(Linear) (Six-base) MAPSORT of: KIEE0137.seq  Check: 9628  from: 1  to: 516



KIEE0137.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     178     516 
   Size:         98      80     338

  Fragments arranged by size: 

                 338      98      80
 
AflIII A'CryG_T

Cuts at:      0     381     516 
   Size:        381     135
 
ApaI G_GGCC'C

Cuts at:      0     177     516 
   Size:        177     339
 
BanI G'GyrC_C

Cuts at:      0     136     516 
   Size:        136     380
 
BanII G_rGCy'C

Cuts at:      0      61     177     516 
   Size:         61     116     339

  Fragments arranged by size: 

                 339     116      61
 
Bme1580I G_kGCm'C

Cuts at:      0     177     516 
   Size:        177     339
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     362     516 
   Size:        362     154
 
Bpu10I CC'TnA_GC

Cuts at:      0      62      68     516 
   Size:         62       6     448

  Fragments arranged by size: 

                 448      62       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433     516 
   Size:        433      83
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     177     516 
   Size:         61     116     339

  Fragments arranged by size: 

                 339     116      61
 
BspHI T'CATG_A

Cuts at:      0     494     516 
   Size:        494      22
 
BsrDI GCAATG_nn'

Cuts at:      0     173     516 
   Size:        173     343
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     516 
   Size:        396     120
 
BstYI r'GATC_y

Cuts at:      0      95     516 
   Size:         95     421
 
DraI TTT'AAA

Cuts at:      0      15     516 
   Size:         15     501
 
EaeI y'GGCC_r

Cuts at:      0     386     516 
   Size:        386     130
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     516 
   Size:         74     442
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     178     516 
   Size:         98      80     338

  Fragments arranged by size: 

                 338      98      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     173     174     516 
   Size:        173       1     342

  Fragments arranged by size: 

                 342     173       1
 
FspI TGC'GCA

Cuts at:      0     490     516 
   Size:        490      26
 
HindIII A'AGCT_T

Cuts at:      0     104     323     516 
   Size:        104     219     193

  Fragments arranged by size: 

                 219     193     104
 
MluI A'CGCG_T

Cuts at:      0     381     516 
   Size:        381     135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     516 
   Size:         32     484
 
MscI TGG'CCA

Cuts at:      0     388     516 
   Size:        388     128
 
PasI CC'CwG_GG

Cuts at:      0     177     516 
   Size:        177     339
 
PfoI T'CCnGG_A

Cuts at:      0      98     516 
   Size:         98     418
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     466     498     516 
   Size:        466      32      18

  Fragments arranged by size: 

                 466      32      18
 
PspOMI G'GGCC_C

Cuts at:      0     173     516 
   Size:        173     343
 
PstI C_TGCA'G

Cuts at:      0     428     516 
   Size:        428      88
 
SapI GCTCTTCn'nnn_

Cuts at:      0      74     516 
   Size:         74     442
 
SfcI C'TryA_G

Cuts at:      0     424     516 
   Size:        424      92
 
StyI C'CwwG_G

Cuts at:      0     389     516 
   Size:        389     127
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     388     516 
   Size:        388     128
 
XmnI GAAnn'nnTTC

Cuts at:      0     325     516 
   Size:        325     191
 

 Enzymes that do cut:

     AcuI   AflIII     ApaI     BanI    BanII Bme1580I     BmrI   Bpu10I
    BseRI Bsp1286I    BspHI    BsrDI    BstXI    BstYI     DraI     EaeI
     EarI  Eco57MI EcoO109I     FspI  HindIII     MluI     MmeI     MscI
     PasI     PfoI     PpiI   PspOMI     PstI     SapI     SfcI     StyI
    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I
   HincII     HpaI     KasI     KpnI     MfeI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI