Restriction map for
pF1KE0099
(Linear) (Six-base) MAPSORT of: KIEE0099.seq Check: 7017 from: 1 to: 801
KIEE0099.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 801
Size: 225 576
AfeI AGC'GCT
Cuts at: 0 589 765 801
Size: 589 176 36
Fragments arranged by size:
589 176 36
AhdI GACnn_n'nnGTC
Cuts at: 0 159 801
Size: 159 642
AlwNI CAG_nnn'CTG
Cuts at: 0 471 801
Size: 471 330
AvaI C'yCGr_G
Cuts at: 0 703 769 801
Size: 703 66 32
Fragments arranged by size:
703 66 32
BanI G'GyrC_C
Cuts at: 0 628 801
Size: 628 173
BanII G_rGCy'C
Cuts at: 0 91 413 449 499 801
Size: 91 322 36 50 302
Fragments arranged by size:
322 302 91 50 36
BbeI G_GCGC'C
Cuts at: 0 632 801
Size: 632 169
BglI GCCn_nnn'nGGC
Cuts at: 0 503 637 801
Size: 503 134 164
Fragments arranged by size:
503 164 134
BlpI GC'TnA_GC
Cuts at: 0 493 679 801
Size: 493 186 122
Fragments arranged by size:
493 186 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 228 511 801
Size: 159 69 283 290
Fragments arranged by size:
290 283 159 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 68 801
Size: 68 733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 757 801
Size: 361 396 44
Fragments arranged by size:
396 361 44
Bpu10I CC'TnA_GC
Cuts at: 0 760 801
Size: 760 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 625 801
Size: 598 27 176
Fragments arranged by size:
598 176 27
BsaAI yAC'GTr
Cuts at: 0 126 801
Size: 126 675
BsaHI Gr'CG_yC
Cuts at: 0 629 801
Size: 629 172
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 415 523 787 801
Size: 358 57 108 264 14
Fragments arranged by size:
358 264 108 57 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 457 801
Size: 382 75 344
Fragments arranged by size:
382 344 75
BsiEI CG_ry'CG
Cuts at: 0 522 801
Size: 522 279
BsiHKAI G_wGCw'C
Cuts at: 0 32 137 413 449 676 801
Size: 32 105 276 36 227 125
Fragments arranged by size:
276 227 125 105 36 32
Bsp1286I G_dGCh'C
Cuts at: 0 32 91 137 413 449 499 676 801
Size: 32 59 46 276 36 50 177 125
Fragments arranged by size:
276 177 125 59 50 46 36 32
BssHII G'CGCG_C
Cuts at: 0 438 458 460 524 526 528 598 801
Size: 438 20 2 64 2 2 70 203
Fragments arranged by size:
438 203 70 64 20 2 2 2
BssSI C'ACGA_G
Cuts at: 0 475 801
Size: 475 326
BstAPI GCAn_nnn'nTGC
Cuts at: 0 480 801
Size: 480 321
BstYI r'GATC_y
Cuts at: 0 336 801
Size: 336 465
Bsu36I CC'TnA_GG
Cuts at: 0 316 801
Size: 316 485
EaeI y'GGCC_r
Cuts at: 0 23 113 593 638 801
Size: 23 90 480 45 163
Fragments arranged by size:
480 163 90 45 23
EarI CTCTTCn'nnn_
Cuts at: 0 37 801
Size: 37 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 168 703 801
Size: 168 535 98
Fragments arranged by size:
535 168 98
EcoICRI GAG'CTC
Cuts at: 0 411 447 801
Size: 411 36 354
Fragments arranged by size:
411 354 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 361 757 801
Size: 225 136 396 44
Fragments arranged by size:
396 225 136 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 298 319 801
Size: 298 21 482
Fragments arranged by size:
482 298 21
EcoO109I rG'GnC_Cy
Cuts at: 0 108 312 708 801
Size: 108 204 396 93
Fragments arranged by size:
396 204 108 93
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 771 801
Size: 771 30
FspI TGC'GCA
Cuts at: 0 550 801
Size: 550 251
HaeII r_GCGC'y
Cuts at: 0 591 632 767 801
Size: 591 41 135 34
Fragments arranged by size:
591 135 41 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 321 353 801
Size: 145 32 144 32 448
Fragments arranged by size:
448 145 144 32 32
KasI G'GCGC_C
Cuts at: 0 628 801
Size: 628 173
MscI TGG'CCA
Cuts at: 0 115 801
Size: 115 686
MspA1I CmG'CkG
Cuts at: 0 146 435 801
Size: 146 289 366
Fragments arranged by size:
366 289 146
NarI GG'CG_CC
Cuts at: 0 629 801
Size: 629 172
PasI CC'CwG_GG
Cuts at: 0 92 801
Size: 92 709
PflMI CCAn_nnn'nTGG
Cuts at: 0 426 591 801
Size: 426 165 210
Fragments arranged by size:
426 210 165
PpuMI rG'GwC_Cy
Cuts at: 0 108 708 801
Size: 108 600 93
Fragments arranged by size:
600 108 93
PspXI vC'TCGA_Gb
Cuts at: 0 703 801
Size: 703 98
SacI G_AGCT'C
Cuts at: 0 413 449 801
Size: 413 36 352
Fragments arranged by size:
413 352 36
SapI GCTCTTCn'nnn_
Cuts at: 0 37 801
Size: 37 764
SfcI C'TryA_G
Cuts at: 0 567 801
Size: 567 234
SfoI GGC'GCC
Cuts at: 0 630 801
Size: 630 171
SmlI C'TyrA_G
Cuts at: 0 604 613 703 769 801
Size: 604 9 90 66 32
Fragments arranged by size:
604 90 66 32 9
StuI AGG'CCT
Cuts at: 0 722 801
Size: 722 79
StyI C'CwwG_G
Cuts at: 0 20 182 356 653 717 801
Size: 20 162 174 297 64 84
Fragments arranged by size:
297 174 162 84 64 20
TatI w'GTAC_w
Cuts at: 0 776 801
Size: 776 25
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 801
Size: 123 678
XhoI C'TCGA_G
Cuts at: 0 703 769 801
Size: 703 66 32
Fragments arranged by size:
703 66 32
Enzymes that do cut:
AcuI AfeI AhdI AlwNI AvaI BanI BanII BbeI
BglI BlpI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BssHII BssSI
BstAPI BstYI Bsu36I EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MscI
MspA1I NarI PasI PflMI PpuMI PspXI SacI SapI
SfcI SfoI SmlI StuI StyI TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmtI BsaI BsaBI
BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstEII BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I
XbaI XmaI XmnI ZraI