Restriction map for
pF1KE0131
(Linear) (Six-base) MAPSORT of: KIEE0131.seq Check: 7571 from: 1 to: 549
KIEE0131.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 549
Size: 170 379
AvaI C'yCGr_G
Cuts at: 0 131 549
Size: 131 418
BanI G'GyrC_C
Cuts at: 0 136 463 473 549
Size: 136 327 10 76
Fragments arranged by size:
327 136 76 10
BbeI G_GCGC'C
Cuts at: 0 467 477 549
Size: 467 10 72
Fragments arranged by size:
467 72 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 549
Size: 420 129
BclI T'GATC_A
Cuts at: 0 491 549
Size: 491 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 415 472 549
Size: 277 138 57 77
Fragments arranged by size:
277 138 77 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 478 549
Size: 478 71
BsaBI GATnn'nnATC
Cuts at: 0 287 549
Size: 287 262
BsaHI Gr'CG_yC
Cuts at: 0 69 438 464 474 549
Size: 69 369 26 10 75
Fragments arranged by size:
369 75 69 26 10
BseYI C'CCAG_C
Cuts at: 0 239 549
Size: 239 310
BsiHKAI G_wGCw'C
Cuts at: 0 92 549
Size: 92 457
BsmI GAATG_Cn'
Cuts at: 0 263 549
Size: 263 286
Bsp1286I G_dGCh'C
Cuts at: 0 92 549
Size: 92 457
BspHI T'CATG_A
Cuts at: 0 407 549
Size: 407 142
BsrDI GCAATG_nn'
Cuts at: 0 518 549
Size: 518 31
BsrFI r'CCGG_y
Cuts at: 0 434 549
Size: 434 115
BssHII G'CGCG_C
Cuts at: 0 444 549
Size: 444 105
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 549
Size: 231 318
BstYI r'GATC_y
Cuts at: 0 121 549
Size: 121 428
BtgI C'CryG_G
Cuts at: 0 276 549
Size: 276 273
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 480 549
Size: 480 69
BtsI GCAGTG_nn'
Cuts at: 0 365 549
Size: 365 184
DraIII CAC_nnn'GTG
Cuts at: 0 96 549
Size: 96 453
EaeI y'GGCC_r
Cuts at: 0 512 549
Size: 512 37
EarI CTCTTCn'nnn_
Cuts at: 0 374 549
Size: 374 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 43 549
Size: 43 506
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 170 277 415 472 549
Size: 170 107 138 57 77
Fragments arranged by size:
170 138 107 77 57
EcoO109I rG'GnC_Cy
Cuts at: 0 10 236 549
Size: 10 226 313
Fragments arranged by size:
313 226 10
HaeII r_GCGC'y
Cuts at: 0 467 477 549
Size: 467 10 72
Fragments arranged by size:
467 72 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 549
Size: 106 32 411
Fragments arranged by size:
411 106 32
HincII GTy'rAC
Cuts at: 0 432 549
Size: 432 117
KasI G'GCGC_C
Cuts at: 0 463 473 549
Size: 463 10 76
Fragments arranged by size:
463 76 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 549
Size: 140 409
MslI CAynn'nnrTG
Cuts at: 0 281 499 549
Size: 281 218 50
Fragments arranged by size:
281 218 50
MspA1I CmG'CkG
Cuts at: 0 243 420 549
Size: 243 177 129
Fragments arranged by size:
243 177 129
NarI GG'CG_CC
Cuts at: 0 464 474 549
Size: 464 10 75
Fragments arranged by size:
464 75 10
NcoI C'CATG_G
Cuts at: 0 276 549
Size: 276 273
PasI CC'CwG_GG
Cuts at: 0 346 549
Size: 346 203
PflMI CCAn_nnn'nTGG
Cuts at: 0 530 549
Size: 530 19
PfoI T'CCnGG_A
Cuts at: 0 293 549
Size: 293 256
PpuMI rG'GwC_Cy
Cuts at: 0 236 549
Size: 236 313
PvuII CAG'CTG
Cuts at: 0 243 420 549
Size: 243 177 129
Fragments arranged by size:
243 177 129
SanDI GG'GwC_CC
Cuts at: 0 236 549
Size: 236 313
SexAI A'CCwGG_T
Cuts at: 0 92 549
Size: 92 457
SfoI GGC'GCC
Cuts at: 0 465 475 549
Size: 465 10 74
Fragments arranged by size:
465 74 10
SmaI CCC'GGG
Cuts at: 0 133 549
Size: 133 416
StyI C'CwwG_G
Cuts at: 0 23 72 276 315 549
Size: 23 49 204 39 234
Fragments arranged by size:
234 204 49 39 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 116 549
Size: 116 433
TatI w'GTAC_w
Cuts at: 0 390 549
Size: 390 159
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 301 549
Size: 30 271 248
Fragments arranged by size:
271 248 30
XmaI C'CCGG_G
Cuts at: 0 131 549
Size: 131 418
Enzymes that do cut:
AcuI AvaI BanI BbeI BbsI BclI BpmI BsaI
BsaBI BsaHI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrDI
BsrFI BssHII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII
KasI MmeI MslI MspA1I NarI NcoI PasI PflMI
PfoI PpuMI PvuII SanDI SexAI SfoI SmaI StyI
TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaWI
BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspEI BspMI
BsrBI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmnI ZraI