(Linear) (Six-base) MAPSORT of: KIEE0115.seq  Check: 3038  from: 1  to: 684



KIEE0115.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AflII C'TTAA_G

Cuts at:      0     441     582     684 
   Size:        441     141     102

  Fragments arranged by size: 

                 441     141     102
 
AflIII A'CryG_T

Cuts at:      0     508     566     684 
   Size:        508      58     118

  Fragments arranged by size: 

                 508     118      58
 
ApoI r'AATT_y

Cuts at:      0     126     522     684 
   Size:        126     396     162

  Fragments arranged by size: 

                 396     162     126
 
BamHI G'GATC_C

Cuts at:      0      81     684 
   Size:         81     603
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     583     684 
   Size:        583     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     684 
   Size:        296     388
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     684 
   Size:        256     428
 
BsaAI yAC'GTr

Cuts at:      0     509     684 
   Size:        509     175
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     513     684 
   Size:        513     171
 
BstYI r'GATC_y

Cuts at:      0      81     611     684 
   Size:         81     530      73

  Fragments arranged by size: 

                 530      81      73
 
BtsI GCAGTG_nn'

Cuts at:      0     640     684 
   Size:        640      44
 
DraI TTT'AAA

Cuts at:      0     332     684 
   Size:        332     352
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     684 
   Size:        296     388
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     469     684 
   Size:        469     215
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     379     684 
   Size:        379     305
 
HindIII A'AGCT_T

Cuts at:      0     438     684 
   Size:        438     246
 
MfeI C'AATT_G

Cuts at:      0       9     684 
   Size:          9     675
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     684 
   Size:        253     431
 
MslI CAynn'nnrTG

Cuts at:      0     374     684 
   Size:        374     310
 
MspA1I CmG'CkG

Cuts at:      0     316     684 
   Size:        316     368
 
NspI r_CATG'y

Cuts at:      0     570     684 
   Size:        570     114
 
PciI A'CATG_T

Cuts at:      0     566     684 
   Size:        566     118
 
PmlI CAC'GTG

Cuts at:      0     509     684 
   Size:        509     175
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     554     586     684 
   Size:        554      32      98

  Fragments arranged by size: 

                 554      98      32
 
PvuII CAG'CTG

Cuts at:      0     316     684 
   Size:        316     368
 
ScaI AGT'ACT

Cuts at:      0     359     684 
   Size:        359     325
 
SmlI C'TyrA_G

Cuts at:      0     271     441     582     684 
   Size:        271     170     141     102

  Fragments arranged by size: 

                 271     170     141     102
 
StyI C'CwwG_G

Cuts at:      0     596     684 
   Size:        596      88
 
TatI w'GTAC_w

Cuts at:      0     357     684 
   Size:        357     327
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     684 
   Size:        104     580
 

 Enzymes that do cut:

    AflII   AflIII     ApoI    BamHI     BbsI     BpmI    BpuEI    BsaAI
    BstXI    BstYI     BtsI     DraI  Eco57MI    EcoNI     FalI  HindIII
     MfeI     MmeI     MslI   MspA1I     NspI     PciI     PmlI     PsrI
    PvuII     ScaI     SmlI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     KpnI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI