Restriction map for
pF1KE0184
(Linear) (Six-base) MAPSORT of: KIEE0184.seq Check: 5553 from: 1 to: 858
KIEE0184.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 709 858
Size: 618 91 149
Fragments arranged by size:
618 149 91
AflIII A'CryG_T
Cuts at: 0 344 858
Size: 344 514
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 858
Size: 94 764
AlwNI CAG_nnn'CTG
Cuts at: 0 504 521 858
Size: 504 17 337
Fragments arranged by size:
504 337 17
ApaLI G'TGCA_C
Cuts at: 0 697 858
Size: 697 161
ApoI r'AATT_y
Cuts at: 0 272 445 454 858
Size: 272 173 9 404
Fragments arranged by size:
404 272 173 9
AvaI C'yCGr_G
Cuts at: 0 119 858
Size: 119 739
BanII G_rGCy'C
Cuts at: 0 621 858
Size: 621 237
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 743 847 858
Size: 131 612 104 11
Fragments arranged by size:
612 131 104 11
BglII A'GATC_T
Cuts at: 0 115 858
Size: 115 743
Bme1580I G_kGCm'C
Cuts at: 0 701 858
Size: 701 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 624 858
Size: 624 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 471 838 858
Size: 402 69 367 20
Fragments arranged by size:
402 367 69 20
BsaAI yAC'GTr
Cuts at: 0 347 858
Size: 347 511
BsaWI w'CCGG_w
Cuts at: 0 89 152 246 544 858
Size: 89 63 94 298 314
Fragments arranged by size:
314 298 94 89 63
BseYI C'CCAG_C
Cuts at: 0 770 858
Size: 770 88
BsiHKAI G_wGCw'C
Cuts at: 0 701 858
Size: 701 157
BsmI GAATG_Cn'
Cuts at: 0 256 753 858
Size: 256 497 105
Fragments arranged by size:
497 256 105
Bsp1286I G_dGCh'C
Cuts at: 0 621 701 858
Size: 621 80 157
Fragments arranged by size:
621 157 80
BspEI T'CCGG_A
Cuts at: 0 89 152 544 858
Size: 89 63 392 314
Fragments arranged by size:
392 314 89 63
BspHI T'CATG_A
Cuts at: 0 419 827 858
Size: 419 408 31
Fragments arranged by size:
419 408 31
BsrBI CCG'CTC
Cuts at: 0 31 858
Size: 31 827
BsrFI r'CCGG_y
Cuts at: 0 40 858
Size: 40 818
BsrGI T'GTAC_A
Cuts at: 0 689 858
Size: 689 169
BssHII G'CGCG_C
Cuts at: 0 18 858
Size: 18 840
BssSI C'ACGA_G
Cuts at: 0 620 858
Size: 620 238
BstYI r'GATC_y
Cuts at: 0 115 541 720 858
Size: 115 426 179 138
Fragments arranged by size:
426 179 138 115
BtgI C'CryG_G
Cuts at: 0 24 858
Size: 24 834
DrdI GACnn_nn'nnGTC
Cuts at: 0 370 858
Size: 370 488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 402 471 618 709 838 858
Size: 402 69 147 91 129 20
Fragments arranged by size:
402 147 129 91 69 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 858
Size: 166 32 660
Fragments arranged by size:
660 166 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 606 639 858
Size: 488 118 33 219
Fragments arranged by size:
488 219 118 33
MslI CAynn'nnrTG
Cuts at: 0 764 858
Size: 764 94
MspA1I CmG'CkG
Cuts at: 0 26 57 131 687 858
Size: 26 31 74 556 171
Fragments arranged by size:
556 171 74 31 26
NaeI GCC'GGC
Cuts at: 0 42 858
Size: 42 816
NgoMIV G'CCGG_C
Cuts at: 0 40 858
Size: 40 818
NspI r_CATG'y
Cuts at: 0 480 858
Size: 480 378
PfoI T'CCnGG_A
Cuts at: 0 487 858
Size: 487 371
PmlI CAC'GTG
Cuts at: 0 347 858
Size: 347 511
PstI C_TGCA'G
Cuts at: 0 56 73 858
Size: 56 17 785
Fragments arranged by size:
785 56 17
PvuII CAG'CTG
Cuts at: 0 131 687 858
Size: 131 556 171
Fragments arranged by size:
556 171 131
SacII CC_GC'GG
Cuts at: 0 27 858
Size: 27 831
SfcI C'TryA_G
Cuts at: 0 52 69 858
Size: 52 17 789
Fragments arranged by size:
789 52 17
SmlI C'TyrA_G
Cuts at: 0 119 858
Size: 119 739
TatI w'GTAC_w
Cuts at: 0 322 689 858
Size: 322 367 169
Fragments arranged by size:
367 322 169
XhoI C'TCGA_G
Cuts at: 0 119 858
Size: 119 739
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI BanII
BbsI BglII Bme1580I BmrI BpmI BsaAI BsaWI BseYI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstYI BtgI DrdI Eco57MI Hin4I MmeI
MslI MspA1I NaeI NgoMIV NspI PfoI PmlI PstI
PvuII SacII SfcI SmlI TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiWI
BsmBI BspMI BsrDI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XmaI XmnI ZraI