Restriction map for
pF1KE0140
(Linear) (Six-base) MAPSORT of: KIEE0140.seq Check: 6886 from: 1 to: 855
KIEE0140.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 0 473 855
Size: 473 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 855
Size: 812 43
AflII C'TTAA_G
Cuts at: 0 492 855
Size: 492 363
AgeI A'CCGG_T
Cuts at: 0 344 855
Size: 344 511
AlwNI CAG_nnn'CTG
Cuts at: 0 517 855
Size: 517 338
ApoI r'AATT_y
Cuts at: 0 206 332 855
Size: 206 126 523
Fragments arranged by size:
523 206 126
AvaI C'yCGr_G
Cuts at: 0 59 855
Size: 59 796
BanI G'GyrC_C
Cuts at: 0 6 18 855
Size: 6 12 837
Fragments arranged by size:
837 12 6
BanII G_rGCy'C
Cuts at: 0 143 855
Size: 143 712
BglI GCCn_nnn'nGGC
Cuts at: 0 557 855
Size: 557 298
Bme1580I G_kGCm'C
Cuts at: 0 598 855
Size: 598 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 855
Size: 124 731
BsaWI w'CCGG_w
Cuts at: 0 344 855
Size: 344 511
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 855
Size: 121 734
BseYI C'CCAG_C
Cuts at: 0 263 410 591 855
Size: 263 147 181 264
Fragments arranged by size:
264 263 181 147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 855
Size: 350 505
BsiHKAI G_wGCw'C
Cuts at: 0 734 855
Size: 734 121
Bsp1286I G_dGCh'C
Cuts at: 0 143 598 734 855
Size: 143 455 136 121
Fragments arranged by size:
455 143 136 121
BsrFI r'CCGG_y
Cuts at: 0 15 344 855
Size: 15 329 511
Fragments arranged by size:
511 329 15
BssSI C'ACGA_G
Cuts at: 0 383 855
Size: 383 472
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 855
Size: 373 482
BstZ17I GTA'TAC
Cuts at: 0 474 855
Size: 474 381
BtgI C'CryG_G
Cuts at: 0 22 855
Size: 22 833
BtsI GCAGTG_nn'
Cuts at: 0 434 802 855
Size: 434 368 53
Fragments arranged by size:
434 368 53
DraIII CAC_nnn'GTG
Cuts at: 0 373 855
Size: 373 482
EarI CTCTTCn'nnn_
Cuts at: 0 695 855
Size: 695 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 855
Size: 812 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 605 855
Size: 605 250
EcoO109I rG'GnC_Cy
Cuts at: 0 216 855
Size: 216 639
HaeII r_GCGC'y
Cuts at: 0 411 855
Size: 411 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 855
Size: 397 458
MspA1I CmG'CkG
Cuts at: 0 24 855
Size: 24 831
PfoI T'CCnGG_A
Cuts at: 0 173 855
Size: 173 682
PpuMI rG'GwC_Cy
Cuts at: 0 216 855
Size: 216 639
PstI C_TGCA'G
Cuts at: 0 429 855
Size: 429 426
SacII CC_GC'GG
Cuts at: 0 25 855
Size: 25 830
ScaI AGT'ACT
Cuts at: 0 634 855
Size: 634 221
SfcI C'TryA_G
Cuts at: 0 36 245 425 602 855
Size: 36 209 180 177 253
Fragments arranged by size:
253 209 180 177 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 557 855
Size: 557 298
SmlI C'TyrA_G
Cuts at: 0 103 492 855
Size: 103 389 363
Fragments arranged by size:
389 363 103
SpeI A'CTAG_T
Cuts at: 0 460 855
Size: 460 395
StuI AGG'CCT
Cuts at: 0 551 611 855
Size: 551 60 244
Fragments arranged by size:
551 244 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 255 855
Size: 255 600
TatI w'GTAC_w
Cuts at: 0 632 855
Size: 632 223
XmnI GAAnn'nnTTC
Cuts at: 0 825 855
Size: 825 30
Enzymes that do cut:
AccI AcuI AflII AgeI AlwNI ApoI AvaI BanI
BanII BglI Bme1580I BpuEI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrFI BssSI BstAPI BstZ17I BtgI BtsI
DraIII EarI Eco57MI EcoNI EcoO109I HaeII MmeI MspA1I
PfoI PpuMI PstI SacII ScaI SfcI SfiI SmlI
SpeI StuI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstYI Bsu36I BtgZI ClaI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI SexAI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SspI StyI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI