Restriction map for pF1KE0156
 (Linear) (Six-base) MAPSORT of: KIEE0156.seq  Check: 6222  from: 1  to: 783



KIEE0156.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     746     783 
   Size:        746      37
 
AflIII A'CryG_T

Cuts at:      0     224     341     783 
   Size:        224     117     442

  Fragments arranged by size: 

                 442     224     117
 
AvaI C'yCGr_G

Cuts at:      0     194     551     783 
   Size:        194     357     232

  Fragments arranged by size: 

                 357     232     194
 
BamHI G'GATC_C

Cuts at:      0     572     783 
   Size:        572     211
 
BanI G'GyrC_C

Cuts at:      0     248     388     783 
   Size:        248     140     395

  Fragments arranged by size: 

                 395     248     140
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     730     783 
   Size:        730      53
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217     783 
   Size:        217     566
 
Bme1580I G_kGCm'C

Cuts at:      0     391     783 
   Size:        391     392
 
BmgBI CAC'GTC

Cuts at:      0     225     783 
   Size:        225     558
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     366     783 
   Size:        366     417
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     783 
   Size:        542     241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     783 
   Size:        367     416
 
BsaHI Gr'CG_yC

Cuts at:      0       4     783 
   Size:          4     779
 
BseYI C'CCAG_C

Cuts at:      0     162     783 
   Size:        162     621
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     322     783 
   Size:        286      36     461

  Fragments arranged by size: 

                 461     286      36
 
BsiEI CG_ry'CG

Cuts at:      0      72     783 
   Size:         72     711
 
BsmI GAATG_Cn'

Cuts at:      0     712     783 
   Size:        712      71
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      11     142     783 
   Size:         11     131     641

  Fragments arranged by size: 

                 641     131      11
 
Bsp1286I G_dGCh'C

Cuts at:      0     391     783 
   Size:        391     392
 
BsrBI CCG'CTC

Cuts at:      0      29      62      76     783 
   Size:         29      33      14     707

  Fragments arranged by size: 

                 707      33      29      14
 
BsrDI GCAATG_nn'

Cuts at:      0     602     783 
   Size:        602     181
 
BstYI r'GATC_y

Cuts at:      0     572     783 
   Size:        572     211
 
Bsu36I CC'TnA_GG

Cuts at:      0     689     783 
   Size:        689      94
 
EaeI y'GGCC_r

Cuts at:      0      69      92     783 
   Size:         69      23     691

  Fragments arranged by size: 

                 691      69      23
 
EagI C'GGCC_G

Cuts at:      0      69     783 
   Size:         69     714
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     542     746     783 
   Size:        542     204      37

  Fragments arranged by size: 

                 542     204      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0     684     749     783 
   Size:        684      65      34

  Fragments arranged by size: 

                 684      65      34
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     408     783 
   Size:        408     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     557     589     783 
   Size:        557      32     194

  Fragments arranged by size: 

                 557     194      32
 
HincII GTy'rAC

Cuts at:      0     378     783 
   Size:        378     405
 
MspA1I CmG'CkG

Cuts at:      0     139     578     783 
   Size:        139     439     205

  Fragments arranged by size: 

                 439     205     139
 
NspI r_CATG'y

Cuts at:      0     345     783 
   Size:        345     438
 
PasI CC'CwG_GG

Cuts at:      0     595     783 
   Size:        595     188
 
PciI A'CATG_T

Cuts at:      0     341     783 
   Size:        341     442
 
PpuMI rG'GwC_Cy

Cuts at:      0     684     749     783 
   Size:        684      65      34

  Fragments arranged by size: 

                 684      65      34
 
PstI C_TGCA'G

Cuts at:      0     414     783 
   Size:        414     369
 
SanDI GG'GwC_CC

Cuts at:      0     684     783 
   Size:        684      99
 
SfcI C'TryA_G

Cuts at:      0     410     783 
   Size:        410     373
 
SmaI CCC'GGG

Cuts at:      0     553     783 
   Size:        553     230
 
SmlI C'TyrA_G

Cuts at:      0     382     783 
   Size:        382     401
 
StuI AGG'CCT

Cuts at:      0     271     783 
   Size:        271     512
 
StyI C'CwwG_G

Cuts at:      0      41     515     783 
   Size:         41     474     268

  Fragments arranged by size: 

                 474     268      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     334     627     783 
   Size:        334     293     156

  Fragments arranged by size: 

                 334     293     156
 
Tth111I GACn'n_nGTC

Cuts at:      0     364     783 
   Size:        364     419
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     252     783 
   Size:        252     531
 
XmaI C'CCGG_G

Cuts at:      0     551     783 
   Size:        551     232
 

 Enzymes that do cut:

     AcuI   AflIII     AvaI    BamHI     BanI    BciVI     BglI Bme1580I
    BmgBI     BmrI     BpmI    BpuEI    BsaHI    BseYI     BsgI    BsiEI
     BsmI    BsmBI Bsp1286I    BsrBI    BsrDI    BstYI   Bsu36I     EaeI
     EagI  Eco57MI EcoO109I     FalI    Hin4I   HincII   MspA1I     NspI
     PasI     PciI    PpuMI     PstI    SanDI     SfcI     SmaI     SmlI
     StuI     StyI    TaqII  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI  BsiHKAI
    BsiWI    BspEI    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI     XbaI     XhoI     XmnI     ZraI
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