Restriction map for
pF1KE0154
(Linear) (Six-base) MAPSORT of: KIEE0154.seq Check: 8825 from: 1 to: 894
KIEE0154.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 145 894
Size: 145 749
Acc65I G'GTAC_C
Cuts at: 0 65 894
Size: 65 829
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 894
Size: 227 667
ApoI r'AATT_y
Cuts at: 0 180 667 894
Size: 180 487 227
Fragments arranged by size:
487 227 180
BanI G'GyrC_C
Cuts at: 0 7 65 894
Size: 7 58 829
Fragments arranged by size:
829 58 7
BbeI G_GCGC'C
Cuts at: 0 11 894
Size: 11 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 893 894
Size: 893 1
BbvCI CC'TCA_GC
Cuts at: 0 517 894
Size: 517 377
BciVI GTATCCnnnnn_n'
Cuts at: 0 464 497 894
Size: 464 33 397
Fragments arranged by size:
464 397 33
Bme1580I G_kGCm'C
Cuts at: 0 576 827 894
Size: 576 251 67
Fragments arranged by size:
576 251 67
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 894
Size: 387 507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 387 894
Size: 223 164 507
Fragments arranged by size:
507 223 164
Bpu10I CC'TnA_GC
Cuts at: 0 517 894
Size: 517 377
BsaAI yAC'GTr
Cuts at: 0 111 384 894
Size: 111 273 510
Fragments arranged by size:
510 273 111
BsaHI Gr'CG_yC
Cuts at: 0 8 894
Size: 8 886
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 894
Size: 373 30 491
Fragments arranged by size:
491 373 30
BseYI C'CCAG_C
Cuts at: 0 152 490 894
Size: 152 338 404
Fragments arranged by size:
404 338 152
BsiEI CG_ry'CG
Cuts at: 0 177 894
Size: 177 717
BsiHKAI G_wGCw'C
Cuts at: 0 237 894
Size: 237 657
BsmBI CGTCTCn'nnnn_
Cuts at: 0 54 894
Size: 54 840
Bsp1286I G_dGCh'C
Cuts at: 0 237 576 827 894
Size: 237 339 251 67
Fragments arranged by size:
339 251 237 67
BspHI T'CATG_A
Cuts at: 0 374 894
Size: 374 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 894
Size: 145 749
BtgI C'CryG_G
Cuts at: 0 86 894
Size: 86 808
BtsI GCAGTG_nn'
Cuts at: 0 147 803 831 894
Size: 147 656 28 63
Fragments arranged by size:
656 147 63 28
DraI TTT'AAA
Cuts at: 0 354 506 894
Size: 354 152 388
Fragments arranged by size:
388 354 152
DrdI GACnn_nn'nnGTC
Cuts at: 0 475 894
Size: 475 419
EaeI y'GGCC_r
Cuts at: 0 38 174 894
Size: 38 136 720
Fragments arranged by size:
720 136 38
EagI C'GGCC_G
Cuts at: 0 174 894
Size: 174 720
EarI CTCTTCn'nnn_
Cuts at: 0 354 468 894
Size: 354 114 426
Fragments arranged by size:
426 354 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 387 894
Size: 223 164 507
Fragments arranged by size:
507 223 164
EcoRI G'AATT_C
Cuts at: 0 667 894
Size: 667 227
EcoRV GAT'ATC
Cuts at: 0 840 894
Size: 840 54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 225 894
Size: 225 669
FspI TGC'GCA
Cuts at: 0 260 894
Size: 260 634
HaeII r_GCGC'y
Cuts at: 0 11 894
Size: 11 883
KasI G'GCGC_C
Cuts at: 0 7 894
Size: 7 887
KpnI G_GTAC'C
Cuts at: 0 69 894
Size: 69 825
MfeI C'AATT_G
Cuts at: 0 345 894
Size: 345 549
MscI TGG'CCA
Cuts at: 0 40 894
Size: 40 854
MspA1I CmG'CkG
Cuts at: 0 243 521 894
Size: 243 278 373
Fragments arranged by size:
373 278 243
NarI GG'CG_CC
Cuts at: 0 8 894
Size: 8 886
NcoI C'CATG_G
Cuts at: 0 86 894
Size: 86 808
PmlI CAC'GTG
Cuts at: 0 384 894
Size: 384 510
PsiI TTA'TAA
Cuts at: 0 557 894
Size: 557 337
PvuII CAG'CTG
Cuts at: 0 243 521 894
Size: 243 278 373
Fragments arranged by size:
373 278 243
SapI GCTCTTCn'nnn_
Cuts at: 0 354 894
Size: 354 540
SfoI GGC'GCC
Cuts at: 0 9 894
Size: 9 885
StyI C'CwwG_G
Cuts at: 0 35 86 612 894
Size: 35 51 526 282
Fragments arranged by size:
526 282 51 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 894
Size: 144 750
TatI w'GTAC_w
Cuts at: 0 317 603 894
Size: 317 286 291
Fragments arranged by size:
317 291 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 464 496 894
Size: 373 32 59 32 398
Fragments arranged by size:
398 373 59 32 32
XbaI T'CTAG_A
Cuts at: 0 879 894
Size: 879 15
Enzymes that do cut:
AarI Acc65I AlfI ApoI BanI BbeI BbsI BbvCI
BciVI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaXI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BtgI
BtsI DraI DrdI EaeI EagI EarI Eco57MI EcoRI
EcoRV FalI FspI HaeII KasI KpnI MfeI MscI
MspA1I NarI NcoI PmlI PsiI PvuII SapI SfoI
StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI
BsaI BsaBI BsaWI BseRI BsgI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII EciI EcoICRI EcoNI EcoO109I FseI FspAI Hin4I
HincII HindIII HpaI MluI MmeI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XcmI XhoI XmaI XmnI ZraI