(Linear) (Six-base) MAPSORT of: KIEE0197.seq Check: 3859 from: 1 to: 357 KIEE0197.orf With 182 enzymes: SgfI * July 15, 2008 09:38 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 208 357 Size: 154 54 149 Fragments arranged by size: 154 149 54 ApoI r'AATT_y Cuts at: 0 143 357 Size: 143 214 BbvCI CC'TCA_GC Cuts at: 0 52 357 Size: 52 305 Bme1580I G_kGCm'C Cuts at: 0 168 357 Size: 168 189 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 357 Size: 268 89 Bpu10I CC'TnA_GC Cuts at: 0 52 76 357 Size: 52 24 281 Fragments arranged by size: 281 52 24 Bsp1286I G_dGCh'C Cuts at: 0 168 357 Size: 168 189 BssSI C'ACGA_G Cuts at: 0 31 357 Size: 31 326 BstBI TT'CG_AA Cuts at: 0 104 357 Size: 104 253 BstXI CCAn_nnnn'nTGG Cuts at: 0 15 357 Size: 15 342 DraI TTT'AAA Cuts at: 0 260 357 Size: 260 97 EarI CTCTTCn'nnn_ Cuts at: 0 322 357 Size: 322 35 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 208 268 357 Size: 154 54 60 89 Fragments arranged by size: 154 89 60 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 113 145 357 Size: 113 32 212 Fragments arranged by size: 212 113 32 HindIII A'AGCT_T Cuts at: 0 254 357 Size: 254 103 SfcI C'TryA_G Cuts at: 0 81 357 Size: 81 276 StyI C'CwwG_G Cuts at: 0 8 357 Size: 8 349 Enzymes that do cut: AcuI ApoI BbvCI Bme1580I BpmI Bpu10I Bsp1286I BssSI BstBI BstXI DraI EarI Eco57MI Hin4I HindIII SfcI StyI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI