Restriction map for
pF1KE0134
(Linear) (Six-base) MAPSORT of: KIEE0134.seq Check: 5231 from: 1 to: 642
KIEE0134.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 642
Size: 206 436
AgeI A'CCGG_T
Cuts at: 0 26 642
Size: 26 616
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 439 642
Size: 439 203
ApaI G_GGCC'C
Cuts at: 0 633 642
Size: 633 9
ApoI r'AATT_y
Cuts at: 0 206 538 642
Size: 206 332 104
Fragments arranged by size:
332 206 104
BanI G'GyrC_C
Cuts at: 0 349 642
Size: 349 293
BanII G_rGCy'C
Cuts at: 0 633 642
Size: 633 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 642
Size: 551 91
BglI GCCn_nnn'nGGC
Cuts at: 0 196 642
Size: 196 446
Bme1580I G_kGCm'C
Cuts at: 0 373 633 642
Size: 373 260 9
Fragments arranged by size:
373 260 9
BmgBI CAC'GTC
Cuts at: 0 285 642
Size: 285 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 596 642
Size: 596 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 642
Size: 214 428
BsaBI GATnn'nnATC
Cuts at: 0 496 642
Size: 496 146
BsaWI w'CCGG_w
Cuts at: 0 26 642
Size: 26 616
BseYI C'CCAG_C
Cuts at: 0 316 642
Size: 316 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 642
Size: 619 23
Bsp1286I G_dGCh'C
Cuts at: 0 373 633 642
Size: 373 260 9
Fragments arranged by size:
373 260 9
BsrFI r'CCGG_y
Cuts at: 0 26 642
Size: 26 616
BstYI r'GATC_y
Cuts at: 0 491 642
Size: 491 151
DraIII CAC_nnn'GTG
Cuts at: 0 156 642
Size: 156 486
EaeI y'GGCC_r
Cuts at: 0 147 377 393 480 642
Size: 147 230 16 87 162
Fragments arranged by size:
230 162 147 87 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 126 642
Size: 126 516
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 214 642
Size: 206 8 428
Fragments arranged by size:
428 206 8
EcoO109I rG'GnC_Cy
Cuts at: 0 450 630 642
Size: 450 180 12
Fragments arranged by size:
450 180 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 78 531 642
Size: 78 453 111
Fragments arranged by size:
453 111 78
FspI TGC'GCA
Cuts at: 0 457 642
Size: 457 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 642
Size: 163 32 447
Fragments arranged by size:
447 163 32
MscI TGG'CCA
Cuts at: 0 149 379 395 642
Size: 149 230 16 247
Fragments arranged by size:
247 230 149 16
MspA1I CmG'CkG
Cuts at: 0 177 642
Size: 177 465
NspI r_CATG'y
Cuts at: 0 345 442 642
Size: 345 97 200
Fragments arranged by size:
345 200 97
PasI CC'CwG_GG
Cuts at: 0 366 642
Size: 366 276
PspOMI G'GGCC_C
Cuts at: 0 629 642
Size: 629 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 31 63 642
Size: 31 32 579
Fragments arranged by size:
579 32 31
PstI C_TGCA'G
Cuts at: 0 428 642
Size: 428 214
PvuII CAG'CTG
Cuts at: 0 177 642
Size: 177 465
SfcI C'TryA_G
Cuts at: 0 424 642
Size: 424 218
SphI G_CATG'C
Cuts at: 0 345 642
Size: 345 297
TatI w'GTAC_w
Cuts at: 0 221 593 642
Size: 221 372 49
Fragments arranged by size:
372 221 49
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 642
Size: 222 420
Enzymes that do cut:
AcuI AgeI AlfI ApaI ApoI BanI BanII BbsI
BglI Bme1580I BmgBI BmrI BpmI BsaBI BsaWI BseYI
BsgI Bsp1286I BsrFI BstYI DraIII EaeI EciI Eco57MI
EcoO109I FalI FspI Hin4I MscI MspA1I NspI PasI
PspOMI PsrI PstI PvuII SfcI SphI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI