(Linear) (Six-base) MAPSORT of: KIEE0121.seq  Check: 2121  from: 1  to: 381



KIEE0121.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AvaI C'yCGr_G

Cuts at:      0      17      73     381 
   Size:         17      56     308

  Fragments arranged by size: 

                 308      56      17
 
BanI G'GyrC_C

Cuts at:      0      13     381 
   Size:         13     368
 
BanII G_rGCy'C

Cuts at:      0     135     381 
   Size:        135     246
 
Bme1580I G_kGCm'C

Cuts at:      0      18     381 
   Size:         18     363
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     381 
   Size:         37     344
 
BsiEI CG_ry'CG

Cuts at:      0       8     381 
   Size:          8     373
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     135     381 
   Size:         18     117     246

  Fragments arranged by size: 

                 246     117      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      97     195     381 
   Size:         97      98     186

  Fragments arranged by size: 

                 186      98      97
 
BsrFI r'CCGG_y

Cuts at:      0      10      41     381 
   Size:         10      31     340

  Fragments arranged by size: 

                 340      31      10
 
BsrGI T'GTAC_A

Cuts at:      0      92     381 
   Size:         92     289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     278     381 
   Size:        278     103
 
EaeI y'GGCC_r

Cuts at:      0       5     179     381 
   Size:          5     174     202

  Fragments arranged by size: 

                 202     174       5
 
EagI C'GGCC_G

Cuts at:      0       5     381 
   Size:          5     376
 
EcoO109I rG'GnC_Cy

Cuts at:      0     109     381 
   Size:        109     272
 
MscI TGG'CCA

Cuts at:      0     181     381 
   Size:        181     200
 
PpuMI rG'GwC_Cy

Cuts at:      0     109     381 
   Size:        109     272
 
PstI C_TGCA'G

Cuts at:      0     192     381 
   Size:        192     189
 
SfcI C'TryA_G

Cuts at:      0     188     230     381 
   Size:        188      42     151

  Fragments arranged by size: 

                 188     151      42
 
SgrAI Cr'CCGG_yG

Cuts at:      0      10     381 
   Size:         10     371
 
StuI AGG'CCT

Cuts at:      0      23     381 
   Size:         23     358
 
TatI w'GTAC_w

Cuts at:      0      92     381 
   Size:         92     289
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     143     381 
   Size:        143     238
 

 Enzymes that do cut:

     AvaI     BanI    BanII Bme1580I    BseRI    BsiEI Bsp1286I    BspMI
    BsrFI    BsrGI    BstXI     EaeI     EagI EcoO109I     MscI    PpuMI
     PstI     SfcI    SgrAI     StuI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI