Restriction map for
pF1KE0143
(Linear) (Six-base) MAPSORT of: KIEE0143.seq Check: 7153 from: 1 to: 930
KIEE0143.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 162 930
Size: 162 768
ApaI G_GGCC'C
Cuts at: 0 139 153 930
Size: 139 14 777
Fragments arranged by size:
777 139 14
ApoI r'AATT_y
Cuts at: 0 300 930
Size: 300 630
AseI AT'TA_AT
Cuts at: 0 791 930
Size: 791 139
AvaI C'yCGr_G
Cuts at: 0 93 930
Size: 93 837
BamHI G'GATC_C
Cuts at: 0 37 930
Size: 37 893
BanI G'GyrC_C
Cuts at: 0 21 55 162 239 930
Size: 21 34 107 77 691
Fragments arranged by size:
691 107 77 34 21
BanII G_rGCy'C
Cuts at: 0 139 153 620 930
Size: 139 14 467 310
Fragments arranged by size:
467 310 139 14
BbeI G_GCGC'C
Cuts at: 0 59 930
Size: 59 871
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 877 930
Size: 220 657 53
Fragments arranged by size:
657 220 53
BglI GCCn_nnn'nGGC
Cuts at: 0 13 930
Size: 13 917
Bme1580I G_kGCm'C
Cuts at: 0 139 153 930
Size: 139 14 777
Fragments arranged by size:
777 139 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 930
Size: 265 665
BsaHI Gr'CG_yC
Cuts at: 0 56 930
Size: 56 874
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 265 930
Size: 262 3 665
Fragments arranged by size:
665 262 3
BseYI C'CCAG_C
Cuts at: 0 158 930
Size: 158 772
BsiEI CG_ry'CG
Cuts at: 0 8 173 930
Size: 8 165 757
Fragments arranged by size:
757 165 8
BsiHKAI G_wGCw'C
Cuts at: 0 620 930
Size: 620 310
BsmBI CGTCTCn'nnnn_
Cuts at: 0 698 930
Size: 698 232
Bsp1286I G_dGCh'C
Cuts at: 0 139 153 620 930
Size: 139 14 467 310
Fragments arranged by size:
467 310 139 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 174 930
Size: 174 756
BsrBI CCG'CTC
Cuts at: 0 133 930
Size: 133 797
BsrDI GCAATG_nn'
Cuts at: 0 773 930
Size: 773 157
BsrFI r'CCGG_y
Cuts at: 0 125 930
Size: 125 805
BstXI CCAn_nnnn'nTGG
Cuts at: 0 663 930
Size: 663 267
BstYI r'GATC_y
Cuts at: 0 37 930
Size: 37 893
Bsu36I CC'TnA_GG
Cuts at: 0 752 930
Size: 752 178
BtgI C'CryG_G
Cuts at: 0 8 930
Size: 8 922
BtsI GCAGTG_nn'
Cuts at: 0 84 668 930
Size: 84 584 262
Fragments arranged by size:
584 262 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 323 358 755 790 930
Size: 323 35 397 35 140
Fragments arranged by size:
397 323 140 35 35
DraI TTT'AAA
Cuts at: 0 897 930
Size: 897 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 881 930
Size: 881 49
EaeI y'GGCC_r
Cuts at: 0 5 68 170 608 827 930
Size: 5 63 102 438 219 103
Fragments arranged by size:
438 219 103 102 63 5
EagI C'GGCC_G
Cuts at: 0 5 170 930
Size: 5 165 760
Fragments arranged by size:
760 165 5
EcoICRI GAG'CTC
Cuts at: 0 618 930
Size: 618 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 930
Size: 265 665
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 563 610 930
Size: 247 316 47 320
Fragments arranged by size:
320 316 247 47
EcoO109I rG'GnC_Cy
Cuts at: 0 29 178 757 908 930
Size: 29 149 579 151 22
Fragments arranged by size:
579 151 149 29 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 892 904 930
Size: 892 12 26
Fragments arranged by size:
892 26 12
HaeII r_GCGC'y
Cuts at: 0 59 930
Size: 59 871
HindIII A'AGCT_T
Cuts at: 0 839 930
Size: 839 91
KasI G'GCGC_C
Cuts at: 0 55 930
Size: 55 875
KpnI G_GTAC'C
Cuts at: 0 166 930
Size: 166 764
MfeI C'AATT_G
Cuts at: 0 437 930
Size: 437 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 930
Size: 886 44
MscI TGG'CCA
Cuts at: 0 610 829 930
Size: 610 219 101
Fragments arranged by size:
610 219 101
MslI CAynn'nnrTG
Cuts at: 0 598 930
Size: 598 332
MspA1I CmG'CkG
Cuts at: 0 10 930
Size: 10 920
NaeI GCC'GGC
Cuts at: 0 127 930
Size: 127 803
NarI GG'CG_CC
Cuts at: 0 56 930
Size: 56 874
NgoMIV G'CCGG_C
Cuts at: 0 125 930
Size: 125 805
NotI GC'GGCC_GC
Cuts at: 0 5 930
Size: 5 925
NspI r_CATG'y
Cuts at: 0 49 930
Size: 49 881
PasI CC'CwG_GG
Cuts at: 0 153 400 930
Size: 153 247 530
Fragments arranged by size:
530 247 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 684 930
Size: 684 246
PpuMI rG'GwC_Cy
Cuts at: 0 178 757 930
Size: 178 579 173
Fragments arranged by size:
579 178 173
PsiI TTA'TAA
Cuts at: 0 322 930
Size: 322 608
PspOMI G'GGCC_C
Cuts at: 0 135 149 930
Size: 135 14 781
Fragments arranged by size:
781 135 14
SacI G_AGCT'C
Cuts at: 0 620 930
Size: 620 310
SacII CC_GC'GG
Cuts at: 0 11 930
Size: 11 919
SanDI GG'GwC_CC
Cuts at: 0 178 930
Size: 178 752
SfcI C'TryA_G
Cuts at: 0 198 714 930
Size: 198 516 216
Fragments arranged by size:
516 216 198
SfoI GGC'GCC
Cuts at: 0 57 930
Size: 57 873
SmaI CCC'GGG
Cuts at: 0 95 930
Size: 95 835
SphI G_CATG'C
Cuts at: 0 49 930
Size: 49 881
StuI AGG'CCT
Cuts at: 0 724 930
Size: 724 206
StyI C'CwwG_G
Cuts at: 0 32 611 725 930
Size: 32 579 114 205
Fragments arranged by size:
579 205 114 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 254 286 396 428 930
Size: 254 32 110 32 502
Fragments arranged by size:
502 254 110 32 32
XmaI C'CCGG_G
Cuts at: 0 93 930
Size: 93 837
XmnI GAAnn'nnTTC
Cuts at: 0 884 930
Size: 884 46
Enzymes that do cut:
Acc65I ApaI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BglI Bme1580I BpmI BsaHI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraI DrdI
EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PasI PflMI PpuMI
PsiI PspOMI SacI SacII SanDI SfcI SfoI SmaI
SphI StuI StyI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI DraIII EarI EciI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HpaI MluI NcoI
NdeI NheI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PstI PvuI PvuII
RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI ZraI