Restriction map for
pF1KE0193
(Linear) (Six-base) MAPSORT of: KIEE0193.seq Check: 4800 from: 1 to: 1077
KIEE0193.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 1077
Size: 324 753
AccI GT'mk_AC
Cuts at: 0 473 1077
Size: 473 604
AclI AA'CG_TT
Cuts at: 0 139 1077
Size: 139 938
AfeI AGC'GCT
Cuts at: 0 1069 1077
Size: 1069 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 766 1077
Size: 766 311
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 562 594 1077
Size: 562 32 483
Fragments arranged by size:
562 483 32
AlwNI CAG_nnn'CTG
Cuts at: 0 427 1077
Size: 427 650
ApaLI G'TGCA_C
Cuts at: 0 337 1077
Size: 337 740
ApoI r'AATT_y
Cuts at: 0 448 1077
Size: 448 629
AvaI C'yCGr_G
Cuts at: 0 59 1077
Size: 59 1018
BanI G'GyrC_C
Cuts at: 0 286 303 787 1077
Size: 286 17 484 290
Fragments arranged by size:
484 290 286 17
BbvCI CC'TCA_GC
Cuts at: 0 583 1077
Size: 583 494
BclI T'GATC_A
Cuts at: 0 591 1077
Size: 591 486
Bme1580I G_kGCm'C
Cuts at: 0 196 341 755 1077
Size: 196 145 414 322
Fragments arranged by size:
414 322 196 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 333 812 1077
Size: 240 93 479 265
Fragments arranged by size:
479 265 240 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1077
Size: 512 565
Bpu10I CC'TnA_GC
Cuts at: 0 583 1077
Size: 583 494
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 455 485 562 592 1077
Size: 455 30 77 30 485
Fragments arranged by size:
485 455 77 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 783 1077
Size: 505 278 294
Fragments arranged by size:
505 294 278
BseYI C'CCAG_C
Cuts at: 0 459 621 1077
Size: 459 162 456
Fragments arranged by size:
459 456 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 615 1077
Size: 615 462
BsiHKAI G_wGCw'C
Cuts at: 0 164 341 1077
Size: 164 177 736
Fragments arranged by size:
736 177 164
Bsp1286I G_dGCh'C
Cuts at: 0 164 196 341 755 1077
Size: 164 32 145 414 322
Fragments arranged by size:
414 322 164 145 32
BspHI T'CATG_A
Cuts at: 0 594 822 1077
Size: 594 228 255
Fragments arranged by size:
594 255 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 1077
Size: 324 753
BsrBI CCG'CTC
Cuts at: 0 600 1077
Size: 600 477
BsrDI GCAATG_nn'
Cuts at: 0 234 1077
Size: 234 843
BstYI r'GATC_y
Cuts at: 0 807 1077
Size: 807 270
BtgI C'CryG_G
Cuts at: 0 233 351 563 771 1077
Size: 233 118 212 208 306
Fragments arranged by size:
306 233 212 208 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 60 1077
Size: 60 1017
BtsI GCAGTG_nn'
Cuts at: 0 43 946 1077
Size: 43 903 131
Fragments arranged by size:
903 131 43
EarI CTCTTCn'nnn_
Cuts at: 0 508 869 979 1077
Size: 508 361 110 98
Fragments arranged by size:
508 361 110 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 751 1077
Size: 244 507 326
Fragments arranged by size:
507 326 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1077
Size: 512 565
EcoO109I rG'GnC_Cy
Cuts at: 0 16 424 1077
Size: 16 408 653
Fragments arranged by size:
653 408 16
HaeII r_GCGC'y
Cuts at: 0 1071 1077
Size: 1071 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 927 959 1077
Size: 927 32 118
Fragments arranged by size:
927 118 32
MslI CAynn'nnrTG
Cuts at: 0 238 380 748 893 1077
Size: 238 142 368 145 184
Fragments arranged by size:
368 238 184 145 142
MspA1I CmG'CkG
Cuts at: 0 540 773 1077
Size: 540 233 304
Fragments arranged by size:
540 304 233
NcoI C'CATG_G
Cuts at: 0 233 351 563 1077
Size: 233 118 212 514
Fragments arranged by size:
514 233 212 118
PflMI CCAn_nnn'nTGG
Cuts at: 0 189 1077
Size: 189 888
PfoI T'CCnGG_A
Cuts at: 0 70 528 1077
Size: 70 458 549
Fragments arranged by size:
549 458 70
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 562 594 1077
Size: 562 32 483
Fragments arranged by size:
562 483 32
PpuMI rG'GwC_Cy
Cuts at: 0 16 1077
Size: 16 1061
SacII CC_GC'GG
Cuts at: 0 774 1077
Size: 774 303
SanDI GG'GwC_CC
Cuts at: 0 16 1077
Size: 16 1061
ScaI AGT'ACT
Cuts at: 0 148 1077
Size: 148 929
SfcI C'TryA_G
Cuts at: 0 614 1077
Size: 614 463
StyI C'CwwG_G
Cuts at: 0 21 233 351 563 1077
Size: 21 212 118 212 514
Fragments arranged by size:
514 212 212 118 21
TatI w'GTAC_w
Cuts at: 0 146 1077
Size: 146 931
Enzymes that do cut:
AarI AccI AclI AfeI AlfI AloI AlwNI ApaLI
ApoI AvaI BanI BbvCI BclI Bme1580I BmrI BpmI
Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BstYI BtgI BtgZI BtsI EarI
EciI Eco57MI EcoO109I HaeII Hin4I MslI MspA1I NcoI
PflMI PfoI PpiI PpuMI SacII SanDI ScaI SfcI
StyI TatI
Enzymes that do not cut:
AatII Acc65I AcuI AflII AflIII AgeI AhdI AleI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII
BbeI BbsI BcgI BciVI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI