(Linear) (Six-base) MAPSORT of: KIEE0192.seq  Check: 1270  from: 1  to: 261



KIEE0192.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

BanI G'GyrC_C

Cuts at:      0     250     261 
   Size:        250      11
 
Bme1580I G_kGCm'C

Cuts at:      0     255     261 
   Size:        255       6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     261 
   Size:          2     259
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73      76     235     246     261 
   Size:         73       3     159      11      15

  Fragments arranged by size: 

                 159      73      15      11       3
 
BseYI C'CCAG_C

Cuts at:      0     137     261 
   Size:        137     124
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     193     261 
   Size:        142      51      68

  Fragments arranged by size: 

                 142      68      51
 
Bsp1286I G_dGCh'C

Cuts at:      0     255     261 
   Size:        255       6
 
BsrBI CCG'CTC

Cuts at:      0      92     261 
   Size:         92     169
 
BsrDI GCAATG_nn'

Cuts at:      0     163     261 
   Size:        163      98
 
BsrFI r'CCGG_y

Cuts at:      0     247     261 
   Size:        247      14
 
BtsI GCAGTG_nn'

Cuts at:      0     125     261 
   Size:        125     136
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     261 
   Size:         66     195
 
HincII GTy'rAC

Cuts at:      0       5     261 
   Size:          5     256
 
MspA1I CmG'CkG

Cuts at:      0      72      81     261 
   Size:         72       9     180

  Fragments arranged by size: 

                 180      72       9
 
PpuMI rG'GwC_Cy

Cuts at:      0      66     261 
   Size:         66     195
 
PstI C_TGCA'G

Cuts at:      0     230     261 
   Size:        230      31
 
PvuII CAG'CTG

Cuts at:      0      81     261 
   Size:         81     180
 
SfcI C'TryA_G

Cuts at:      0     226     261 
   Size:        226      35
 
SmlI C'TyrA_G

Cuts at:      0      17     261 
   Size:         17     244
 

 Enzymes that do cut:

     BanI Bme1580I    BpuEI    BseRI    BseYI     BsgI Bsp1286I    BsrBI
    BsrDI    BsrFI     BtsI EcoO109I   HincII   MspA1I    PpuMI     PstI
    PvuII     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI