(Linear) (Six-base) MAPSORT of: KIEE0163.seq Check: 5194 from: 1 to: 339 KIEE0163.orf With 182 enzymes: SgfI * April 24, 2009 13:11 .. Acc65I G'GTAC_C Cuts at: 0 107 339 Size: 107 232 AfeI AGC'GCT Cuts at: 0 265 339 Size: 265 74 AflIII A'CryG_T Cuts at: 0 225 339 Size: 225 114 ApaLI G'TGCA_C Cuts at: 0 128 339 Size: 128 211 ApoI r'AATT_y Cuts at: 0 244 339 Size: 244 95 BanI G'GyrC_C Cuts at: 0 107 339 Size: 107 232 BanII G_rGCy'C Cuts at: 0 165 339 Size: 165 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 13 47 339 Size: 13 34 292 Fragments arranged by size: 292 34 13 Bme1580I G_kGCm'C Cuts at: 0 132 339 Size: 132 207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 339 Size: 338 1 Bpu10I CC'TnA_GC Cuts at: 0 61 339 Size: 61 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 339 Size: 283 56 BsaI GGTCTCn'nnnn_ Cuts at: 0 301 339 Size: 301 38 BsaWI w'CCGG_w Cuts at: 0 218 339 Size: 218 121 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 289 339 Size: 289 50 BseYI C'CCAG_C Cuts at: 0 329 339 Size: 329 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 177 339 Size: 177 162 BsiEI CG_ry'CG Cuts at: 0 78 339 Size: 78 261 BsiHKAI G_wGCw'C Cuts at: 0 132 339 Size: 132 207 Bsp1286I G_dGCh'C Cuts at: 0 132 165 339 Size: 132 33 174 Fragments arranged by size: 174 132 33 BspEI T'CCGG_A Cuts at: 0 218 339 Size: 218 121 BsrFI r'CCGG_y Cuts at: 0 73 339 Size: 73 266 BstEII G'GTnAC_C Cuts at: 0 138 339 Size: 138 201 BstXI CCAn_nnnn'nTGG Cuts at: 0 316 339 Size: 316 23 Bsu36I CC'TnA_GG Cuts at: 0 9 339 Size: 9 330 BtgI C'CryG_G Cuts at: 0 95 339 Size: 95 244 EaeI y'GGCC_r Cuts at: 0 68 75 339 Size: 68 7 264 Fragments arranged by size: 264 68 7 EagI C'GGCC_G Cuts at: 0 75 339 Size: 75 264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 339 Size: 338 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 115 238 339 Size: 115 123 101 Fragments arranged by size: 123 115 101 EcoO109I rG'GnC_Cy Cuts at: 0 14 339 Size: 14 325 HaeII r_GCGC'y Cuts at: 0 267 339 Size: 267 72 KpnI G_GTAC'C Cuts at: 0 111 339 Size: 111 228 MspA1I CmG'CkG Cuts at: 0 97 339 Size: 97 242 NruI TCG'CGA Cuts at: 0 89 339 Size: 89 250 NspI r_CATG'y Cuts at: 0 229 339 Size: 229 110 PciI A'CATG_T Cuts at: 0 225 339 Size: 225 114 PpuMI rG'GwC_Cy Cuts at: 0 14 339 Size: 14 325 SacII CC_GC'GG Cuts at: 0 98 339 Size: 98 241 SanDI GG'GwC_CC Cuts at: 0 14 339 Size: 14 325 SmlI C'TyrA_G Cuts at: 0 298 339 Size: 298 41 XmnI GAAnn'nnTTC Cuts at: 0 121 339 Size: 121 218 Enzymes that do cut: Acc65I AfeI AflIII ApaLI ApoI BanI BanII BcgI Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstEII BstXI Bsu36I BtgI EaeI EagI Eco57MI EcoNI EcoO109I HaeII KpnI MspA1I NruI NspI PciI PpuMI SacII SanDI SmlI XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI