Restriction map for
pF1KE0127
(Linear) (Six-base) MAPSORT of: KIEE0127.seq Check: 6454 from: 1 to: 1128
KIEE0127.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
Acc65I G'GTAC_C
Cuts at: 0 5 1128
Size: 5 1123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 70 550 656 1128
Size: 22 48 480 106 472
Fragments arranged by size:
480 472 106 48 22
AhdI GACnn_n'nnGTC
Cuts at: 0 326 1079 1128
Size: 326 753 49
Fragments arranged by size:
753 326 49
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1128
Size: 823 305
ApoI r'AATT_y
Cuts at: 0 862 1128
Size: 862 266
BanI G'GyrC_C
Cuts at: 0 5 122 852 1128
Size: 5 117 730 276
Fragments arranged by size:
730 276 117 5
BanII G_rGCy'C
Cuts at: 0 752 1128
Size: 752 376
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 218 252 1128
Size: 218 34 876
Fragments arranged by size:
876 218 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 279 1128
Size: 279 849
BclI T'GATC_A
Cuts at: 0 989 1128
Size: 989 139
BlpI GC'TnA_GC
Cuts at: 0 243 1128
Size: 243 885
BmtI G_CTAG'C
Cuts at: 0 712 1128
Size: 712 416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1128
Size: 666 462
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 578 1128
Size: 578 550
BsaI GGTCTCn'nnnn_
Cuts at: 0 224 331 1128
Size: 224 107 797
Fragments arranged by size:
797 224 107
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 862 1029 1128
Size: 71 791 167 99
Fragments arranged by size:
791 167 99 71
BsiHKAI G_wGCw'C
Cuts at: 0 602 1128
Size: 602 526
BsmI GAATG_Cn'
Cuts at: 0 462 568 758 1128
Size: 462 106 190 370
Fragments arranged by size:
462 370 190 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1095 1128
Size: 1095 33
Bsp1286I G_dGCh'C
Cuts at: 0 602 752 1128
Size: 602 150 376
Fragments arranged by size:
602 376 150
BsrBI CCG'CTC
Cuts at: 0 242 1128
Size: 242 886
BstXI CCAn_nnnn'nTGG
Cuts at: 0 330 937 1128
Size: 330 607 191
Fragments arranged by size:
607 330 191
Bsu36I CC'TnA_GG
Cuts at: 0 857 1128
Size: 857 271
BtgI C'CryG_G
Cuts at: 0 967 1128
Size: 967 161
BtsI GCAGTG_nn'
Cuts at: 0 234 692 805 1036 1128
Size: 234 458 113 231 92
Fragments arranged by size:
458 234 231 113 92
EaeI y'GGCC_r
Cuts at: 0 358 1128
Size: 358 770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 70 550 656 666 1128
Size: 22 48 480 106 10 462
Fragments arranged by size:
480 462 106 48 22 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 312 344 1065 1097 1128
Size: 312 32 721 32 31
Fragments arranged by size:
721 312 32 32 31
HincII GTy'rAC
Cuts at: 0 1023 1128
Size: 1023 105
HindIII A'AGCT_T
Cuts at: 0 96 1128
Size: 96 1032
KpnI G_GTAC'C
Cuts at: 0 9 1128
Size: 9 1119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1128
Size: 894 234
MscI TGG'CCA
Cuts at: 0 360 1128
Size: 360 768
MslI CAynn'nnrTG
Cuts at: 0 763 1047 1128
Size: 763 284 81
Fragments arranged by size:
763 284 81
NcoI C'CATG_G
Cuts at: 0 967 1128
Size: 967 161
NheI G'CTAG_C
Cuts at: 0 708 1128
Size: 708 420
SmlI C'TyrA_G
Cuts at: 0 593 1128
Size: 593 535
SspI AAT'ATT
Cuts at: 0 1070 1128
Size: 1070 58
StyI C'CwwG_G
Cuts at: 0 967 1128
Size: 967 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 1128
Size: 1 32 1095
Fragments arranged by size:
1095 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 711 1128
Size: 711 417
Enzymes that do cut:
Acc65I AcuI AhdI AlfI ApoI BanI BanII BcgI
BciVI BclI BlpI BmtI BpmI BpuEI BsaI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BsrBI BstXI Bsu36I BtgI
BtsI EaeI Eco57MI Hin4I HincII HindIII KpnI MmeI
MscI MslI NcoI NheI SmlI SspI StyI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MspA1I NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI