(Linear) (Six-base) MAPSORT of: KIEE0166.seq  Check: 7897  from: 1  to: 744



KIEE0166.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AfeI AGC'GCT

Cuts at:      0      75     669     744 
   Size:         75     594      75

  Fragments arranged by size: 

                 594      75      75
 
AhdI GACnn_n'nnGTC

Cuts at:      0     521     744 
   Size:        521     223
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     415     744 
   Size:        415     329
 
BanII G_rGCy'C

Cuts at:      0      95     744 
   Size:         95     649
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     413     470     744 
   Size:        413      57     274

  Fragments arranged by size: 

                 413     274      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     329     551     668     744 
   Size:        320       9     222     117      76

  Fragments arranged by size: 

                 320     222     117      76       9
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     744 
   Size:          5     739
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     288     744 
   Size:         49     239     456

  Fragments arranged by size: 

                 456     239      49
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     132     162     744 
   Size:        132      30     582

  Fragments arranged by size: 

                 582     132      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75     744 
   Size:         75     669
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     744 
   Size:        194     550
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     744 
   Size:         95     649
 
BsrDI GCAATG_nn'

Cuts at:      0     242     744 
   Size:        242     502
 
BstYI r'GATC_y

Cuts at:      0      64     744 
   Size:         64     680
 
Bsu36I CC'TnA_GG

Cuts at:      0      58     744 
   Size:         58     686
 
BtgI C'CryG_G

Cuts at:      0      87     744 
   Size:         87     657
 
BtsI GCAGTG_nn'

Cuts at:      0     399     639     744 
   Size:        399     240     105

  Fragments arranged by size: 

                 399     240     105
 
EarI CTCTTCn'nnn_

Cuts at:      0     544     714     744 
   Size:        544     170      30

  Fragments arranged by size: 

                 544     170      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     329     551     668     744 
   Size:        320       9     222     117      76

  Fragments arranged by size: 

                 320     222     117      76       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     106     744 
   Size:        106     638
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     555     744 
   Size:        555     189
 
HaeII r_GCGC'y

Cuts at:      0      77     671     744 
   Size:         77     594      73

  Fragments arranged by size: 

                 594      77      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     714     718     744 
   Size:        507      32     175       4      26

  Fragments arranged by size: 

                 507     175      32      26       4
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     744 
   Size:        141     603
 
MslI CAynn'nnrTG

Cuts at:      0     721     744 
   Size:        721      23
 
MspA1I CmG'CkG

Cuts at:      0     654     744 
   Size:        654      90
 
NcoI C'CATG_G

Cuts at:      0      87     744 
   Size:         87     657
 
NspI r_CATG'y

Cuts at:      0     695     744 
   Size:        695      49
 
SapI GCTCTTCn'nnn_

Cuts at:      0     714     744 
   Size:        714      30
 
SmlI C'TyrA_G

Cuts at:      0      28     267     744 
   Size:         28     239     477

  Fragments arranged by size: 

                 477     239      28
 
SphI G_CATG'C

Cuts at:      0     695     744 
   Size:        695      49
 
StuI AGG'CCT

Cuts at:      0     185     744 
   Size:        185     559
 
StyI C'CwwG_G

Cuts at:      0      87     543     744 
   Size:         87     456     201

  Fragments arranged by size: 

                 456     201      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     372     744 
   Size:        372     372
 
TatI w'GTAC_w

Cuts at:      0     148     744 
   Size:        148     596
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130     744 
   Size:         98      32     614

  Fragments arranged by size: 

                 614      98      32
 

 Enzymes that do cut:

     AfeI     AhdI     AlfI    BanII     BmrI     BpmI   Bpu10I    BpuEI
    BsaXI    BseRI     BsgI Bsp1286I    BsrDI    BstYI   Bsu36I     BtgI
     BtsI     EarI  Eco57MI    EcoNI     FalI    HaeII    Hin4I     MmeI
     MslI   MspA1I     NcoI     NspI     SapI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI