Restriction map for
pF1KE0177
(Linear) (Six-base) MAPSORT of: KIEE0177.seq Check: 1136 from: 1 to: 732
KIEE0177.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 732
Size: 321 411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 619 732
Size: 619 113
AlwNI CAG_nnn'CTG
Cuts at: 0 197 433 732
Size: 197 236 299
Fragments arranged by size:
299 236 197
BanI G'GyrC_C
Cuts at: 0 295 348 732
Size: 295 53 384
Fragments arranged by size:
384 295 53
BbvCI CC'TCA_GC
Cuts at: 0 274 732
Size: 274 458
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 267 301 732
Size: 267 34 431
Fragments arranged by size:
431 267 34
BclI T'GATC_A
Cuts at: 0 113 732
Size: 113 619
BfrBI ATG'CAT
Cuts at: 0 651 732
Size: 651 81
Bme1580I G_kGCm'C
Cuts at: 0 177 300 353 732
Size: 177 123 53 379
Fragments arranged by size:
379 177 123 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 732
Size: 638 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 241 732
Size: 117 124 491
Fragments arranged by size:
491 124 117
Bpu10I CC'TnA_GC
Cuts at: 0 274 732
Size: 274 458
BseYI C'CCAG_C
Cuts at: 0 406 544 732
Size: 406 138 188
Fragments arranged by size:
406 188 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 732
Size: 407 325
Bsp1286I G_dGCh'C
Cuts at: 0 177 300 353 732
Size: 177 123 53 379
Fragments arranged by size:
379 177 123 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 379 418 732
Size: 254 125 39 314
Fragments arranged by size:
314 254 125 39
BsrGI T'GTAC_A
Cuts at: 0 589 732
Size: 589 143
BstAPI GCAn_nnn'nTGC
Cuts at: 0 197 732
Size: 197 535
BstXI CCAn_nnnn'nTGG
Cuts at: 0 375 455 732
Size: 375 80 277
Fragments arranged by size:
375 277 80
BtgI C'CryG_G
Cuts at: 0 290 732
Size: 290 442
BtsI GCAGTG_nn'
Cuts at: 0 198 329 527 732
Size: 198 131 198 205
Fragments arranged by size:
205 198 198 131
DraIII CAC_nnn'GTG
Cuts at: 0 402 732
Size: 402 330
DrdI GACnn_nn'nnGTC
Cuts at: 0 250 732
Size: 250 482
EaeI y'GGCC_r
Cuts at: 0 365 732
Size: 365 367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 241 321 732
Size: 117 124 80 411
Fragments arranged by size:
411 124 117 80
EcoO109I rG'GnC_Cy
Cuts at: 0 341 549 732
Size: 341 208 183
Fragments arranged by size:
341 208 183
HincII GTy'rAC
Cuts at: 0 234 732
Size: 234 498
MscI TGG'CCA
Cuts at: 0 367 732
Size: 367 365
MspA1I CmG'CkG
Cuts at: 0 240 732
Size: 240 492
NcoI C'CATG_G
Cuts at: 0 290 732
Size: 290 442
NsiI A_TGCA'T
Cuts at: 0 653 732
Size: 653 79
NspI r_CATG'y
Cuts at: 0 282 596 732
Size: 282 314 136
Fragments arranged by size:
314 282 136
PflMI CCAn_nnn'nTGG
Cuts at: 0 402 732
Size: 402 330
PpuMI rG'GwC_Cy
Cuts at: 0 341 732
Size: 341 391
PsiI TTA'TAA
Cuts at: 0 470 732
Size: 470 262
PvuII CAG'CTG
Cuts at: 0 240 732
Size: 240 492
SexAI A'CCwGG_T
Cuts at: 0 398 732
Size: 398 334
SphI G_CATG'C
Cuts at: 0 282 732
Size: 282 450
StuI AGG'CCT
Cuts at: 0 68 158 732
Size: 68 90 574
Fragments arranged by size:
574 90 68
StyI C'CwwG_G
Cuts at: 0 290 732
Size: 290 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 283 657 732
Size: 153 130 374 75
Fragments arranged by size:
374 153 130 75
TatI w'GTAC_w
Cuts at: 0 589 629 732
Size: 589 40 103
Fragments arranged by size:
589 103 40
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 360 403 732
Size: 360 43 329
Fragments arranged by size:
360 329 43
Enzymes that do cut:
AcuI AlfI AlwNI BanI BbvCI BcgI BclI BfrBI
Bme1580I BmrI BpmI Bpu10I BseYI BsgI Bsp1286I BspMI
BsrGI BstAPI BstXI BtgI BtsI DraIII DrdI EaeI
Eco57MI EcoO109I HincII MscI MspA1I NcoI NsiI NspI
PflMI PpuMI PsiI PvuII SexAI SphI StuI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbsI BciVI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI