(Linear) (Six-base) MAPSORT of: KIEE0101.seq  Check: 4013  from: 1  to: 546



KIEE0101.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AgeI A'CCGG_T

Cuts at:      0      38     546 
   Size:         38     508
 
AleI CACnn'nnGTG

Cuts at:      0      39     487     546 
   Size:         39     448      59

  Fragments arranged by size: 

                 448      59      39
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67     546 
   Size:         67     479
 
AlwNI CAG_nnn'CTG

Cuts at:      0     400     546 
   Size:        400     146
 
ApoI r'AATT_y

Cuts at:      0      61      89     379     546 
   Size:         61      28     290     167

  Fragments arranged by size: 

                 290     167      61      28
 
AseI AT'TA_AT

Cuts at:      0     389     546 
   Size:        389     157
 
AvaI C'yCGr_G

Cuts at:      0     336     546 
   Size:        336     210
 
BanI G'GyrC_C

Cuts at:      0      78     546 
   Size:         78     468
 
BglI GCCn_nnn'nGGC

Cuts at:      0     409     546 
   Size:        409     137
 
BglII A'GATC_T

Cuts at:      0      21     546 
   Size:         21     525
 
BlpI GC'TnA_GC

Cuts at:      0     294     546 
   Size:        294     252
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     416     546 
   Size:        416     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     546 
   Size:        130     416
 
BsaBI GATnn'nnATC

Cuts at:      0     194     460     546 
   Size:        194     266      86

  Fragments arranged by size: 

                 266     194      86
 
BsaWI w'CCGG_w

Cuts at:      0      38     546 
   Size:         38     508
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     497     527     546 
   Size:        497      30      19

  Fragments arranged by size: 

                 497      30      19
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     194     546 
   Size:        194     352
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10      90     546 
   Size:         10      80     456

  Fragments arranged by size: 

                 456      80      10
 
BsrFI r'CCGG_y

Cuts at:      0      38     546 
   Size:         38     508
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     418     546 
   Size:        418     128
 
BstEII G'GTnAC_C

Cuts at:      0     480     546 
   Size:        480      66
 
BstYI r'GATC_y

Cuts at:      0      21     546 
   Size:         21     525
 
BtgI C'CryG_G

Cuts at:      0     255     274     546 
   Size:        255      19     272

  Fragments arranged by size: 

                 272     255      19
 
EaeI y'GGCC_r

Cuts at:      0     287     546 
   Size:        287     259
 
EcoRV GAT'ATC

Cuts at:      0     462     546 
   Size:        462      84
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     210     546 
   Size:        210     336
 
MscI TGG'CCA

Cuts at:      0     289     546 
   Size:        289     257
 
MslI CAynn'nnrTG

Cuts at:      0      39     487     546 
   Size:         39     448      59

  Fragments arranged by size: 

                 448      59      39
 
NcoI C'CATG_G

Cuts at:      0     255     274     546 
   Size:        255      19     272

  Fragments arranged by size: 

                 272     255      19
 
SfcI C'TryA_G

Cuts at:      0     473     546 
   Size:        473      73
 
SgrAI Cr'CCGG_yG

Cuts at:      0      38     546 
   Size:         38     508
 
SmlI C'TyrA_G

Cuts at:      0     145     546 
   Size:        145     401
 
StyI C'CwwG_G

Cuts at:      0     255     274     422     546 
   Size:        255      19     148     124

  Fragments arranged by size: 

                 255     148     124      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     497     529     546 
   Size:        497      32      17

  Fragments arranged by size: 

                 497      32      17
 

 Enzymes that do cut:

     AgeI     AleI     AlfI    AlwNI     ApoI     AseI     AvaI     BanI
     BglI    BglII     BlpI     BmrI    BpuEI    BsaBI    BsaWI    BsaXI
    BseRI     BsgI    BsrFI   BstAPI   BstEII    BstYI     BtgI     EaeI
    EcoRV     MmeI     MscI     MslI     NcoI     SfcI    SgrAI     SmlI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI