Restriction map for
pF1KE0114
(Linear) (Six-base) MAPSORT of: KIEE0114.seq Check: 1258 from: 1 to: 924
KIEE0114.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 0 161 924
Size: 161 763
AccI GT'mk_AC
Cuts at: 0 47 924
Size: 47 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 526 850 924
Size: 490 36 324 74
Fragments arranged by size:
490 324 74 36
AlwNI CAG_nnn'CTG
Cuts at: 0 465 522 786 924
Size: 465 57 264 138
Fragments arranged by size:
465 264 138 57
ApaLI G'TGCA_C
Cuts at: 0 441 924
Size: 441 483
BanI G'GyrC_C
Cuts at: 0 3 924
Size: 3 921
BanII G_rGCy'C
Cuts at: 0 242 924
Size: 242 682
BbeI G_GCGC'C
Cuts at: 0 7 924
Size: 7 917
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 924
Size: 153 771
BbvCI CC'TCA_GC
Cuts at: 0 704 924
Size: 704 220
Bme1580I G_kGCm'C
Cuts at: 0 445 924
Size: 445 479
BmgBI CAC'GTC
Cuts at: 0 147 339 924
Size: 147 192 585
Fragments arranged by size:
585 192 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 457 924
Size: 226 231 467
Fragments arranged by size:
467 231 226
Bpu10I CC'TnA_GC
Cuts at: 0 236 704 924
Size: 236 468 220
Fragments arranged by size:
468 236 220
BsaHI Gr'CG_yC
Cuts at: 0 4 158 924
Size: 4 154 766
Fragments arranged by size:
766 154 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 216 924
Size: 216 708
BseYI C'CCAG_C
Cuts at: 0 545 692 779 824 916 924
Size: 545 147 87 45 92 8
Fragments arranged by size:
545 147 92 87 45 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 478 924
Size: 403 75 446
Fragments arranged by size:
446 403 75
BsiHKAI G_wGCw'C
Cuts at: 0 242 445 637 924
Size: 242 203 192 287
Fragments arranged by size:
287 242 203 192
BsmI GAATG_Cn'
Cuts at: 0 518 924
Size: 518 406
BsmBI CGTCTCn'nnnn_
Cuts at: 0 137 745 924
Size: 137 608 179
Fragments arranged by size:
608 179 137
Bsp1286I G_dGCh'C
Cuts at: 0 242 445 637 924
Size: 242 203 192 287
Fragments arranged by size:
287 242 203 192
BssSI C'ACGA_G
Cuts at: 0 277 924
Size: 277 647
BstAPI GCAn_nnn'nTGC
Cuts at: 0 465 924
Size: 465 459
BstYI r'GATC_y
Cuts at: 0 461 924
Size: 461 463
Bsu36I CC'TnA_GG
Cuts at: 0 800 924
Size: 800 124
BtgI C'CryG_G
Cuts at: 0 575 924
Size: 575 349
BtsI GCAGTG_nn'
Cuts at: 0 103 554 924
Size: 103 451 370
Fragments arranged by size:
451 370 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 720 755 924
Size: 720 35 169
Fragments arranged by size:
720 169 35
EaeI y'GGCC_r
Cuts at: 0 302 365 572 924
Size: 302 63 207 352
Fragments arranged by size:
352 302 207 63
EarI CTCTTCn'nnn_
Cuts at: 0 502 924
Size: 502 422
EcoICRI GAG'CTC
Cuts at: 0 240 924
Size: 240 684
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 457 490 526 850 924
Size: 226 231 33 36 324 74
Fragments arranged by size:
324 231 226 74 36 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 715 924
Size: 526 189 209
Fragments arranged by size:
526 209 189
EcoO109I rG'GnC_Cy
Cuts at: 0 519 708 845 924
Size: 519 189 137 79
Fragments arranged by size:
519 189 137 79
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 90 924
Size: 90 834
FspI TGC'GCA
Cuts at: 0 253 924
Size: 253 671
FspAI rTGC'GCAy
Cuts at: 0 253 924
Size: 253 671
HaeII r_GCGC'y
Cuts at: 0 7 727 923 924
Size: 7 720 196 1
Fragments arranged by size:
720 196 7 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 924
Size: 619 32 273
Fragments arranged by size:
619 273 32
HincII GTy'rAC
Cuts at: 0 762 924
Size: 762 162
KasI G'GCGC_C
Cuts at: 0 3 924
Size: 3 921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 924
Size: 265 659
MscI TGG'CCA
Cuts at: 0 574 924
Size: 574 350
MslI CAynn'nnrTG
Cuts at: 0 448 454 924
Size: 448 6 470
Fragments arranged by size:
470 448 6
MspA1I CmG'CkG
Cuts at: 0 117 692 703 828 924
Size: 117 575 11 125 96
Fragments arranged by size:
575 125 117 96 11
NarI GG'CG_CC
Cuts at: 0 4 924
Size: 4 920
NspI r_CATG'y
Cuts at: 0 258 924
Size: 258 666
PasI CC'CwG_GG
Cuts at: 0 684 924
Size: 684 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 560 621 786 924
Size: 560 61 165 138
Fragments arranged by size:
560 165 138 61
PfoI T'CCnGG_A
Cuts at: 0 206 614 924
Size: 206 408 310
Fragments arranged by size:
408 310 206
PpuMI rG'GwC_Cy
Cuts at: 0 519 845 924
Size: 519 326 79
Fragments arranged by size:
519 326 79
PshAI GACnn'nnGTC
Cuts at: 0 757 924
Size: 757 167
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 61 93 924
Size: 61 32 831
Fragments arranged by size:
831 61 32
PstI C_TGCA'G
Cuts at: 0 596 719 924
Size: 596 123 205
Fragments arranged by size:
596 205 123
PvuII CAG'CTG
Cuts at: 0 828 924
Size: 828 96
SacI G_AGCT'C
Cuts at: 0 242 924
Size: 242 682
SfcI C'TryA_G
Cuts at: 0 592 715 904 924
Size: 592 123 189 20
Fragments arranged by size:
592 189 123 20
SfoI GGC'GCC
Cuts at: 0 5 924
Size: 5 919
StyI C'CwwG_G
Cuts at: 0 875 924
Size: 875 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 329 559 924
Size: 329 230 365
Fragments arranged by size:
365 329 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 183 215 924
Size: 183 32 709
Fragments arranged by size:
709 183 32
XmnI GAAnn'nnTTC
Cuts at: 0 73 924
Size: 73 851
ZraI GAC'GTC
Cuts at: 0 159 924
Size: 159 765
Enzymes that do cut:
AatII AccI AcuI AlwNI ApaLI BanI BanII BbeI
BbsI BbvCI Bme1580I BmgBI BpmI Bpu10I BsaHI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BssSI BstAPI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NspI
PasI PflMI PfoI PpuMI PshAI PsrI PstI PvuII
SacI SfcI SfoI StyI TaqII TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI