(Linear) (Six-base) MAPSORT of: KIEE0171.seq  Check: 4874  from: 1  to: 666



KIEE0171.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     283     556     666 
   Size:        235      48     273     110

  Fragments arranged by size: 

                 273     235     110      48
 
BamHI G'GATC_C

Cuts at:      0     501     666 
   Size:        501     165
 
BanI G'GyrC_C

Cuts at:      0      22     106     666 
   Size:         22      84     560

  Fragments arranged by size: 

                 560      84      22
 
BanII G_rGCy'C

Cuts at:      0      64     666 
   Size:         64     602
 
BbeI G_GCGC'C

Cuts at:      0     110     666 
   Size:        110     556
 
BbvCI CC'TCA_GC

Cuts at:      0     313     666 
   Size:        313     353
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     636     666 
   Size:        636      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     666 
   Size:         48     618
 
Bpu10I CC'TnA_GC

Cuts at:      0     313     432     666 
   Size:        313     119     234

  Fragments arranged by size: 

                 313     234     119
 
BsaAI yAC'GTr

Cuts at:      0     174     666 
   Size:        174     492
 
BsaHI Gr'CG_yC

Cuts at:      0     107     666 
   Size:        107     559
 
BsaWI w'CCGG_w

Cuts at:      0      42     666 
   Size:         42     624
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211     666 
   Size:        211     455
 
BseYI C'CCAG_C

Cuts at:      0     537     666 
   Size:        537     129
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     242     666 
   Size:        130     112     424

  Fragments arranged by size: 

                 424     130     112
 
BsiHKAI G_wGCw'C

Cuts at:      0     560     666 
   Size:        560     106
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     560     666 
   Size:         64     496     106

  Fragments arranged by size: 

                 496     106      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      98     666 
   Size:         98     568
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     138     666 
   Size:        138     528
 
BstYI r'GATC_y

Cuts at:      0     501     666 
   Size:        501     165
 
BtsI GCAGTG_nn'

Cuts at:      0      20     505     532     666 
   Size:         20     485      27     134

  Fragments arranged by size: 

                 485     134      27      20
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     258     293     666 
   Size:        258      35     373

  Fragments arranged by size: 

                 373     258      35
 
EaeI y'GGCC_r

Cuts at:      0     218     666 
   Size:        218     448
 
EarI CTCTTCn'nnn_

Cuts at:      0     254     666 
   Size:        254     412
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     235     283     556     666 
   Size:         48     187      48     273     110

  Fragments arranged by size: 

                 273     187     110      48      48
 
HaeII r_GCGC'y

Cuts at:      0     110     666 
   Size:        110     556
 
KasI G'GCGC_C

Cuts at:      0     106     666 
   Size:        106     560
 
MscI TGG'CCA

Cuts at:      0     220     666 
   Size:        220     446
 
MspA1I CmG'CkG

Cuts at:      0      36     144     666 
   Size:         36     108     522

  Fragments arranged by size: 

                 522     108      36
 
NarI GG'CG_CC

Cuts at:      0     107     666 
   Size:        107     559
 
PvuII CAG'CTG

Cuts at:      0      36     144     666 
   Size:         36     108     522

  Fragments arranged by size: 

                 522     108      36
 
SapI GCTCTTCn'nnn_

Cuts at:      0     254     666 
   Size:        254     412
 
SfoI GGC'GCC

Cuts at:      0     108     666 
   Size:        108     558
 
StuI AGG'CCT

Cuts at:      0     524     591     666 
   Size:        524      67      75

  Fragments arranged by size: 

                 524      75      67
 
StyI C'CwwG_G

Cuts at:      0     221     426     666 
   Size:        221     205     240

  Fragments arranged by size: 

                 240     221     205
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     666 
   Size:         33     633
 

 Enzymes that do cut:

     AcuI    BamHI     BanI    BanII     BbeI    BbvCI     BmrI     BpmI
   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI   BstAPI    BstYI     BtsI    CspCI     EaeI     EarI
  Eco57MI    HaeII     KasI     MscI   MspA1I     NarI    PvuII     SapI
     SfoI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI