Restriction map for
pF1KE0181
(Linear) (Six-base) MAPSORT of: KIEE0181.seq Check: 4525 from: 1 to: 807
KIEE0181.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 0 680 807
Size: 680 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 807
Size: 428 379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 332 505 807
Size: 332 173 302
Fragments arranged by size:
332 302 173
AlwNI CAG_nnn'CTG
Cuts at: 0 72 807
Size: 72 735
ApaI G_GGCC'C
Cuts at: 0 769 807
Size: 769 38
AscI GG'CGCG_CC
Cuts at: 0 85 807
Size: 85 722
BanII G_rGCy'C
Cuts at: 0 290 454 769 807
Size: 290 164 315 38
Fragments arranged by size:
315 290 164 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 201 807
Size: 201 606
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 246 280 807
Size: 246 34 527
Fragments arranged by size:
527 246 34
BclI T'GATC_A
Cuts at: 0 98 807
Size: 98 709
BfrBI ATG'CAT
Cuts at: 0 693 807
Size: 693 114
Bme1580I G_kGCm'C
Cuts at: 0 25 565 769 807
Size: 25 540 204 38
Fragments arranged by size:
540 204 38 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 807
Size: 133 674
BsaAI yAC'GTr
Cuts at: 0 81 807
Size: 81 726
BsaHI Gr'CG_yC
Cuts at: 0 431 527 807
Size: 431 96 280
Fragments arranged by size:
431 280 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 470 500 807
Size: 470 30 307
Fragments arranged by size:
470 307 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 807
Size: 386 421
BseYI C'CCAG_C
Cuts at: 0 222 807
Size: 222 585
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 807
Size: 289 518
BsiEI CG_ry'CG
Cuts at: 0 29 807
Size: 29 778
BsiHKAI G_wGCw'C
Cuts at: 0 454 807
Size: 454 353
Bsp1286I G_dGCh'C
Cuts at: 0 25 290 454 565 769 807
Size: 25 265 164 111 204 38
Fragments arranged by size:
265 204 164 111 38 25
BspHI T'CATG_A
Cuts at: 0 506 807
Size: 506 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 197 807
Size: 80 117 610
Fragments arranged by size:
610 117 80
BssHII G'CGCG_C
Cuts at: 0 85 807
Size: 85 722
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 807
Size: 72 735
BtgI C'CryG_G
Cuts at: 0 782 807
Size: 782 25
DraI TTT'AAA
Cuts at: 0 597 807
Size: 597 210
EaeI y'GGCC_r
Cuts at: 0 26 584 807
Size: 26 558 223
Fragments arranged by size:
558 223 26
EagI C'GGCC_G
Cuts at: 0 26 807
Size: 26 781
EarI CTCTTCn'nnn_
Cuts at: 0 409 807
Size: 409 398
EcoICRI GAG'CTC
Cuts at: 0 452 807
Size: 452 355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 428 807
Size: 133 295 379
Fragments arranged by size:
379 295 133
EcoO109I rG'GnC_Cy
Cuts at: 0 766 807
Size: 766 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 807
Size: 470 32 305
Fragments arranged by size:
470 305 32
MscI TGG'CCA
Cuts at: 0 586 807
Size: 586 221
MslI CAynn'nnrTG
Cuts at: 0 127 730 807
Size: 127 603 77
Fragments arranged by size:
603 127 77
MspA1I CmG'CkG
Cuts at: 0 231 807
Size: 231 576
NcoI C'CATG_G
Cuts at: 0 782 807
Size: 782 25
NsiI A_TGCA'T
Cuts at: 0 695 807
Size: 695 112
NspI r_CATG'y
Cuts at: 0 423 471 807
Size: 423 48 336
Fragments arranged by size:
423 336 48
PasI CC'CwG_GG
Cuts at: 0 366 762 769 807
Size: 366 396 7 38
Fragments arranged by size:
396 366 38 7
PfoI T'CCnGG_A
Cuts at: 0 110 807
Size: 110 697
PmlI CAC'GTG
Cuts at: 0 81 807
Size: 81 726
PspOMI G'GGCC_C
Cuts at: 0 765 807
Size: 765 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 169 201 807
Size: 169 32 606
Fragments arranged by size:
606 169 32
PstI C_TGCA'G
Cuts at: 0 311 807
Size: 311 496
PvuII CAG'CTG
Cuts at: 0 231 807
Size: 231 576
SacI G_AGCT'C
Cuts at: 0 454 807
Size: 454 353
SapI GCTCTTCn'nnn_
Cuts at: 0 409 807
Size: 409 398
SfcI C'TryA_G
Cuts at: 0 307 321 807
Size: 307 14 486
Fragments arranged by size:
486 307 14
StuI AGG'CCT
Cuts at: 0 377 807
Size: 377 430
StyI C'CwwG_G
Cuts at: 0 782 807
Size: 782 25
TatI w'GTAC_w
Cuts at: 0 464 807
Size: 464 343
Tth111I GACn'n_nGTC
Cuts at: 0 726 807
Size: 726 81
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI AscI BanII BbsI
BcgI BclI BfrBI Bme1580I BpmI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BssHII BstAPI BtgI DraI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I Hin4I MscI MslI MspA1I NcoI NsiI
NspI PasI PfoI PmlI PspOMI PsrI PstI PvuII
SacI SapI SfcI StuI StyI TatI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII TstI XbaI
XcmI XhoI XmaI XmnI ZraI