(Linear) (Six-base) MAPSORT of: KIEE0104.seq  Check: 2946  from: 1  to: 1386



KIEE0104.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:      0    1216    1386 
   Size:       1216     170
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      83     731    1386 
   Size:         43      40     648     655

  Fragments arranged by size: 

                 655     648      43      40
 
AlwNI CAG_nnn'CTG

Cuts at:      0      61    1281    1386 
   Size:         61    1220     105

  Fragments arranged by size: 

                1220     105      61
 
ApoI r'AATT_y

Cuts at:      0     715    1386 
   Size:        715     671
 
AseI AT'TA_AT

Cuts at:      0    1351    1386 
   Size:       1351      35
 
AvaI C'yCGr_G

Cuts at:      0     648    1386 
   Size:        648     738
 
BanI G'GyrC_C

Cuts at:      0     170    1386 
   Size:        170    1216
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     920    1386 
   Size:        920     466
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1016    1386 
   Size:       1016     370
 
BglII A'GATC_T

Cuts at:      0     814    1386 
   Size:        814     572
 
BlpI GC'TnA_GC

Cuts at:      0    1200    1386 
   Size:       1200     186
 
Bme1580I G_kGCm'C

Cuts at:      0     173    1386 
   Size:        173    1213
 
BmtI G_CTAG'C

Cuts at:      0     421    1386 
   Size:        421     965
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1097    1386 
   Size:       1097     289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     791    1386 
   Size:        217     574     595

  Fragments arranged by size: 

                 595     574     217
 
BsaBI GATnn'nnATC

Cuts at:      0     467    1386 
   Size:        467     919
 
BsaHI Gr'CG_yC

Cuts at:      0     935    1386 
   Size:        935     451
 
BsaWI w'CCGG_w

Cuts at:      0     380    1386 
   Size:        380    1006
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1118    1148    1386 
   Size:       1118      30     238

  Fragments arranged by size: 

                1118     238      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     670    1386 
   Size:        670     716
 
BseYI C'CCAG_C

Cuts at:      0     566     793    1386 
   Size:        566     227     593

  Fragments arranged by size: 

                 593     566     227
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227    1386 
   Size:        227    1159
 
BsmI GAATG_Cn'

Cuts at:      0    1312    1386 
   Size:       1312      74
 
Bsp1286I G_dGCh'C

Cuts at:      0     173    1386 
   Size:        173    1213
 
BstYI r'GATC_y

Cuts at:      0     696     814    1386 
   Size:        696     118     572

  Fragments arranged by size: 

                 696     572     118
 
BtgI C'CryG_G

Cuts at:      0    1165    1386 
   Size:       1165     221
 
BtsI GCAGTG_nn'

Cuts at:      0    1217    1386 
   Size:       1217     169
 
DraI TTT'AAA

Cuts at:      0     467     908    1386 
   Size:        467     441     478

  Fragments arranged by size: 

                 478     467     441
 
EaeI y'GGCC_r

Cuts at:      0    1137    1168    1386 
   Size:       1137      31     218

  Fragments arranged by size: 

                1137     218      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     116    1109    1386 
   Size:        116     993     277

  Fragments arranged by size: 

                 993     277     116
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43      83     731    1097    1386 
   Size:         43      40     648     366     289

  Fragments arranged by size: 

                 648     366     289      43      40
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1278    1386 
   Size:       1278     108
 
EcoRI G'AATT_C

Cuts at:      0     715    1386 
   Size:        715     671
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     541     600    1386 
   Size:        541      59     786

  Fragments arranged by size: 

                 786     541      59
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     206     238    1262    1294    1386 
   Size:        206      32    1024      32      92

  Fragments arranged by size: 

                1024     206      92      32      32
 
HincII GTy'rAC

Cuts at:      0      83    1162    1233    1386 
   Size:         83    1079      71     153

  Fragments arranged by size: 

                1079     153      83      71
 
HpaI GTT'AAC

Cuts at:      0      83    1386 
   Size:         83    1303
 
MfeI C'AATT_G

Cuts at:      0     218     775    1386 
   Size:        218     557     611

  Fragments arranged by size: 

                 611     557     218
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     531    1386 
   Size:        531     855
 
MscI TGG'CCA

Cuts at:      0    1139    1170    1386 
   Size:       1139      31     216

  Fragments arranged by size: 

                1139     216      31
 
MslI CAynn'nnrTG

Cuts at:      0     995    1386 
   Size:        995     391
 
MspA1I CmG'CkG

Cuts at:      0      58     534    1259    1386 
   Size:         58     476     725     127

  Fragments arranged by size: 

                 725     476     127      58
 
NcoI C'CATG_G

Cuts at:      0    1165    1386 
   Size:       1165     221
 
NheI G'CTAG_C

Cuts at:      0     417    1386 
   Size:        417     969
 
NspI r_CATG'y

Cuts at:      0    1047    1386 
   Size:       1047     339
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     859    1386 
   Size:        859     527
 
PfoI T'CCnGG_A

Cuts at:      0    1280    1386 
   Size:       1280     106
 
PpuMI rG'GwC_Cy

Cuts at:      0    1278    1386 
   Size:       1278     108
 
PsiI TTA'TAA

Cuts at:      0     272    1386 
   Size:        272    1114
 
PvuII CAG'CTG

Cuts at:      0      58     534    1386 
   Size:         58     476     852

  Fragments arranged by size: 

                 852     476      58
 
SfcI C'TryA_G

Cuts at:      0    1217    1386 
   Size:       1217     169
 
SmlI C'TyrA_G

Cuts at:      0     196     806    1386 
   Size:        196     610     580

  Fragments arranged by size: 

                 610     580     196
 
StyI C'CwwG_G

Cuts at:      0     319     473    1165    1386 
   Size:        319     154     692     221

  Fragments arranged by size: 

                 692     319     221     154
 
SwaI ATTT'AAAT

Cuts at:      0     467    1386 
   Size:        467     919
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     188    1386 
   Size:        188    1198
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     AseI     AvaI     BanI     BbsI
     BglI    BglII     BlpI Bme1580I     BmtI     BpmI    BpuEI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI     BsmI Bsp1286I
    BstYI     BtgI     BtsI     DraI     EaeI     EarI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I   HincII     HpaI     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NheI     NspI    PflMI     PfoI    PpuMI
     PsiI    PvuII     SfcI     SmlI     StyI     SwaI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI