(Linear) (Six-base) MAPSORT of: KIEE0105.seq  Check: 6885  from: 1  to: 543



KIEE0105.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

ApoI r'AATT_y

Cuts at:      0     431     543 
   Size:        431     112
 
BanI G'GyrC_C

Cuts at:      0     496     543 
   Size:        496      47
 
BanII G_rGCy'C

Cuts at:      0     121     543 
   Size:        121     422
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539     543 
   Size:        539       4
 
BlpI GC'TnA_GC

Cuts at:      0     115     217     543 
   Size:        115     102     326

  Fragments arranged by size: 

                 326     115     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     459     543 
   Size:        459      84
 
BsaBI GATnn'nnATC

Cuts at:      0     304     543 
   Size:        304     239
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     543 
   Size:         40     503
 
BsmI GAATG_Cn'

Cuts at:      0     283     543 
   Size:        283     260
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     543 
   Size:        121     422
 
BsrDI GCAATG_nn'

Cuts at:      0     278     543 
   Size:        278     265
 
BstYI r'GATC_y

Cuts at:      0     316     543 
   Size:        316     227
 
BtsI GCAGTG_nn'

Cuts at:      0     201     543 
   Size:        201     342
 
HaeII r_GCGC'y

Cuts at:      0      91     266     543 
   Size:         91     175     277

  Fragments arranged by size: 

                 277     175      91
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     225     257     543 
   Size:        225      32     286

  Fragments arranged by size: 

                 286     225      32
 
MspA1I CmG'CkG

Cuts at:      0      17     136     543 
   Size:         17     119     407

  Fragments arranged by size: 

                 407     119      17
 
PvuII CAG'CTG

Cuts at:      0     136     543 
   Size:        136     407
 
ScaI AGT'ACT

Cuts at:      0     148     543 
   Size:        148     395
 
SfcI C'TryA_G

Cuts at:      0     333     543 
   Size:        333     210
 
TatI w'GTAC_w

Cuts at:      0     146     543 
   Size:        146     397
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     193     225     543 
   Size:        193      32     318

  Fragments arranged by size: 

                 318     193      32
 

 Enzymes that do cut:

     ApoI     BanI    BanII     BbsI     BlpI     BmrI    BsaBI    BseRI
     BsmI Bsp1286I    BsrDI    BstYI     BtsI    HaeII    Hin4I   MspA1I
    PvuII     ScaI     SfcI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI