Restriction map for
pF1KE0150
(Linear) (Six-base) MAPSORT of: KIEE0150.seq Check: 938 from: 1 to: 945
KIEE0150.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 135 945
Size: 135 810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 945
Size: 222 723
ApoI r'AATT_y
Cuts at: 0 916 945
Size: 916 29
AvaI C'yCGr_G
Cuts at: 0 175 945
Size: 175 770
BanI G'GyrC_C
Cuts at: 0 114 847 945
Size: 114 733 98
Fragments arranged by size:
733 114 98
BbeI G_GCGC'C
Cuts at: 0 118 851 945
Size: 118 733 94
Fragments arranged by size:
733 118 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 747 945
Size: 747 198
BfrBI ATG'CAT
Cuts at: 0 3 945
Size: 3 942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 384 945
Size: 359 25 561
Fragments arranged by size:
561 359 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 945
Size: 671 274
BsaHI Gr'CG_yC
Cuts at: 0 47 115 154 848 945
Size: 47 68 39 694 97
Fragments arranged by size:
694 97 68 47 39
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 499 945
Size: 499 446
BseYI C'CCAG_C
Cuts at: 0 403 945
Size: 403 542
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 945
Size: 169 776
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 135 945
Size: 135 810
BsrBI CCG'CTC
Cuts at: 0 126 945
Size: 126 819
BsrDI GCAATG_nn'
Cuts at: 0 680 945
Size: 680 265
BsrFI r'CCGG_y
Cuts at: 0 76 945
Size: 76 869
BstXI CCAn_nnnn'nTGG
Cuts at: 0 565 945
Size: 565 380
Bsu36I CC'TnA_GG
Cuts at: 0 482 945
Size: 482 463
EaeI y'GGCC_r
Cuts at: 0 74 945
Size: 74 871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 945
Size: 95 850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 359 384 945
Size: 222 137 25 561
Fragments arranged by size:
561 222 137 25
EcoO109I rG'GnC_Cy
Cuts at: 0 66 478 945
Size: 66 412 467
Fragments arranged by size:
467 412 66
EcoRI G'AATT_C
Cuts at: 0 916 945
Size: 916 29
HaeII r_GCGC'y
Cuts at: 0 118 851 945
Size: 118 733 94
Fragments arranged by size:
733 118 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 446 478 945
Size: 37 32 377 32 467
Fragments arranged by size:
467 377 37 32 32
KasI G'GCGC_C
Cuts at: 0 114 847 945
Size: 114 733 98
Fragments arranged by size:
733 114 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 945
Size: 307 638
MslI CAynn'nnrTG
Cuts at: 0 417 589 945
Size: 417 172 356
Fragments arranged by size:
417 356 172
MspA1I CmG'CkG
Cuts at: 0 94 109 945
Size: 94 15 836
Fragments arranged by size:
836 94 15
NaeI GCC'GGC
Cuts at: 0 78 945
Size: 78 867
NarI GG'CG_CC
Cuts at: 0 115 848 945
Size: 115 733 97
Fragments arranged by size:
733 115 97
NgoMIV G'CCGG_C
Cuts at: 0 76 945
Size: 76 869
NruI TCG'CGA
Cuts at: 0 224 945
Size: 224 721
NsiI A_TGCA'T
Cuts at: 0 5 945
Size: 5 940
NspI r_CATG'y
Cuts at: 0 704 945
Size: 704 241
PpuMI rG'GwC_Cy
Cuts at: 0 66 478 945
Size: 66 412 467
Fragments arranged by size:
467 412 66
PstI C_TGCA'G
Cuts at: 0 146 945
Size: 146 799
PvuII CAG'CTG
Cuts at: 0 94 945
Size: 94 851
SfcI C'TryA_G
Cuts at: 0 142 945
Size: 142 803
SfoI GGC'GCC
Cuts at: 0 116 849 945
Size: 116 733 96
Fragments arranged by size:
733 116 96
SmlI C'TyrA_G
Cuts at: 0 686 945
Size: 686 259
StyI C'CwwG_G
Cuts at: 0 558 945
Size: 558 387
TatI w'GTAC_w
Cuts at: 0 576 945
Size: 576 369
XbaI T'CTAG_A
Cuts at: 0 435 945
Size: 435 510
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 945
Size: 108 837
XmnI GAAnn'nnTTC
Cuts at: 0 714 831 920 945
Size: 714 117 89 25
Fragments arranged by size:
714 117 89 25
Enzymes that do cut:
AarI AcuI ApoI AvaI BanI BbeI BbsI BfrBI
BpmI BpuEI BsaHI BseRI BseYI BsgI BspMI BsrBI
BsrDI BsrFI BstXI Bsu36I EaeI EciI Eco57MI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NruI NsiI NspI PpuMI PstI PvuII
SfcI SfoI SmlI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NotI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI