Restriction map for pF1KE0118
 (Linear) (Six-base) MAPSORT of: KIEE0118.seq  Check: 3410  from: 1  to: 585



KIEE0118.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     585 
   Size:        538      47
 
AflII C'TTAA_G

Cuts at:      0     432     585 
   Size:        432     153
 
ApoI r'AATT_y

Cuts at:      0     578     585 
   Size:        578       7
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     212     585 
   Size:        212     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     585 
   Size:        478     107
 
DraIII CAC_nnn'GTG

Cuts at:      0     316     585 
   Size:        316     269
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     326     585 
   Size:        326     259
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     538     585 
   Size:        478      60      47

  Fragments arranged by size: 

                 478      60      47
 
EcoO109I rG'GnC_Cy

Cuts at:      0     549     585 
   Size:        549      36
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     284     480     585 
   Size:        284     196     105

  Fragments arranged by size: 

                 284     196     105
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     171     203     585 
   Size:        171      32     382

  Fragments arranged by size: 

                 382     171      32
 
MfeI C'AATT_G

Cuts at:      0      38     585 
   Size:         38     547
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     585 
   Size:         96     489
 
PsiI TTA'TAA

Cuts at:      0     544     585 
   Size:        544      41
 
SmlI C'TyrA_G

Cuts at:      0     432     585 
   Size:        432     153
 
StyI C'CwwG_G

Cuts at:      0     516     585 
   Size:        516      69
 

 Enzymes that do cut:

     AcuI    AflII     ApoI    BciVI     BpmI   DraIII     DrdI  Eco57MI
 EcoO109I     FalI    Hin4I     MfeI     MmeI     PsiI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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