Restriction map for
pF1KE0179
(Linear) (Six-base) MAPSORT of: KIEE0179.seq Check: 670 from: 1 to: 1026
KIEE0179.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 0 596 1026
Size: 596 430
AlwNI CAG_nnn'CTG
Cuts at: 0 867 1026
Size: 867 159
ApaLI G'TGCA_C
Cuts at: 0 155 1026
Size: 155 871
ApoI r'AATT_y
Cuts at: 0 956 1026
Size: 956 70
AvaI C'yCGr_G
Cuts at: 0 24 294 1026
Size: 24 270 732
Fragments arranged by size:
732 270 24
AvrII C'CTAG_G
Cuts at: 0 993 1026
Size: 993 33
BanII G_rGCy'C
Cuts at: 0 88 110 266 519 724 1026
Size: 88 22 156 253 205 302
Fragments arranged by size:
302 253 205 156 88 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 1026
Size: 658 368
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 285 319 387 421 1026
Size: 285 34 68 34 605
Fragments arranged by size:
605 285 68 34 34
BclI T'GATC_A
Cuts at: 0 633 1026
Size: 633 393
BglI GCCn_nnn'nGGC
Cuts at: 0 92 306 1026
Size: 92 214 720
Fragments arranged by size:
720 214 92
BlpI GC'TnA_GC
Cuts at: 0 839 1026
Size: 839 187
Bme1580I G_kGCm'C
Cuts at: 0 72 159 255 1026
Size: 72 87 96 771
Fragments arranged by size:
771 96 87 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 399 722 1026
Size: 399 323 304
Fragments arranged by size:
399 323 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 98 628 1026
Size: 84 14 530 398
Fragments arranged by size:
530 398 84 14
BsaWI w'CCGG_w
Cuts at: 0 137 1026
Size: 137 889
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 933 963 1026
Size: 933 30 63
Fragments arranged by size:
933 63 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 752 1026
Size: 42 710 274
Fragments arranged by size:
710 274 42
BseYI C'CCAG_C
Cuts at: 0 95 540 911 1026
Size: 95 445 371 115
Fragments arranged by size:
445 371 115 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1026
Size: 640 386
BsiHKAI G_wGCw'C
Cuts at: 0 110 159 1026
Size: 110 49 867
Fragments arranged by size:
867 110 49
Bsp1286I G_dGCh'C
Cuts at: 0 72 88 110 159 255 266 519 724
Size: 72 16 22 49 96 11 253 205
Cuts at: 724 1026
Size: 302
Fragments arranged by size:
302 253 205 96 72 49 22 16
11
BspEI T'CCGG_A
Cuts at: 0 137 1026
Size: 137 889
BsrDI GCAATG_nn'
Cuts at: 0 40 932 1026
Size: 40 892 94
Fragments arranged by size:
892 94 40
BssSI C'ACGA_G
Cuts at: 0 406 1026
Size: 406 620
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1004 1026
Size: 1004 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 394 1026
Size: 394 632
Bsu36I CC'TnA_GG
Cuts at: 0 493 833 920 1026
Size: 493 340 87 106
Fragments arranged by size:
493 340 106 87
BtgI C'CryG_G
Cuts at: 0 419 1026
Size: 419 607
BtsI GCAGTG_nn'
Cuts at: 0 878 1026
Size: 878 148
DraIII CAC_nnn'GTG
Cuts at: 0 534 1004 1026
Size: 534 470 22
Fragments arranged by size:
534 470 22
EaeI y'GGCC_r
Cuts at: 0 117 416 1026
Size: 117 299 610
Fragments arranged by size:
610 299 117
EarI CTCTTCn'nnn_
Cuts at: 0 182 1026
Size: 182 844
EcoICRI GAG'CTC
Cuts at: 0 108 1026
Size: 108 918
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 98 628 1026
Size: 84 14 530 398
Fragments arranged by size:
530 398 84 14
EcoO109I rG'GnC_Cy
Cuts at: 0 123 216 739 1026
Size: 123 93 523 287
Fragments arranged by size:
523 287 123 93
EcoRI G'AATT_C
Cuts at: 0 956 1026
Size: 956 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 269 301 423 455 1026
Size: 269 32 122 32 571
Fragments arranged by size:
571 269 122 32 32
MscI TGG'CCA
Cuts at: 0 119 1026
Size: 119 907
MspA1I CmG'CkG
Cuts at: 0 421 505 733 864 1026
Size: 421 84 228 131 162
Fragments arranged by size:
421 228 162 131 84
NspI r_CATG'y
Cuts at: 0 402 1026
Size: 402 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 867 1026
Size: 867 159
PpuMI rG'GwC_Cy
Cuts at: 0 123 216 1026
Size: 123 93 810
Fragments arranged by size:
810 123 93
PspXI vC'TCGA_Gb
Cuts at: 0 294 1026
Size: 294 732
PvuII CAG'CTG
Cuts at: 0 505 864 1026
Size: 505 359 162
Fragments arranged by size:
505 359 162
SacI G_AGCT'C
Cuts at: 0 110 1026
Size: 110 916
SacII CC_GC'GG
Cuts at: 0 422 1026
Size: 422 604
SapI GCTCTTCn'nnn_
Cuts at: 0 182 1026
Size: 182 844
SmaI CCC'GGG
Cuts at: 0 26 1026
Size: 26 1000
SmlI C'TyrA_G
Cuts at: 0 294 1026
Size: 294 732
SphI G_CATG'C
Cuts at: 0 402 1026
Size: 402 624
StuI AGG'CCT
Cuts at: 0 300 1026
Size: 300 726
StyI C'CwwG_G
Cuts at: 0 377 993 1026
Size: 377 616 33
Fragments arranged by size:
616 377 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 1026
Size: 174 852
TatI w'GTAC_w
Cuts at: 0 230 1026
Size: 230 796
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 933 965 1026
Size: 933 32 61
Fragments arranged by size:
933 61 32
XhoI C'TCGA_G
Cuts at: 0 294 1026
Size: 294 732
XmaI C'CCGG_G
Cuts at: 0 24 1026
Size: 24 1002
Enzymes that do cut:
AccI AlwNI ApaLI ApoI AvaI AvrII BanII BbsI
BcgI BclI BglI BlpI Bme1580I BmrI BpmI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrDI
BssSI BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I MscI MspA1I
NspI PflMI PpuMI PspXI PvuII SacI SacII SapI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbvCI BciVI BfrBI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstBI BstEII BstYI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI
XmnI ZraI