(Linear) (Six-base) MAPSORT of: KIEE0163.seq Check: 5194 from: 1 to: 339
KIEE0163.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 107 339
Size: 107 232
AfeI AGC'GCT
Cuts at: 0 265 339
Size: 265 74
AflIII A'CryG_T
Cuts at: 0 225 339
Size: 225 114
ApaLI G'TGCA_C
Cuts at: 0 128 339
Size: 128 211
ApoI r'AATT_y
Cuts at: 0 244 339
Size: 244 95
BanI G'GyrC_C
Cuts at: 0 107 339
Size: 107 232
BanII G_rGCy'C
Cuts at: 0 165 339
Size: 165 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 13 47 339
Size: 13 34 292
Fragments arranged by size:
292 34 13
Bme1580I G_kGCm'C
Cuts at: 0 132 339
Size: 132 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 339
Size: 338 1
Bpu10I CC'TnA_GC
Cuts at: 0 61 339
Size: 61 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 339
Size: 283 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 339
Size: 301 38
BsaWI w'CCGG_w
Cuts at: 0 218 339
Size: 218 121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 289 339
Size: 289 50
BseYI C'CCAG_C
Cuts at: 0 329 339
Size: 329 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 339
Size: 177 162
BsiEI CG_ry'CG
Cuts at: 0 78 339
Size: 78 261
BsiHKAI G_wGCw'C
Cuts at: 0 132 339
Size: 132 207
Bsp1286I G_dGCh'C
Cuts at: 0 132 165 339
Size: 132 33 174
Fragments arranged by size:
174 132 33
BspEI T'CCGG_A
Cuts at: 0 218 339
Size: 218 121
BsrFI r'CCGG_y
Cuts at: 0 73 339
Size: 73 266
BstEII G'GTnAC_C
Cuts at: 0 138 339
Size: 138 201
BstXI CCAn_nnnn'nTGG
Cuts at: 0 316 339
Size: 316 23
Bsu36I CC'TnA_GG
Cuts at: 0 9 339
Size: 9 330
BtgI C'CryG_G
Cuts at: 0 95 339
Size: 95 244
EaeI y'GGCC_r
Cuts at: 0 68 75 339
Size: 68 7 264
Fragments arranged by size:
264 68 7
EagI C'GGCC_G
Cuts at: 0 75 339
Size: 75 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 339
Size: 338 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 238 339
Size: 115 123 101
Fragments arranged by size:
123 115 101
EcoO109I rG'GnC_Cy
Cuts at: 0 14 339
Size: 14 325
HaeII r_GCGC'y
Cuts at: 0 267 339
Size: 267 72
KpnI G_GTAC'C
Cuts at: 0 111 339
Size: 111 228
MspA1I CmG'CkG
Cuts at: 0 97 339
Size: 97 242
NruI TCG'CGA
Cuts at: 0 89 339
Size: 89 250
NspI r_CATG'y
Cuts at: 0 229 339
Size: 229 110
PciI A'CATG_T
Cuts at: 0 225 339
Size: 225 114
PpuMI rG'GwC_Cy
Cuts at: 0 14 339
Size: 14 325
SacII CC_GC'GG
Cuts at: 0 98 339
Size: 98 241
SanDI GG'GwC_CC
Cuts at: 0 14 339
Size: 14 325
SmlI C'TyrA_G
Cuts at: 0 298 339
Size: 298 41
XmnI GAAnn'nnTTC
Cuts at: 0 121 339
Size: 121 218
Enzymes that do cut:
Acc65I AfeI AflIII ApaLI ApoI BanI BanII BcgI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BstEII BstXI
Bsu36I BtgI EaeI EagI Eco57MI EcoNI EcoO109I HaeII
KpnI MspA1I NruI NspI PciI PpuMI SacII SanDI
SmlI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI