Restriction map for
pF1KE0106
(Linear) (Six-base) MAPSORT of: KIEE0106.seq Check: 5258 from: 1 to: 843
KIEE0106.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 843
Size: 379 464
AlwNI CAG_nnn'CTG
Cuts at: 0 125 273 423 738 843
Size: 125 148 150 315 105
Fragments arranged by size:
315 150 148 125 105
ApaI G_GGCC'C
Cuts at: 0 629 843
Size: 629 214
ApoI r'AATT_y
Cuts at: 0 715 843
Size: 715 128
BanI G'GyrC_C
Cuts at: 0 325 532 843
Size: 325 207 311
Fragments arranged by size:
325 311 207
BanII G_rGCy'C
Cuts at: 0 231 629 843
Size: 231 398 214
Fragments arranged by size:
398 231 214
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 843
Size: 457 386
BbvCI CC'TCA_GC
Cuts at: 0 603 843
Size: 603 240
Bme1580I G_kGCm'C
Cuts at: 0 629 843
Size: 629 214
BmrI ACTGGGnnnn_n'
Cuts at: 0 261 843
Size: 261 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 787 843
Size: 122 665 56
Fragments arranged by size:
665 122 56
Bpu10I CC'TnA_GC
Cuts at: 0 235 603 843
Size: 235 368 240
Fragments arranged by size:
368 240 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 733 843
Size: 298 435 110
Fragments arranged by size:
435 298 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 27 215 843
Size: 27 188 628
Fragments arranged by size:
628 188 27
BsaHI Gr'CG_yC
Cuts at: 0 106 366 843
Size: 106 260 477
Fragments arranged by size:
477 260 106
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 843
Size: 125 718
BseYI C'CCAG_C
Cuts at: 0 607 660 843
Size: 607 53 183
Fragments arranged by size:
607 183 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 843
Size: 259 584
BsiEI CG_ry'CG
Cuts at: 0 290 843
Size: 290 553
BsiHKAI G_wGCw'C
Cuts at: 0 157 685 773 843
Size: 157 528 88 70
Fragments arranged by size:
528 157 88 70
Bsp1286I G_dGCh'C
Cuts at: 0 157 231 629 685 773 843
Size: 157 74 398 56 88 70
Fragments arranged by size:
398 157 88 74 70 56
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 843
Size: 302 541
BsrFI r'CCGG_y
Cuts at: 0 248 843
Size: 248 595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 777 843
Size: 738 39 66
Fragments arranged by size:
738 66 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 835 843
Size: 835 8
BstYI r'GATC_y
Cuts at: 0 548 843
Size: 548 295
BtgI C'CryG_G
Cuts at: 0 28 843
Size: 28 815
BtsI GCAGTG_nn'
Cuts at: 0 357 843
Size: 357 486
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 620 655 843
Size: 620 35 188
Fragments arranged by size:
620 188 35
DraIII CAC_nnn'GTG
Cuts at: 0 777 843
Size: 777 66
EaeI y'GGCC_r
Cuts at: 0 287 432 518 837 843
Size: 287 145 86 319 6
Fragments arranged by size:
319 287 145 86 6
EagI C'GGCC_G
Cuts at: 0 287 843
Size: 287 556
EarI CTCTTCn'nnn_
Cuts at: 0 482 843
Size: 482 361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 379 787 843
Size: 122 257 408 56
Fragments arranged by size:
408 257 122 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 140 277 373 843
Size: 140 137 96 470
Fragments arranged by size:
470 140 137 96
EcoO109I rG'GnC_Cy
Cuts at: 0 420 591 612 626 843
Size: 420 171 21 14 217
Fragments arranged by size:
420 217 171 21 14
EcoRI G'AATT_C
Cuts at: 0 715 843
Size: 715 128
HaeII r_GCGC'y
Cuts at: 0 249 843
Size: 249 594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 459 491 843
Size: 459 32 352
Fragments arranged by size:
459 352 32
HincII GTy'rAC
Cuts at: 0 486 525 843
Size: 486 39 318
Fragments arranged by size:
486 318 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 629 843
Size: 25 604 214
Fragments arranged by size:
604 214 25
MscI TGG'CCA
Cuts at: 0 434 839 843
Size: 434 405 4
Fragments arranged by size:
434 405 4
MspA1I CmG'CkG
Cuts at: 0 303 507 607 660 843
Size: 303 204 100 53 183
Fragments arranged by size:
303 204 183 100 53
NaeI GCC'GGC
Cuts at: 0 250 843
Size: 250 593
NgoMIV G'CCGG_C
Cuts at: 0 248 843
Size: 248 595
PasI CC'CwG_GG
Cuts at: 0 622 843
Size: 622 221
PpuMI rG'GwC_Cy
Cuts at: 0 420 591 612 843
Size: 420 171 21 231
Fragments arranged by size:
420 231 171 21
PspOMI G'GGCC_C
Cuts at: 0 625 843
Size: 625 218
PstI C_TGCA'G
Cuts at: 0 61 734 843
Size: 61 673 109
Fragments arranged by size:
673 109 61
PvuII CAG'CTG
Cuts at: 0 507 607 660 843
Size: 507 100 53 183
Fragments arranged by size:
507 183 100 53
SapI GCTCTTCn'nnn_
Cuts at: 0 482 843
Size: 482 361
SfcI C'TryA_G
Cuts at: 0 57 655 730 843
Size: 57 598 75 113
Fragments arranged by size:
598 113 75 57
SmlI C'TyrA_G
Cuts at: 0 313 748 843
Size: 313 435 95
Fragments arranged by size:
435 313 95
StyI C'CwwG_G
Cuts at: 0 332 843
Size: 332 511
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 280 550 843
Size: 239 41 270 293
Fragments arranged by size:
293 270 239 41
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BanI BanII BbsI BbvCI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI
BstXI BstYI BtgI BtsI CspCI DraIII EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
MmeI MscI MspA1I NaeI NgoMIV PasI PpuMI PspOMI
PstI PvuII SapI SfcI SmlI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EciI EcoICRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI