(Linear) (Six-base) MAPSORT of: KIEE0109.seq  Check: 1950  from: 1  to: 408



KIEE0109.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     250     408 
   Size:        250     158
 
ApoI r'AATT_y

Cuts at:      0     102     408 
   Size:        102     306
 
AvaI C'yCGr_G

Cuts at:      0     179     408 
   Size:        179     229
 
BclI T'GATC_A

Cuts at:      0     278     408 
   Size:        278     130
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      91     408 
   Size:         91     317
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     124     408 
   Size:         14     110     284

  Fragments arranged by size: 

                 284     110      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     408 
   Size:        292     116
 
BseYI C'CCAG_C

Cuts at:      0     214     408 
   Size:        214     194
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     308     408 
   Size:        308     100
 
BtgI C'CryG_G

Cuts at:      0     359     408 
   Size:        359      49
 
EaeI y'GGCC_r

Cuts at:      0     146     408 
   Size:        146     262
 
EarI CTCTTCn'nnn_

Cuts at:      0     325     403     408 
   Size:        325      78       5

  Fragments arranged by size: 

                 325      78       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      14     124     408 
   Size:         14     110     284

  Fragments arranged by size: 

                 284     110      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     352     408 
   Size:        352      56
 
EcoRI G'AATT_C

Cuts at:      0     102     408 
   Size:        102     306
 
MscI TGG'CCA

Cuts at:      0     148     408 
   Size:        148     260
 
MspA1I CmG'CkG

Cuts at:      0      44     408 
   Size:         44     364
 
NcoI C'CATG_G

Cuts at:      0     359     408 
   Size:        359      49
 
SapI GCTCTTCn'nnn_

Cuts at:      0     325     408 
   Size:        325      83
 
SfcI C'TryA_G

Cuts at:      0     123     408 
   Size:        123     285
 
SmaI CCC'GGG

Cuts at:      0     181     408 
   Size:        181     227
 
SmlI C'TyrA_G

Cuts at:      0     307     408 
   Size:        307     101
 
StuI AGG'CCT

Cuts at:      0      69     408 
   Size:         69     339
 
StyI C'CwwG_G

Cuts at:      0     359     408 
   Size:        359      49
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231     408 
   Size:        231     177
 
XmaI C'CCGG_G

Cuts at:      0     179     408 
   Size:        179     229
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI     BclI     BmrI     BpmI    BpuEI    BseYI
    BsmBI     BtgI     EaeI     EarI  Eco57MI    EcoNI    EcoRI     MscI
   MspA1I     NcoI     SapI     SfcI     SmaI     SmlI     StuI     StyI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmnI     ZraI