(Linear) (Six-base) MAPSORT of: KIEE0160.seq  Check: 8399  from: 1  to: 279



KIEE0160.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     279 
   Size:        163     116
 
AleI CACnn'nnGTG

Cuts at:      0     188     279 
   Size:        188      91
 
ApoI r'AATT_y

Cuts at:      0     256     279 
   Size:        256      23
 
BanI G'GyrC_C

Cuts at:      0      78     279 
   Size:         78     201
 
Bpu10I CC'TnA_GC

Cuts at:      0     272     279 
   Size:        272       7
 
BsrDI GCAATG_nn'

Cuts at:      0     227     279 
   Size:        227      52
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     121     279 
   Size:        121     158
 
BstBI TT'CG_AA

Cuts at:      0      63     279 
   Size:         63     216
 
EarI CTCTTCn'nnn_

Cuts at:      0     139     279 
   Size:        139     140
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     279 
   Size:        163     116
 
EcoRI G'AATT_C

Cuts at:      0     256     279 
   Size:        256      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      41      66      73     279 
   Size:         34       7      25       7     206

  Fragments arranged by size: 

                 206      34      25       7       7
 
HincII GTy'rAC

Cuts at:      0       5     279 
   Size:          5     274
 
MfeI C'AATT_G

Cuts at:      0     116     279 
   Size:        116     163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     279 
   Size:         75     204
 
MslI CAynn'nnrTG

Cuts at:      0     188     190     279 
   Size:        188       2      89

  Fragments arranged by size: 

                 188      89       2
 
NdeI CA'TA_TG

Cuts at:      0      33     126     279 
   Size:         33      93     153

  Fragments arranged by size: 

                 153      93      33
 
NspI r_CATG'y

Cuts at:      0     189     279 
   Size:        189      90
 
SapI GCTCTTCn'nnn_

Cuts at:      0     139     279 
   Size:        139     140
 
StyI C'CwwG_G

Cuts at:      0     201     279 
   Size:        201      78
 

 Enzymes that do cut:

     AcuI     AleI     ApoI     BanI   Bpu10I    BsrDI   BstAPI    BstBI
     EarI  Eco57MI    EcoRI    Hin4I   HincII     MfeI     MmeI     MslI
     NdeI     NspI     SapI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     MscI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI