Restriction map for pF1KE0163
 (Linear) (Six-base) MAPSORT of: KIEE0163.seq  Check: 5194  from: 1  to: 339



KIEE0163.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

Acc65I G'GTAC_C

Cuts at:      0     107     339 
   Size:        107     232
 
AfeI AGC'GCT

Cuts at:      0     265     339 
   Size:        265      74
 
AflIII A'CryG_T

Cuts at:      0     225     339 
   Size:        225     114
 
ApaLI G'TGCA_C

Cuts at:      0     128     339 
   Size:        128     211
 
ApoI r'AATT_y

Cuts at:      0     244     339 
   Size:        244      95
 
BanI G'GyrC_C

Cuts at:      0     107     339 
   Size:        107     232
 
BanII G_rGCy'C

Cuts at:      0     165     339 
   Size:        165     174
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      13      47     339 
   Size:         13      34     292

  Fragments arranged by size: 

                 292      34      13
 
Bme1580I G_kGCm'C

Cuts at:      0     132     339 
   Size:        132     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     339 
   Size:        338       1
 
Bpu10I CC'TnA_GC

Cuts at:      0      61     339 
   Size:         61     278
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     339 
   Size:        283      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     301     339 
   Size:        301      38
 
BsaWI w'CCGG_w

Cuts at:      0     218     339 
   Size:        218     121
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     339 
   Size:        289      50
 
BseYI C'CCAG_C

Cuts at:      0     329     339 
   Size:        329      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     177     339 
   Size:        177     162
 
BsiEI CG_ry'CG

Cuts at:      0      78     339 
   Size:         78     261
 
BsiHKAI G_wGCw'C

Cuts at:      0     132     339 
   Size:        132     207
 
Bsp1286I G_dGCh'C

Cuts at:      0     132     165     339 
   Size:        132      33     174

  Fragments arranged by size: 

                 174     132      33
 
BspEI T'CCGG_A

Cuts at:      0     218     339 
   Size:        218     121
 
BsrFI r'CCGG_y

Cuts at:      0      73     339 
   Size:         73     266
 
BstEII G'GTnAC_C

Cuts at:      0     138     339 
   Size:        138     201
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     316     339 
   Size:        316      23
 
Bsu36I CC'TnA_GG

Cuts at:      0       9     339 
   Size:          9     330
 
BtgI C'CryG_G

Cuts at:      0      95     339 
   Size:         95     244
 
EaeI y'GGCC_r

Cuts at:      0      68      75     339 
   Size:         68       7     264

  Fragments arranged by size: 

                 264      68       7
 
EagI C'GGCC_G

Cuts at:      0      75     339 
   Size:         75     264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     338     339 
   Size:        338       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     238     339 
   Size:        115     123     101

  Fragments arranged by size: 

                 123     115     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     339 
   Size:         14     325
 
HaeII r_GCGC'y

Cuts at:      0     267     339 
   Size:        267      72
 
KpnI G_GTAC'C

Cuts at:      0     111     339 
   Size:        111     228
 
MspA1I CmG'CkG

Cuts at:      0      97     339 
   Size:         97     242
 
NruI TCG'CGA

Cuts at:      0      89     339 
   Size:         89     250
 
NspI r_CATG'y

Cuts at:      0     229     339 
   Size:        229     110
 
PciI A'CATG_T

Cuts at:      0     225     339 
   Size:        225     114
 
PpuMI rG'GwC_Cy

Cuts at:      0      14     339 
   Size:         14     325
 
SacII CC_GC'GG

Cuts at:      0      98     339 
   Size:         98     241
 
SanDI GG'GwC_CC

Cuts at:      0      14     339 
   Size:         14     325
 
SmlI C'TyrA_G

Cuts at:      0     298     339 
   Size:        298      41
 
XmnI GAAnn'nnTTC

Cuts at:      0     121     339 
   Size:        121     218
 

 Enzymes that do cut:

   Acc65I     AfeI   AflIII    ApaLI     ApoI     BanI    BanII     BcgI
 Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BsrFI   BstEII    BstXI
   Bsu36I     BtgI     EaeI     EagI  Eco57MI    EcoNI EcoO109I    HaeII
     KpnI   MspA1I     NruI     NspI     PciI    PpuMI    SacII    SanDI
     SmlI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaXI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI
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