(Linear) (Six-base) MAPSORT of: KIEE0156.seq Check: 6222 from: 1 to: 783 KIEE0156.orf With 182 enzymes: SgfI * April 24, 2009 13:11 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 746 783 Size: 746 37 AflIII A'CryG_T Cuts at: 0 224 341 783 Size: 224 117 442 Fragments arranged by size: 442 224 117 AvaI C'yCGr_G Cuts at: 0 194 551 783 Size: 194 357 232 Fragments arranged by size: 357 232 194 BamHI G'GATC_C Cuts at: 0 572 783 Size: 572 211 BanI G'GyrC_C Cuts at: 0 248 388 783 Size: 248 140 395 Fragments arranged by size: 395 248 140 BciVI GTATCCnnnnn_n' Cuts at: 0 730 783 Size: 730 53 BglI GCCn_nnn'nGGC Cuts at: 0 217 783 Size: 217 566 Bme1580I G_kGCm'C Cuts at: 0 391 783 Size: 391 392 BmgBI CAC'GTC Cuts at: 0 225 783 Size: 225 558 BmrI ACTGGGnnnn_n' Cuts at: 0 366 783 Size: 366 417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 783 Size: 542 241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 783 Size: 367 416 BsaHI Gr'CG_yC Cuts at: 0 4 783 Size: 4 779 BseYI C'CCAG_C Cuts at: 0 162 783 Size: 162 621 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 322 783 Size: 286 36 461 Fragments arranged by size: 461 286 36 BsiEI CG_ry'CG Cuts at: 0 72 783 Size: 72 711 BsmI GAATG_Cn' Cuts at: 0 712 783 Size: 712 71 BsmBI CGTCTCn'nnnn_ Cuts at: 0 11 142 783 Size: 11 131 641 Fragments arranged by size: 641 131 11 Bsp1286I G_dGCh'C Cuts at: 0 391 783 Size: 391 392 BsrBI CCG'CTC Cuts at: 0 29 62 76 783 Size: 29 33 14 707 Fragments arranged by size: 707 33 29 14 BsrDI GCAATG_nn' Cuts at: 0 602 783 Size: 602 181 BstYI r'GATC_y Cuts at: 0 572 783 Size: 572 211 Bsu36I CC'TnA_GG Cuts at: 0 689 783 Size: 689 94 EaeI y'GGCC_r Cuts at: 0 69 92 783 Size: 69 23 691 Fragments arranged by size: 691 69 23 EagI C'GGCC_G Cuts at: 0 69 783 Size: 69 714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 746 783 Size: 542 204 37 Fragments arranged by size: 542 204 37 EcoO109I rG'GnC_Cy Cuts at: 0 684 749 783 Size: 684 65 34 Fragments arranged by size: 684 65 34 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 408 783 Size: 408 375 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 557 589 783 Size: 557 32 194 Fragments arranged by size: 557 194 32 HincII GTy'rAC Cuts at: 0 378 783 Size: 378 405 MspA1I CmG'CkG Cuts at: 0 139 578 783 Size: 139 439 205 Fragments arranged by size: 439 205 139 NspI r_CATG'y Cuts at: 0 345 783 Size: 345 438 PasI CC'CwG_GG Cuts at: 0 595 783 Size: 595 188 PciI A'CATG_T Cuts at: 0 341 783 Size: 341 442 PpuMI rG'GwC_Cy Cuts at: 0 684 749 783 Size: 684 65 34 Fragments arranged by size: 684 65 34 PstI C_TGCA'G Cuts at: 0 414 783 Size: 414 369 SanDI GG'GwC_CC Cuts at: 0 684 783 Size: 684 99 SfcI C'TryA_G Cuts at: 0 410 783 Size: 410 373 SmaI CCC'GGG Cuts at: 0 553 783 Size: 553 230 SmlI C'TyrA_G Cuts at: 0 382 783 Size: 382 401 StuI AGG'CCT Cuts at: 0 271 783 Size: 271 512 StyI C'CwwG_G Cuts at: 0 41 515 783 Size: 41 474 268 Fragments arranged by size: 474 268 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 334 627 783 Size: 334 293 156 Fragments arranged by size: 334 293 156 Tth111I GACn'n_nGTC Cuts at: 0 364 783 Size: 364 419 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 252 783 Size: 252 531 XmaI C'CCGG_G Cuts at: 0 551 783 Size: 551 232 Enzymes that do cut: AcuI AflIII AvaI BamHI BanI BciVI BglI Bme1580I BmgBI BmrI BpmI BpuEI BsaHI BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BsrBI BsrDI BstYI Bsu36I EaeI EagI Eco57MI EcoO109I FalI Hin4I HincII MspA1I NspI PasI PciI PpuMI PstI SanDI SfcI SmaI SmlI StuI StyI TaqII Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI BsiWI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XhoI XmnI ZraI