(Linear) (Six-base) MAPSORT of: KIEE0145.seq  Check: 2443  from: 1  to: 375



KIEE0145.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AflIII A'CryG_T

Cuts at:      0     266     375 
   Size:        266     109
 
AhdI GACnn_n'nnGTC

Cuts at:      0     177     375 
   Size:        177     198
 
BanII G_rGCy'C

Cuts at:      0     105     375 
   Size:        105     270
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      48     375 
   Size:         48     327
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     108     142     375 
   Size:        108      34     233

  Fragments arranged by size: 

                 233     108      34
 
BsaAI yAC'GTr

Cuts at:      0     267     375 
   Size:        267     108
 
BsaBI GATnn'nnATC

Cuts at:      0      76     375 
   Size:         76     299
 
BsaHI Gr'CG_yC

Cuts at:      0      53     375 
   Size:         53     322
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58     375 
   Size:         28      30     317

  Fragments arranged by size: 

                 317      30      28
 
BseYI C'CCAG_C

Cuts at:      0     299     375 
   Size:        299      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     105     375 
   Size:        105     270
 
BsrBI CCG'CTC

Cuts at:      0      95     375 
   Size:         95     280
 
BstEII G'GTnAC_C

Cuts at:      0     351     375 
   Size:        351      24
 
BtgI C'CryG_G

Cuts at:      0     281     375 
   Size:        281      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     349     375 
   Size:        349      26
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      25     375 
   Size:         25     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     163     195     375 
   Size:         28      32     103      32     180

  Fragments arranged by size: 

                 180     103      32      32      28
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     375 
   Size:         71     304
 
NcoI C'CATG_G

Cuts at:      0     281     375 
   Size:        281      94
 
NspI r_CATG'y

Cuts at:      0     192     375 
   Size:        192     183
 
PmlI CAC'GTG

Cuts at:      0     267     375 
   Size:        267     108
 
PstI C_TGCA'G

Cuts at:      0     114     375 
   Size:        114     261
 
SfcI C'TryA_G

Cuts at:      0     110     375 
   Size:        110     265
 
StyI C'CwwG_G

Cuts at:      0     281     375 
   Size:        281      94
 
Tth111I GACn'n_nGTC

Cuts at:      0      52     375 
   Size:         52     323
 

 Enzymes that do cut:

   AflIII     AhdI    BanII     BbsI     BcgI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI Bsp1286I    BsrBI   BstEII     BtgI     DrdI     EciI
    Hin4I     MmeI     NcoI     NspI     PmlI     PstI     SfcI     StyI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI