(Linear) (Six-base) MAPSORT of: KIEE0151.seq  Check: 6550  from: 1  to: 357



KIEE0151.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     357 
   Size:        323      34
 
ApoI r'AATT_y

Cuts at:      0     329     340     357 
   Size:        329      11      17

  Fragments arranged by size: 

                 329      17      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     239     357 
   Size:        239     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     357 
   Size:         79     278
 
BsiEI CG_ry'CG

Cuts at:      0     155     357 
   Size:        155     202
 
BtgI C'CryG_G

Cuts at:      0      82     357 
   Size:         82     275
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     157     357 
   Size:        157     200
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      27     159     357 
   Size:         27     132     198

  Fragments arranged by size: 

                 198     132      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     357 
   Size:        323      34
 
MspA1I CmG'CkG

Cuts at:      0      84     357 
   Size:         84     273
 
NdeI CA'TA_TG

Cuts at:      0     321     357 
   Size:        321      36
 
NruI TCG'CGA

Cuts at:      0     157     357 
   Size:        157     200
 
PvuI CG_AT'CG

Cuts at:      0     155     357 
   Size:        155     202
 
SacII CC_GC'GG

Cuts at:      0      85     357 
   Size:         85     272
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      91     357 
   Size:         91     266
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BseRI    BsiEI     BtgI    BtgZI     EciI
  Eco57MI   MspA1I     NdeI     NruI     PvuI    SacII    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI