Restriction map for
pF1KE0173
(Linear) (Six-base) MAPSORT of: KIEE0173.seq Check: 5371 from: 1 to: 894
KIEE0173.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 426 894
Size: 426 468
AatII G_ACGT'C
Cuts at: 0 47 894
Size: 47 847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 894
Size: 780 114
AflIII A'CryG_T
Cuts at: 0 125 894
Size: 125 769
AlwNI CAG_nnn'CTG
Cuts at: 0 18 894
Size: 18 876
ApaI G_GGCC'C
Cuts at: 0 703 894
Size: 703 191
BamHI G'GATC_C
Cuts at: 0 404 894
Size: 404 490
BanI G'GyrC_C
Cuts at: 0 685 842 894
Size: 685 157 52
Fragments arranged by size:
685 157 52
BanII G_rGCy'C
Cuts at: 0 165 184 536 703 894
Size: 165 19 352 167 191
Fragments arranged by size:
352 191 167 165 19
BbeI G_GCGC'C
Cuts at: 0 689 894
Size: 689 205
BbvCI CC'TCA_GC
Cuts at: 0 677 894
Size: 677 217
BciVI GTATCCnnnnn_n'
Cuts at: 0 747 894
Size: 747 147
BglI GCCn_nnn'nGGC
Cuts at: 0 707 875 894
Size: 707 168 19
Fragments arranged by size:
707 168 19
Bme1580I G_kGCm'C
Cuts at: 0 703 894
Size: 703 191
BmgBI CAC'GTC
Cuts at: 0 561 894
Size: 561 333
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 769 894
Size: 387 382 125
Fragments arranged by size:
387 382 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 550 894
Size: 550 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 788 894
Size: 467 321 106
Fragments arranged by size:
467 321 106
Bpu10I CC'TnA_GC
Cuts at: 0 677 894
Size: 677 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 894
Size: 642 252
BsaHI Gr'CG_yC
Cuts at: 0 44 686 692 894
Size: 44 642 6 202
Fragments arranged by size:
642 202 44 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 548 578 894
Size: 76 30 442 30 316
Fragments arranged by size:
442 316 76 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 544 694 828 894
Size: 544 150 134 66
Fragments arranged by size:
544 150 134 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 841 894
Size: 123 718 53
Fragments arranged by size:
718 123 53
BsiHKAI G_wGCw'C
Cuts at: 0 536 894
Size: 536 358
BsmBI CGTCTCn'nnnn_
Cuts at: 0 551 894
Size: 551 343
Bsp1286I G_dGCh'C
Cuts at: 0 165 184 536 703 894
Size: 165 19 352 167 191
Fragments arranged by size:
352 191 167 165 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 426 894
Size: 426 468
BsrFI r'CCGG_y
Cuts at: 0 853 894
Size: 853 41
BssSI C'ACGA_G
Cuts at: 0 524 894
Size: 524 370
BstXI CCAn_nnnn'nTGG
Cuts at: 0 547 594 894
Size: 547 47 300
Fragments arranged by size:
547 300 47
BstYI r'GATC_y
Cuts at: 0 14 404 494 894
Size: 14 390 90 400
Fragments arranged by size:
400 390 90 14
Bsu36I CC'TnA_GG
Cuts at: 0 71 894
Size: 71 823
BtgI C'CryG_G
Cuts at: 0 290 506 894
Size: 290 216 388
Fragments arranged by size:
388 290 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 270 894
Size: 270 624
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 846 881 894
Size: 846 35 13
Fragments arranged by size:
846 35 13
EaeI y'GGCC_r
Cuts at: 0 195 584 851 894
Size: 195 389 267 43
Fragments arranged by size:
389 267 195 43
EarI CTCTTCn'nnn_
Cuts at: 0 541 894
Size: 541 353
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 217 894
Size: 217 677
EcoICRI GAG'CTC
Cuts at: 0 534 894
Size: 534 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 780 788 894
Size: 467 313 8 106
Fragments arranged by size:
467 313 106 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 805 894
Size: 805 89
EcoO109I rG'GnC_Cy
Cuts at: 0 700 894
Size: 700 194
HaeII r_GCGC'y
Cuts at: 0 689 894
Size: 689 205
KasI G'GCGC_C
Cuts at: 0 685 894
Size: 685 209
MluI A'CGCG_T
Cuts at: 0 125 894
Size: 125 769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 894
Size: 155 739
MscI TGG'CCA
Cuts at: 0 197 586 894
Size: 197 389 308
Fragments arranged by size:
389 308 197
MslI CAynn'nnrTG
Cuts at: 0 725 894
Size: 725 169
MspA1I CmG'CkG
Cuts at: 0 453 543 894
Size: 453 90 351
Fragments arranged by size:
453 351 90
NaeI GCC'GGC
Cuts at: 0 855 894
Size: 855 39
NarI GG'CG_CC
Cuts at: 0 686 894
Size: 686 208
NgoMIV G'CCGG_C
Cuts at: 0 853 894
Size: 853 41
PasI CC'CwG_GG
Cuts at: 0 696 807 894
Size: 696 111 87
Fragments arranged by size:
696 111 87
PfoI T'CCnGG_A
Cuts at: 0 215 894
Size: 215 679
PspOMI G'GGCC_C
Cuts at: 0 699 894
Size: 699 195
PstI C_TGCA'G
Cuts at: 0 452 875 894
Size: 452 423 19
Fragments arranged by size:
452 423 19
PvuII CAG'CTG
Cuts at: 0 543 894
Size: 543 351
SacI G_AGCT'C
Cuts at: 0 536 894
Size: 536 358
SapI GCTCTTCn'nnn_
Cuts at: 0 541 894
Size: 541 353
SbfI CC_TGCA'GG
Cuts at: 0 875 894
Size: 875 19
SfcI C'TryA_G
Cuts at: 0 365 448 871 894
Size: 365 83 423 23
Fragments arranged by size:
423 365 83 23
SfoI GGC'GCC
Cuts at: 0 687 894
Size: 687 207
SmlI C'TyrA_G
Cuts at: 0 621 894
Size: 621 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 76 108 548 580 894
Size: 76 32 440 32 314
Fragments arranged by size:
440 314 76 32 32
ZraI GAC'GTC
Cuts at: 0 45 894
Size: 45 849
Enzymes that do cut:
AarI AatII AcuI AflIII AlwNI ApaI BamHI BanI
BanII BbeI BbvCI BciVI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstXI BstYI
Bsu36I BtgI BtgZI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PasI PfoI PspOMI
PstI PvuII SacI SapI SbfI SfcI SfoI SmlI
TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbsI BcgI BclI BfrBI BglII BlpI
BmtI BsaI BsaAI BsaBI BsaWI BseYI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtsI ClaI DraI DraIII DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI