Restriction map for
pF1KE0141
(Linear) (Six-base) MAPSORT of: KIEE0141.seq Check: 2929 from: 1 to: 672
KIEE0141.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 457 672
Size: 157 300 215
Fragments arranged by size:
300 215 157
AfeI AGC'GCT
Cuts at: 0 241 672
Size: 241 431
AgeI A'CCGG_T
Cuts at: 0 591 672
Size: 591 81
AvaI C'yCGr_G
Cuts at: 0 160 256 314 672
Size: 160 96 58 358
Fragments arranged by size:
358 160 96 58
BanII G_rGCy'C
Cuts at: 0 125 169 672
Size: 125 44 503
Fragments arranged by size:
503 125 44
Bme1580I G_kGCm'C
Cuts at: 0 407 672
Size: 407 265
BmgBI CAC'GTC
Cuts at: 0 90 321 672
Size: 90 231 351
Fragments arranged by size:
351 231 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 578 672
Size: 578 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 504 672
Size: 504 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 672
Size: 235 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 672
Size: 433 239
BsaAI yAC'GTr
Cuts at: 0 279 672
Size: 279 393
BsaHI Gr'CG_yC
Cuts at: 0 379 574 672
Size: 379 195 98
Fragments arranged by size:
379 195 98
BsaWI w'CCGG_w
Cuts at: 0 591 672
Size: 591 81
BseYI C'CCAG_C
Cuts at: 0 219 672
Size: 219 453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 343 672
Size: 187 156 329
Fragments arranged by size:
329 187 156
Bsp1286I G_dGCh'C
Cuts at: 0 125 169 407 672
Size: 125 44 238 265
Fragments arranged by size:
265 238 125 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 209 672
Size: 209 463
BsrFI r'CCGG_y
Cuts at: 0 591 672
Size: 591 81
BssSI C'ACGA_G
Cuts at: 0 208 672
Size: 208 464
BstAPI GCAn_nnn'nTGC
Cuts at: 0 242 672
Size: 242 430
BstYI r'GATC_y
Cuts at: 0 416 672
Size: 416 256
BtgI C'CryG_G
Cuts at: 0 146 269 672
Size: 146 123 403
Fragments arranged by size:
403 146 123
DraI TTT'AAA
Cuts at: 0 651 672
Size: 651 21
EaeI y'GGCC_r
Cuts at: 0 266 672
Size: 266 406
EarI CTCTTCn'nnn_
Cuts at: 0 361 672
Size: 361 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 235 457 672
Size: 157 78 222 215
Fragments arranged by size:
222 215 157 78
FspI TGC'GCA
Cuts at: 0 103 672
Size: 103 569
HaeII r_GCGC'y
Cuts at: 0 243 672
Size: 243 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 672
Size: 545 32 95
Fragments arranged by size:
545 95 32
HincII GTy'rAC
Cuts at: 0 456 672
Size: 456 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 672
Size: 567 105
MslI CAynn'nnrTG
Cuts at: 0 178 672
Size: 178 494
NcoI C'CATG_G
Cuts at: 0 269 672
Size: 269 403
PflMI CCAn_nnn'nTGG
Cuts at: 0 213 672
Size: 213 459
SapI GCTCTTCn'nnn_
Cuts at: 0 361 672
Size: 361 311
SmaI CCC'GGG
Cuts at: 0 316 672
Size: 316 356
SmlI C'TyrA_G
Cuts at: 0 448 672
Size: 448 224
StyI C'CwwG_G
Cuts at: 0 269 407 542 672
Size: 269 138 135 130
Fragments arranged by size:
269 138 135 130
Tth111I GACn'n_nGTC
Cuts at: 0 88 672
Size: 88 584
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 672
Size: 276 396
XmaI C'CCGG_G
Cuts at: 0 314 672
Size: 314 358
XmnI GAAnn'nnTTC
Cuts at: 0 370 672
Size: 370 302
Enzymes that do cut:
AcuI AfeI AgeI AvaI BanII Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaAI BsaHI BsaWI BseYI BsgI
Bsp1286I BspMI BsrFI BssSI BstAPI BstYI BtgI DraI
EaeI EarI Eco57MI FspI HaeII Hin4I HincII MmeI
MslI NcoI PflMI SapI SmaI SmlI StyI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI Bpu10I BsaI BsaBI BsaXI BseRI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI XbaI XhoI
ZraI