(Linear) (Six-base) MAPSORT of: KIEE0186.seq  Check: 8000  from: 1  to: 660



KIEE0186.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AclI AA'CG_TT

Cuts at:      0      39     660 
   Size:         39     621
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     660 
   Size:        394     266
 
AflIII A'CryG_T

Cuts at:      0     125     199     660 
   Size:        125      74     461

  Fragments arranged by size: 

                 461     125      74
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     638     660 
   Size:        638      22
 
ApoI r'AATT_y

Cuts at:      0      52     508     531     660 
   Size:         52     456      23     129

  Fragments arranged by size: 

                 456     129      52      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     196     660 
   Size:        196     464
 
BlpI GC'TnA_GC

Cuts at:      0     577     660 
   Size:        577      83
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      90     354     660 
   Size:         90     264     306

  Fragments arranged by size: 

                 306     264      90
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     229     660 
   Size:        229     431
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     660 
   Size:         68     592
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     641     660 
   Size:        641      19
 
BspHI T'CATG_A

Cuts at:      0     396     512     660 
   Size:        396     116     148

  Fragments arranged by size: 

                 396     148     116
 
BtgI C'CryG_G

Cuts at:      0     189     209     430     660 
   Size:        189      20     221     230

  Fragments arranged by size: 

                 230     221     189      20
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     200     660 
   Size:        200     460
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     660 
   Size:        394     266
 
EcoRI G'AATT_C

Cuts at:      0      52     508     531     660 
   Size:         52     456      23     129

  Fragments arranged by size: 

                 456     129      52      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     660 
   Size:        156      32     472

  Fragments arranged by size: 

                 472     156      32
 
HincII GTy'rAC

Cuts at:      0     558     660 
   Size:        558     102
 
HindIII A'AGCT_T

Cuts at:      0     345     660 
   Size:        345     315
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     660 
   Size:         60     600
 
NcoI C'CATG_G

Cuts at:      0     189     209     430     660 
   Size:        189      20     221     230

  Fragments arranged by size: 

                 230     221     189      20
 
NdeI CA'TA_TG

Cuts at:      0     382     660 
   Size:        382     278
 
NspI r_CATG'y

Cuts at:      0     129     203     660 
   Size:        129      74     457

  Fragments arranged by size: 

                 457     129      74
 
PciI A'CATG_T

Cuts at:      0     125     199     660 
   Size:        125      74     461

  Fragments arranged by size: 

                 461     125      74
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     466     598     660 
   Size:        466     132      62

  Fragments arranged by size: 

                 466     132      62
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     446     478     660 
   Size:        446      32     182

  Fragments arranged by size: 

                 446     182      32
 
PstI C_TGCA'G

Cuts at:      0     264     660 
   Size:        264     396
 
SfcI C'TryA_G

Cuts at:      0     260     309     421     660 
   Size:        260      49     112     239

  Fragments arranged by size: 

                 260     239     112      49
 
StyI C'CwwG_G

Cuts at:      0     189     209     233     430     660 
   Size:        189      20      24     197     230

  Fragments arranged by size: 

                 230     197     189      24      20
 
TatI w'GTAC_w

Cuts at:      0     327     483     660 
   Size:        327     156     177

  Fragments arranged by size: 

                 327     177     156
 
XmnI GAAnn'nnTTC

Cuts at:      0     392     508     660 
   Size:        392     116     152

  Fragments arranged by size: 

                 392     152     116
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AloI     ApoI     BbsI     BlpI     BsaI
    BseRI     BsgI    BsmBI    BspHI     BtgI     DrdI  Eco57MI    EcoRI
    Hin4I   HincII  HindIII     MmeI     NcoI     NdeI     NspI     PciI
    PflMI     PsrI     PstI     SfcI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI