Restriction map for
pF1KE0111
(Linear) (Six-base) MAPSORT of: KIEE0111.seq Check: 6839 from: 1 to: 1038
KIEE0111.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 327 1038
Size: 133 194 711
Fragments arranged by size:
711 194 133
AfeI AGC'GCT
Cuts at: 0 340 1038
Size: 340 698
AhdI GACnn_n'nnGTC
Cuts at: 0 53 1038
Size: 53 985
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 728 1038
Size: 728 310
AlwNI CAG_nnn'CTG
Cuts at: 0 481 666 852 894 1038
Size: 481 185 186 42 144
Fragments arranged by size:
481 186 185 144 42
ApaI G_GGCC'C
Cuts at: 0 13 32 144 1038
Size: 13 19 112 894
Fragments arranged by size:
894 112 19 13
AvaI C'yCGr_G
Cuts at: 0 24 97 241 431 1038
Size: 24 73 144 190 607
Fragments arranged by size:
607 190 144 73 24
AvrII C'CTAG_G
Cuts at: 0 255 1038
Size: 255 783
BanI G'GyrC_C
Cuts at: 0 92 184 1038
Size: 92 92 854
Fragments arranged by size:
854 92 92
BanII G_rGCy'C
Cuts at: 0 13 32 144 636 942 1038
Size: 13 19 112 492 306 96
Fragments arranged by size:
492 306 112 96 19 13
BbeI G_GCGC'C
Cuts at: 0 96 1038
Size: 96 942
BbsI GAAGACnn'nnnn_
Cuts at: 0 1011 1038
Size: 1011 27
BglI GCCn_nnn'nGGC
Cuts at: 0 215 1038
Size: 215 823
BglII A'GATC_T
Cuts at: 0 998 1038
Size: 998 40
BlpI GC'TnA_GC
Cuts at: 0 956 1038
Size: 956 82
Bme1580I G_kGCm'C
Cuts at: 0 13 32 144 1038
Size: 13 19 112 894
Fragments arranged by size:
894 112 19 13
BmgBI CAC'GTC
Cuts at: 0 228 1038
Size: 228 810
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 267 1038
Size: 267 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 433 859 1038
Size: 220 213 426 179
Fragments arranged by size:
426 220 213 179
Bpu10I CC'TnA_GC
Cuts at: 0 575 1038
Size: 575 463
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 259 1038
Size: 71 188 779
Fragments arranged by size:
779 188 71
BsaHI Gr'CG_yC
Cuts at: 0 93 218 1038
Size: 93 125 820
Fragments arranged by size:
820 125 93
BsaWI w'CCGG_w
Cuts at: 0 965 1038
Size: 965 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 141 171 1038
Size: 141 30 867
Fragments arranged by size:
867 141 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1000 1038
Size: 1000 38
BseYI C'CCAG_C
Cuts at: 0 443 548 919 1038
Size: 443 105 371 119
Fragments arranged by size:
443 371 119 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1038
Size: 997 41
BsiEI CG_ry'CG
Cuts at: 0 76 138 1038
Size: 76 62 900
Fragments arranged by size:
900 76 62
BsiHKAI G_wGCw'C
Cuts at: 0 707 1038
Size: 707 331
BsmI GAATG_Cn'
Cuts at: 0 645 1038
Size: 645 393
Bsp1286I G_dGCh'C
Cuts at: 0 13 32 144 636 707 942 1038
Size: 13 19 112 492 71 235 96
Fragments arranged by size:
492 235 112 96 71 19 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 651 969 1038
Size: 651 318 69
Fragments arranged by size:
651 318 69
BsrBI CCG'CTC
Cuts at: 0 157 1038
Size: 157 881
BssSI C'ACGA_G
Cuts at: 0 617 1038
Size: 617 421
BstAPI GCAn_nnn'nTGC
Cuts at: 0 622 1038
Size: 622 416
BstXI CCAn_nnnn'nTGG
Cuts at: 0 457 1038
Size: 457 581
BstYI r'GATC_y
Cuts at: 0 194 248 998 1038
Size: 194 54 750 40
Fragments arranged by size:
750 194 54 40
Bsu36I CC'TnA_GG
Cuts at: 0 290 313 1038
Size: 290 23 725
Fragments arranged by size:
725 290 23
BtgI C'CryG_G
Cuts at: 0 162 1038
Size: 162 876
BtsI GCAGTG_nn'
Cuts at: 0 655 1038
Size: 655 383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 725 760 1038
Size: 725 35 278
Fragments arranged by size:
725 278 35
EaeI y'GGCC_r
Cuts at: 0 73 207 1038
Size: 73 134 831
Fragments arranged by size:
831 134 73
EagI C'GGCC_G
Cuts at: 0 73 1038
Size: 73 965
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 333 1038
Size: 333 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 220 327 433 859 1038
Size: 133 87 107 106 426 179
Fragments arranged by size:
426 179 133 107 106 87
EcoO109I rG'GnC_Cy
Cuts at: 0 9 28 141 259 266 285 383 565
Size: 9 19 113 118 7 19 98 182
Cuts at: 565 663 759 1038
Size: 98 96 279
Fragments arranged by size:
279 182 118 113 98 98 96 19
19 9 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 138 1038
Size: 138 900
HaeII r_GCGC'y
Cuts at: 0 96 342 405 1038
Size: 96 246 63 633
Fragments arranged by size:
633 246 96 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 39 69 71 101 990 1022 1038
Size: 30 9 30 2 30 889 32 16
Fragments arranged by size:
889 32 30 30 30 16 9 2
KasI G'GCGC_C
Cuts at: 0 92 1038
Size: 92 946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 728 1038
Size: 158 570 310
Fragments arranged by size:
570 310 158
MslI CAynn'nnrTG
Cuts at: 0 710 832 1038
Size: 710 122 206
Fragments arranged by size:
710 206 122
MspA1I CmG'CkG
Cuts at: 0 509 690 849 891 1038
Size: 509 181 159 42 147
Fragments arranged by size:
509 181 159 147 42
NarI GG'CG_CC
Cuts at: 0 93 1038
Size: 93 945
NspI r_CATG'y
Cuts at: 0 837 1038
Size: 837 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 852 1038
Size: 852 186
PfoI T'CCnGG_A
Cuts at: 0 197 449 1038
Size: 197 252 589
Fragments arranged by size:
589 252 197
PpuMI rG'GwC_Cy
Cuts at: 0 259 285 565 663 759 1038
Size: 259 26 280 98 96 279
Fragments arranged by size:
280 279 259 98 96 26
PspOMI G'GGCC_C
Cuts at: 0 9 28 140 1038
Size: 9 19 112 898
Fragments arranged by size:
898 112 19 9
PspXI vC'TCGA_Gb
Cuts at: 0 97 241 1038
Size: 97 144 797
Fragments arranged by size:
797 144 97
PstI C_TGCA'G
Cuts at: 0 662 1038
Size: 662 376
PvuII CAG'CTG
Cuts at: 0 891 1038
Size: 891 147
SexAI A'CCwGG_T
Cuts at: 0 561 1038
Size: 561 477
SfcI C'TryA_G
Cuts at: 0 66 658 1038
Size: 66 592 380
Fragments arranged by size:
592 380 66
SfoI GGC'GCC
Cuts at: 0 94 1038
Size: 94 944
SmaI CCC'GGG
Cuts at: 0 433 1038
Size: 433 605
SmlI C'TyrA_G
Cuts at: 0 86 97 241 274 1038
Size: 86 11 144 33 764
Fragments arranged by size:
764 144 86 33 11
SphI G_CATG'C
Cuts at: 0 837 1038
Size: 837 201
StuI AGG'CCT
Cuts at: 0 587 1038
Size: 587 451
StyI C'CwwG_G
Cuts at: 0 255 1038
Size: 255 783
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 423 1038
Size: 166 257 615
Fragments arranged by size:
615 257 166
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 141 173 647 679 1038
Size: 141 32 474 32 359
Fragments arranged by size:
474 359 141 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 477 1038
Size: 477 561
XhoI C'TCGA_G
Cuts at: 0 97 241 1038
Size: 97 144 797
Fragments arranged by size:
797 144 97
XmaI C'CCGG_G
Cuts at: 0 431 1038
Size: 431 607
XmnI GAAnn'nnTTC
Cuts at: 0 340 1038
Size: 340 698
Enzymes that do cut:
AcuI AfeI AhdI AlfI AlwNI ApaI AvaI AvrII
BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI EaeI EagI EciI Eco57MI EcoO109I FalI HaeII
Hin4I KasI MmeI MslI MspA1I NarI NspI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SexAI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TstI
Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BmrI BmtI
BsaI BsaAI BsaBI BsiWI BsmBI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI ClaI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TatI XbaI XcmI ZraI