(Linear) (Six-base) MAPSORT of: KIEE0153.seq  Check: 5374  from: 1  to: 1038



KIEE0153.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

Acc65I G'GTAC_C

Cuts at:      0     710    1038 
   Size:        710     328
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    1038 
   Size:        170     868
 
AlwNI CAG_nnn'CTG

Cuts at:      0     736    1038 
   Size:        736     302
 
ApoI r'AATT_y

Cuts at:      0      79     300     412     749    1038 
   Size:         79     221     112     337     289

  Fragments arranged by size: 

                 337     289     221     112      79
 
BanI G'GyrC_C

Cuts at:      0     710    1038 
   Size:        710     328
 
BanII G_rGCy'C

Cuts at:      0     722    1038 
   Size:        722     316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     336    1038 
   Size:         84     252     702

  Fragments arranged by size: 

                 702     252      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     727    1038 
   Size:        727     311
 
BsaBI GATnn'nnATC

Cuts at:      0     158    1038 
   Size:        158     880
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     464     494    1038 
   Size:        464      30     544

  Fragments arranged by size: 

                 544     464      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     799    1003    1038 
   Size:        799     204      35

  Fragments arranged by size: 

                 799     204      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692     853    1038 
   Size:        692     161     185

  Fragments arranged by size: 

                 692     185     161
 
BsiHKAI G_wGCw'C

Cuts at:      0     722    1038 
   Size:        722     316
 
Bsp1286I G_dGCh'C

Cuts at:      0     722    1038 
   Size:        722     316
 
BstBI TT'CG_AA

Cuts at:      0     111    1038 
   Size:        111     927
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    1038 
   Size:        594     444
 
BstYI r'GATC_y

Cuts at:      0     885    1038 
   Size:        885     153
 
BtsI GCAGTG_nn'

Cuts at:      0      52    1038 
   Size:         52     986
 
EaeI y'GGCC_r

Cuts at:      0     927    1038 
   Size:        927     111
 
EarI CTCTTCn'nnn_

Cuts at:      0     955    1038 
   Size:        955      83
 
EcoICRI GAG'CTC

Cuts at:      0     720    1038 
   Size:        720     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     170     336    1038 
   Size:         84      86     166     702

  Fragments arranged by size: 

                 702     166      86      84
 
EcoRV GAT'ATC

Cuts at:      0     255    1038 
   Size:        255     783
 
HindIII A'AGCT_T

Cuts at:      0     136     797    1038 
   Size:        136     661     241

  Fragments arranged by size: 

                 661     241     136
 
KpnI G_GTAC'C

Cuts at:      0     714    1038 
   Size:        714     324
 
MscI TGG'CCA

Cuts at:      0     929    1038 
   Size:        929     109
 
PsiI TTA'TAA

Cuts at:      0     134    1038 
   Size:        134     904
 
PstI C_TGCA'G

Cuts at:      0     492    1038 
   Size:        492     546
 
SacI G_AGCT'C

Cuts at:      0     722    1038 
   Size:        722     316
 
SfcI C'TryA_G

Cuts at:      0     488    1038 
   Size:        488     550
 
SmlI C'TyrA_G

Cuts at:      0     742    1038 
   Size:        742     296
 
StuI AGG'CCT

Cuts at:      0     296    1038 
   Size:        296     742
 
StyI C'CwwG_G

Cuts at:      0     225    1038 
   Size:        225     813
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     162     194    1038 
   Size:        162      32     844

  Fragments arranged by size: 

                 844     162      32
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BpmI    BpuEI
    BsaBI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BstBI    BstXI
    BstYI     BtsI     EaeI     EarI  EcoICRI  Eco57MI    EcoRV  HindIII
     KpnI     MscI     PsiI     PstI     SacI     SfcI     SmlI     StuI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI