Restriction map for
pF1KE0102
(Linear) (Six-base) MAPSORT of: KIEE0102.seq Check: 2638 from: 1 to: 1077
KIEE0102.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1077
Size: 463 614
AleI CACnn'nnGTG
Cuts at: 0 391 1077
Size: 391 686
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1077
Size: 1050 27
ApaI G_GGCC'C
Cuts at: 0 661 1077
Size: 661 416
BamHI G'GATC_C
Cuts at: 0 926 1077
Size: 926 151
BanI G'GyrC_C
Cuts at: 0 55 271 316 355 520 598 943 1077
Size: 55 216 45 39 165 78 345 134
Fragments arranged by size:
345 216 165 134 78 55 45 39
BanII G_rGCy'C
Cuts at: 0 468 661 1077
Size: 468 193 416
Fragments arranged by size:
468 416 193
BclI T'GATC_A
Cuts at: 0 533 1010 1077
Size: 533 477 67
Fragments arranged by size:
533 477 67
BlpI GC'TnA_GC
Cuts at: 0 572 1077
Size: 572 505
Bme1580I G_kGCm'C
Cuts at: 0 265 276 358 523 661 683 946 1077
Size: 265 11 82 165 138 22 263 131
Fragments arranged by size:
265 263 165 138 131 82 22 11
BmgBI CAC'GTC
Cuts at: 0 99 831 1077
Size: 99 732 246
Fragments arranged by size:
732 246 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 144 265 390 565 1077
Size: 19 125 121 125 175 512
Fragments arranged by size:
512 175 125 125 121 19
Bpu10I CC'TnA_GC
Cuts at: 0 623 1077
Size: 623 454
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 1077
Size: 238 30 809
Fragments arranged by size:
809 238 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 186 1077
Size: 186 891
BseYI C'CCAG_C
Cuts at: 0 594 648 653 1077
Size: 594 54 5 424
Fragments arranged by size:
594 424 54 5
Bsp1286I G_dGCh'C
Cuts at: 0 265 276 358 468 523 661 683 946
Size: 265 11 82 110 55 138 22 263
Cuts at: 946 1077
Size: 131
Fragments arranged by size:
265 263 138 131 110 82 55 22
11
BspHI T'CATG_A
Cuts at: 0 83 1007 1077
Size: 83 924 70
Fragments arranged by size:
924 83 70
BsrBI CCG'CTC
Cuts at: 0 374 1077
Size: 374 703
BsrDI GCAATG_nn'
Cuts at: 0 240 1077
Size: 240 837
BsrFI r'CCGG_y
Cuts at: 0 1061 1077
Size: 1061 16
BssHII G'CGCG_C
Cuts at: 0 503 1077
Size: 503 574
BssSI C'ACGA_G
Cuts at: 0 349 1077
Size: 349 728
BstBI TT'CG_AA
Cuts at: 0 609 1077
Size: 609 468
BstEII G'GTnAC_C
Cuts at: 0 69 1077
Size: 69 1008
BstYI r'GATC_y
Cuts at: 0 926 1077
Size: 926 151
Bsu36I CC'TnA_GG
Cuts at: 0 51 1077
Size: 51 1026
BtgI C'CryG_G
Cuts at: 0 134 320 491 1077
Size: 134 186 171 586
Fragments arranged by size:
586 186 171 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 880 1077
Size: 880 197
BtsI GCAGTG_nn'
Cuts at: 0 425 623 662 815 1077
Size: 425 198 39 153 262
Fragments arranged by size:
425 262 198 153 39
EaeI y'GGCC_r
Cuts at: 0 137 1077
Size: 137 940
EarI CTCTTCn'nnn_
Cuts at: 0 85 715 1077
Size: 85 630 362
Fragments arranged by size:
630 362 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 144 265 390 463 565 1077
Size: 19 125 121 125 73 102 512
Fragments arranged by size:
512 125 125 121 102 73 19
EcoO109I rG'GnC_Cy
Cuts at: 0 12 362 657 658 1077
Size: 12 350 295 1 419
Fragments arranged by size:
419 350 295 12 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 1077
Size: 972 105
HaeII r_GCGC'y
Cuts at: 0 639 1077
Size: 639 438
HindIII A'AGCT_T
Cuts at: 0 1035 1077
Size: 1035 42
MscI TGG'CCA
Cuts at: 0 139 1077
Size: 139 938
MslI CAynn'nnrTG
Cuts at: 0 391 1077
Size: 391 686
MspA1I CmG'CkG
Cuts at: 0 126 278 1077
Size: 126 152 799
Fragments arranged by size:
799 152 126
NcoI C'CATG_G
Cuts at: 0 134 320 491 1077
Size: 134 186 171 586
Fragments arranged by size:
586 186 171 134
NspI r_CATG'y
Cuts at: 0 580 898 1077
Size: 580 318 179
Fragments arranged by size:
580 318 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 167 1077
Size: 42 125 910
Fragments arranged by size:
910 125 42
PfoI T'CCnGG_A
Cuts at: 0 242 1077
Size: 242 835
PpuMI rG'GwC_Cy
Cuts at: 0 12 362 1077
Size: 12 350 715
Fragments arranged by size:
715 350 12
PspOMI G'GGCC_C
Cuts at: 0 657 1077
Size: 657 420
PstI C_TGCA'G
Cuts at: 0 119 569 618 810 1077
Size: 119 450 49 192 267
Fragments arranged by size:
450 267 192 119 49
SapI GCTCTTCn'nnn_
Cuts at: 0 715 1077
Size: 715 362
SexAI A'CCwGG_T
Cuts at: 0 286 1077
Size: 286 791
SfcI C'TryA_G
Cuts at: 0 115 565 614 806 1077
Size: 115 450 49 192 271
Fragments arranged by size:
450 271 192 115 49
StuI AGG'CCT
Cuts at: 0 448 1077
Size: 448 629
StyI C'CwwG_G
Cuts at: 0 134 320 491 947 1077
Size: 134 186 171 456 130
Fragments arranged by size:
456 186 171 134 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 504 586 1077
Size: 504 82 491
Fragments arranged by size:
504 491 82
TatI w'GTAC_w
Cuts at: 0 695 1077
Size: 695 382
XmnI GAAnn'nnTTC
Cuts at: 0 448 1077
Size: 448 629
Enzymes that do cut:
AcuI AleI AlwNI ApaI BamHI BanI BanII BclI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaXI BseRI BseYI
Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI
Eco57MI EcoO109I FalI HaeII HindIII MscI MslI MspA1I
NcoI NspI PflMI PfoI PpuMI PspOMI PstI SapI
SexAI SfcI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrGI BstAPI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI