 3783
Size: 54 6 327 139 90 1668 560 939
Fragments arranged by size:
1668 939 560 327 139 90 54 6
BanII G_rGCy'C
Cuts at: 0 25 145 583 596 1490 2822 3185 3536
Size: 25 120 438 13 894 1332 363 351
Cuts at: 3536 3773 3783
Size: 237 10
Fragments arranged by size:
1332 894 438 363 351 237 120 25
13 10
BbeI G_GCGC'C
Cuts at: 0 58 64 391 530 620 3783
Size: 58 6 327 139 90 3163
Fragments arranged by size:
3163 327 139 90 58 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 52 2102 2304 3783
Size: 52 2050 202 1479
Fragments arranged by size:
2050 1479 202 52
BbvCI CC'TCA_GC
Cuts at: 0 1199 3783
Size: 1199 2584
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1152 1186 1694 1728 3783
Size: 1152 34 508 34 2055
Fragments arranged by size:
2055 1152 508 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 29 820 2293 3783
Size: 29 791 1473 1490
Fragments arranged by size:
1490 1473 791 29
BlpI GC'TnA_GC
Cuts at: 0 247 1054 2027 2368 2746 3783
Size: 247 807 973 341 378 1037
Fragments arranged by size:
1037 973 807 378 341 247
Bme1580I G_kGCm'C
Cuts at: 0 596 1456 2006 2351 2932 3573 3773 3783
Size: 596 860 550 345 581 641 200 10
Fragments arranged by size:
860 641 596 581 550 345 200 10
BmgBI CAC'GTC
Cuts at: 0 2208 3141 3783
Size: 2208 933 642
Fragments arranged by size:
2208 933 642
BmrI ACTGGGnnnn_n'
Cuts at: 0 1443 2028 2919 3783
Size: 1443 585 891 864
Fragments arranged by size:
1443 891 864 585
BmtI G_CTAG'C
Cuts at: 0 1774 3783
Size: 1774 2009
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 3783
Size: 1659 2124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 538 697 781 2454 2556 3280 3376
Size: 499 39 159 84 1673 102 724 96
Cuts at: 3376 3724 3783
Size: 348 59
Fragments arranged by size:
1673 724 499 348 159 102 96 84
59 39
Bpu10I CC'TnA_GC
Cuts at: 0 1199 2933 3783
Size: 1199 1734 850
Fragments arranged by size:
1734 1199 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1879 3783
Size: 1879 1904
BsaI GGTCTCn'nnnn_
Cuts at: 0 1209 1222 1732 1906 3783
Size: 1209 13 510 174 1877
Fragments arranged by size:
1877 1209 510 174 13
BsaAI yAC'GTr
Cuts at: 0 1534 2564 2640 2829 3695 3783
Size: 1534 1030 76 189 866 88
Fragments arranged by size:
1534 1030 866 189 88 76
BsaBI GATnn'nnATC
Cuts at: 0 844 1381 2341 3526 3783
Size: 844 537 960 1185 257
Fragments arranged by size:
1185 960 844 537 257
BsaHI Gr'CG_yC
Cuts at: 0 55 61 388 527 617 1444 1748 2174
Size: 55 6 327 139 90 827 304 426
Cuts at: 2174 2968 3783
Size: 794 815
Fragments arranged by size:
827 815 794 426 327 304 139 90
55 6
BsaWI w'CCGG_w
Cuts at: 0 3634 3783
Size: 3634 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 2905 2935 3783
Size: 670 30 2205 30 848
Fragments arranged by size:
2205 848 670 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 496 3085 3094 3103 3783
Size: 496 2589 9 9 680
Fragments arranged by size:
2589 680 496 9 9
BseYI C'CCAG_C
Cuts at: 0 2583 3014 3783
Size: 2583 431 769
Fragments arranged by size:
2583 769 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1146 1167 1442 1917 2992 3103 3783
Size: 1146 21 275 475 1075 111 680
Fragments arranged by size:
1146 1075 680 475 275 111 21
BsiEI CG_ry'CG
Cuts at: 0 336 509 827 2277 2654 3648 3783
Size: 336 173 318 1450 377 994 135
Fragments arranged by size:
1450 994 377 336 318 173 135
BsiHKAI G_wGCw'C
Cuts at: 0 752 1055 1067 1456 1490 1904 2207 2606
Size: 752 303 12 389 34 414 303 399
Cuts at: 2606 2804 2822 2834 3185 3503 3783
Size: 198 18 12 351 318 280
Fragments arranged by size:
752 414 399 389 351 318 303 303
280 198 34 18 12 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1572 3783
Size: 1572 2211
Bsp1286I G_dGCh'C
Cuts at: 0 25 145 583 596 752 1055 1067 1456
Size: 25 120 438 13 156 303 12 389
Cuts at: 1456 1490 1904 2006 2207 2351 2606 2804 2822
Size: 34 414 102 201 144 255 198 18
Cuts at: 2822 2834 2932 3185 3503 3536 3573 3773 3783
Size: 12 98 253 318 33 37 200 10
Fragments arranged by size:
438 414 389 318 303 255 253 201
200 198 156 144 120 102 98 37
34 33 25 18 13 12 12 10
BspHI T'CATG_A
Cuts at: 0 1472 3783
Size: 1472 2311
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2256 2588 2885 3009 3014 3783
Size: 2256 332 297 124 5 769
Fragments arranged by size:
2256 769 332 297 124 5
BsrBI CCG'CTC
Cuts at: 0 86 251 293 486 650 681 795 3148
Size: 86 165 42 193 164 31 114 2353
Cuts at: 3148 3168 3777 3783
Size: 20 609 6
Fragments arranged by size:
2353 609 193 165 164 114 86 42
31 20 6
BsrDI GCAATG_nn'
Cuts at: 0 3180 3783
Size: 3180 603
BsrFI r'CCGG_y
Cuts at: 0 57 347 523 3107 3634 3783
Size: 57 290 176 2584 527 149
Fragments arranged by size:
2584 527 290 176 149 57
BssHII G'CGCG_C
Cuts at: 0 501 3783
Size: 501 3282
BssSI C'ACGA_G
Cuts at: 0 751 3783
Size: 751 3032
BstEII G'GTnAC_C
Cuts at: 0 180 2883 3657 3783
Size: 180 2703 774 126
Fragments arranged by size:
2703 774 180 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 735 1173 1647 1782 2018 2053 2529 3141
Size: 735 438 474 135 236 35 476 612
Cuts at: 3141 3624 3783
Size: 483 159
Fragments arranged by size:
735 612 483 476 474 438 236 159
135 35
BstYI r'GATC_y
Cuts at: 0 1541 3783
Size: 1541 2242
Bsu36I CC'TnA_GG
Cuts at: 0 2135 3783
Size: 2135 1648
BtgI C'CryG_G
Cuts at: 0 150 920 1322 3548 3783
Size: 150 770 402 2226 235
Fragments arranged by size:
2226 770 402 235 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 900 1074 2687 3213 3294 3783
Size: 900 174 1613 526 81 489
Fragments arranged by size:
1613 900 526 489 174 81
BtsI GCAGTG_nn'
Cuts at: 0 1055 1454 1602 1710 2455 3029 3783
Size: 1055 399 148 108 745 574 754
Fragments arranged by size:
1055 754 745 574 399 148 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2930 2965 3783
Size: 2930 35 818
Fragments arranged by size:
2930 818 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2881 3783
Size: 2881 902
EaeI y'GGCC_r
Cuts at: 0 206 266 365 506 737 812 824 929
Size: 206 60 99 141 231 75 12 105
Cuts at: 929 1085 1511 2651 2810 3143 3452 3554 3614
Size: 156 426 1140 159 333 309 102 60
Cuts at: 3614 3783
Size: 169
Fragments arranged by size:
1140 426 333 309 231 206 169 159
156 141 105 102 99 75 60 60
12
EagI C'GGCC_G
Cuts at: 0 506 824 2651 3783
Size: 506 318 1827 1132
Fragments arranged by size:
1827 1132 506 318
EarI CTCTTCn'nnn_
Cuts at: 0 1495 1946 2323 2449 2866 3783
Size: 1495 451 377 126 417 917
Fragments arranged by size:
1495 917 451 417 377 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 291 586 694 1062 2659 3783
Size: 291 295 108 368 1597 1124
Fragments arranged by size:
1597 1124 368 295 291 108
EcoICRI GAG'CTC
Cuts at: 0 1488 2820 3183 3783
Size: 1488 1332 363 600
Fragments arranged by size:
1488 1332 600 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 499 538 697 781 1147 1601 2214
Size: 267 232 39 159 84 366 454 613
Cuts at: 2214 2454 2556 3280 3376 3724 3783
Size: 240 102 724 96 348 59
Fragments arranged by size:
724 613 454 366 348 267 240 232
159 102 96 84 59 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3133 3783
Size: 3133 650
EcoO109I rG'GnC_Cy
Cuts at: 0 73 592 2577 2918 3769 3770 3783
Size: 73 519 1985 341 851 1 13
Fragments arranged by size:
1985 851 519 341 73 13 1
EcoRI G'AATT_C
Cuts at: 0 1691 3783
Size: 1691 2092
FspI TGC'GCA
Cuts at: 0 1036 1099 3783
Size: 1036 63 2684
Fragments arranged by size:
2684 1036 63
FspAI rTGC'GCAy
Cuts at: 0 1036 3783
Size: 1036 2747
HaeII r_GCGC'y
Cuts at: 0 58 64 204 391 530 620 696 974
Size: 58 6 140 187 139 90 76 278
Cuts at: 974 2034 3783
Size: 1060 1749
Fragments arranged by size:
1749 1060 278 187 140 139 90 76
58 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1226 1258 1738 1770 3783
Size: 565 32 629 32 480 32 2013
Fragments arranged by size:
2013 629 565 480 32 32 32
HincII GTy'rAC
Cuts at: 0 2553 2628 2658 3783
Size: 2553 75 30 1125
Fragments arranged by size:
2553 1125 75 30
KasI G'GCGC_C
Cuts at: 0 54 60 387 526 616 3783
Size: 54 6 327 139 90 3167
Fragments arranged by size:
3167 327 139 90 54 6
MluI A'CGCG_T
Cuts at: 0 2630 3783
Size: 2630 1153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 3783
Size: 647 3136
MscI TGG'CCA
Cuts at: 0 208 1087 2812 3616 3783
Size: 208 879 1725 804 167
Fragments arranged by size:
1725 879 804 208 167
MslI CAynn'nnrTG
Cuts at: 0 733 739 853 919 1327 1607 1627 1684
Size: 733 6 114 66 408 280 20 57
Cuts at: 1684 1715 2051 2344 2362 2530 2827 3385 3667
Size: 31 336 293 18 168 297 558 282
Cuts at: 3667 3783
Size: 116
Fragments arranged by size:
733 558 408 336 297 293 282 280
168 116 114 66 57 31 20 18
6
MspA1I CmG'CkG
Cuts at: 0 152 215 246 354 371 801 1058 1266
Size: 152 63 31 108 17 430 257 208
Cuts at: 1266 1700 2783 3354 3702 3783
Size: 434 1083 571 348 81
Fragments arranged by size:
1083 571 434 430 348 257 208 152
108 81 63 31 17
NaeI GCC'GGC
Cuts at: 0 59 349 525 3783
Size: 59 290 176 3258
Fragments arranged by size:
3258 290 176 59
NarI GG'CG_CC
Cuts at: 0 55 61 388 527 617 3783
Size: 55 6 327 139 90 3166
Fragments arranged by size:
3166 327 139 90 55 6
NcoI C'CATG_G
Cuts at: 0 1322 3783
Size: 1322 2461
NgoMIV G'CCGG_C
Cuts at: 0 57 347 523 3783
Size: 57 290 176 3260
Fragments arranged by size:
3260 290 176 57
NheI G'CTAG_C
Cuts at: 0 1770 3783
Size: 1770 2013
NotI GC'GGCC_GC
Cuts at: 0 824 3783
Size: 824 2959
NspI r_CATG'y
Cuts at: 0 1041 1683 2559 2941 3672 3783
Size: 1041 642 876 382 731 111
Fragments arranged by size:
1041 876 731 642 382 111
PasI CC'CwG_GG
Cuts at: 0 612 3783
Size: 612 3171
PciI A'CATG_T
Cuts at: 0 2555 3668 3783
Size: 2555 1113 115
Fragments arranged by size:
2555 1113 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 942 1870 3021 3681 3783
Size: 215 727 928 1151 660 102
Fragments arranged by size:
1151 928 727 660 215 102
PfoI T'CCnGG_A
Cuts at: 0 1223 1652 2470 3783
Size: 1223 429 818 1313
Fragments arranged by size:
1313 1223 818 429
PmlI CAC'GTG
Cuts at: 0 2564 2640 3783
Size: 2564 76 1143
Fragments arranged by size:
2564 1143 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3328 3360 3783
Size: 3328 32 423
Fragments arranged by size:
3328 423 32
PpuMI rG'GwC_Cy
Cuts at: 0 2577 2918 3783
Size: 2577 341 865
Fragments arranged by size:
2577 865 341
PshAI GACnn'nnGTC
Cuts at: 0 332 3783
Size: 332 3451
PspOMI G'GGCC_C
Cuts at: 0 592 3769 3783
Size: 592 3177 14
Fragments arranged by size:
3177 592 14
PstI C_TGCA'G
Cuts at: 0 641 2462 2777 3062 3098 3783
Size: 641 1821 315 285 36 685
Fragments arranged by size:
1821 685 641 315 285 36
PvuII CAG'CTG
Cuts at: 0 801 2783 3354 3702 3783
Size: 801 1982 571 348 81
Fragments arranged by size:
1982 801 571 348 81
SacI G_AGCT'C
Cuts at: 0 1490 2822 3185 3783
Size: 1490 1332 363 598
Fragments arranged by size:
1490 1332 598 363
SacII CC_GC'GG
Cuts at: 0 153 3783
Size: 153 3630
SapI GCTCTTCn'nnn_
Cuts at: 0 1495 2449 2866 3783
Size: 1495 954 417 917
Fragments arranged by size:
1495 954 917 417
ScaI AGT'ACT
Cuts at: 0 2257 2725 3783
Size: 2257 468 1058
Fragments arranged by size:
2257 1058 468
SexAI A'CCwGG_T
Cuts at: 0 2060 3674 3783
Size: 2060 1614 109
Fragments arranged by size:
2060 1614 109
SfcI C'TryA_G
Cuts at: 0 637 2458 2661 2733 2773 3058 3094 3783
Size: 637 1821 203 72 40 285 36 689
Fragments arranged by size:
1821 689 637 285 203 72 40 36
SfoI GGC'GCC
Cuts at: 0 56 62 389 528 618 3783
Size: 56 6 327 139 90 3165
Fragments arranged by size:
3165 327 139 90 56 6
SgrAI Cr'CCGG_yG
Cuts at: 0 57 3634 3783
Size: 57 3577 149
Fragments arranged by size:
3577 149 57
SmaI CCC'GGG
Cuts at: 0 29 67 138 171 385 3783
Size: 29 38 71 33 214 3398
Fragments arranged by size:
3398 214 71 38 33 29
SmlI C'TyrA_G
Cuts at: 0 1894 3783
Size: 1894 1889
SnaBI TAC'GTA
Cuts at: 0 1534 3783
Size: 1534 2249
SphI G_CATG'C
Cuts at: 0 1041 2941 3783
Size: 1041 1900 842
Fragments arranged by size:
1900 1041 842
StuI AGG'CCT
Cuts at: 0 3100 3783
Size: 3100 683
StyI C'CwwG_G
Cuts at: 0 1322 1545 1722 1820 2069 2102 2402 2945
Size: 1322 223 177 98 249 33 300 543
Cuts at: 2945 3134 3783
Size: 189 649
Fragments arranged by size:
1322 649 543 300 249 223 189 177
98 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1165 1201 1951 2041 2186 2291 3783
Size: 1165 36 750 90 145 105 1492
Fragments arranged by size:
1492 1165 750 145 105 90 36
TatI w'GTAC_w
Cuts at: 0 1707 2255 2723 3488 3783
Size: 1707 548 468 765 295
Fragments arranged by size:
1707 765 548 468 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1176 1208 3783
Size: 1176 32 2575
Fragments arranged by size:
2575 1176 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1786 2580 3471 3783
Size: 1786 794 891 312
Fragments arranged by size:
1786 891 794 312
XmaI C'CCGG_G
Cuts at: 0 27 65 136 169 383 3783
Size: 27 38 71 33 214 3400
Fragments arranged by size:
3400 214 71 38 33 27
ZraI GAC'GTC
Cuts at: 0 1749 3783
Size: 1749 2034
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SapI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SnaBI SphI
StuI StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AscI AseI AsiSI AvrII BamHI
BciVI BclI BfrBI BglII BsiWI BsmI BspEI BsrGI
BstAPI BstBI BstZ17I ClaI DraI DraIII EcoRV FalI
FseI HindIII HpaI KpnI MfeI NdeI NruI NsiI
PacI PmeI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SbfI SfiI SgfI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI