(Linear) (Six-base) MAPSORT of: KIEE0119.seq  Check: 6747  from: 1  to: 1023



KIEE0119.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:      0     144     600    1023 
   Size:        144     456     423

  Fragments arranged by size: 

                 456     423     144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296    1023 
   Size:        296     727
 
AflIII A'CryG_T

Cuts at:      0     474    1023 
   Size:        474     549
 
ApaLI G'TGCA_C

Cuts at:      0     431    1023 
   Size:        431     592
 
ApoI r'AATT_y

Cuts at:      0     159     167     319     333     504     614     643     848 
   Size:        159       8     152      14     171     110      29     205

Cuts at:    848    1023 
   Size:        175

  Fragments arranged by size: 

                 205     175     171     159     152     110      29      14
                   8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119    1023 
   Size:        119     904
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     913     947    1023 
   Size:        913      34      76

  Fragments arranged by size: 

                 913      76      34
 
BclI T'GATC_A

Cuts at:      0     516    1023 
   Size:        516     507
 
Bme1580I G_kGCm'C

Cuts at:      0     435    1023 
   Size:        435     588
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     967    1023 
   Size:        967      56
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     163    1023 
   Size:        163     860
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98    1023 
   Size:         98     925
 
BseYI C'CCAG_C

Cuts at:      0     818    1023 
   Size:        818     205
 
BsiHKAI G_wGCw'C

Cuts at:      0     435    1023 
   Size:        435     588
 
Bsp1286I G_dGCh'C

Cuts at:      0     435    1023 
   Size:        435     588
 
BsrBI CCG'CTC

Cuts at:      0     825    1023 
   Size:        825     198
 
BstYI r'GATC_y

Cuts at:      0     711    1023 
   Size:        711     312
 
BstZ17I GTA'TAC

Cuts at:      0     601    1023 
   Size:        601     422
 
DraI TTT'AAA

Cuts at:      0     420     705    1023 
   Size:        420     285     318

  Fragments arranged by size: 

                 420     318     285
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     296    1023 
   Size:         98     198     727

  Fragments arranged by size: 

                 727     198      98
 
EcoRI G'AATT_C

Cuts at:      0     167    1023 
   Size:        167     856
 
MfeI C'AATT_G

Cuts at:      0     219    1023 
   Size:        219     804
 
MslI CAynn'nnrTG

Cuts at:      0     479     887    1023 
   Size:        479     408     136

  Fragments arranged by size: 

                 479     408     136
 
NspI r_CATG'y

Cuts at:      0     478     571    1023 
   Size:        478      93     452

  Fragments arranged by size: 

                 478     452      93
 
PciI A'CATG_T

Cuts at:      0     474    1023 
   Size:        474     549
 
PfoI T'CCnGG_A

Cuts at:      0     342    1023 
   Size:        342     681
 
ScaI AGT'ACT

Cuts at:      0     309    1023 
   Size:        309     714
 
SfcI C'TryA_G

Cuts at:      0     997    1023 
   Size:        997      26
 
SphI G_CATG'C

Cuts at:      0     571    1023 
   Size:        571     452
 
SspI AAT'ATT

Cuts at:      0     234    1023 
   Size:        234     789
 
TatI w'GTAC_w

Cuts at:      0     307     378    1023 
   Size:        307      71     645

  Fragments arranged by size: 

                 645     307      71
 
XmnI GAAnn'nnTTC

Cuts at:      0     848    1023 
   Size:        848     175
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    ApaLI     ApoI     BbsI     BcgI     BclI
 Bme1580I     BmrI     BplI     BpmI    BseYI  BsiHKAI Bsp1286I    BsrBI
    BstYI  BstZ17I     DraI  Eco57MI    EcoRI     MfeI     MslI     NspI
     PciI     PfoI     ScaI     SfcI     SphI     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI