Restriction map for
pF1KE0199
(Linear) (Six-base) MAPSORT of: KIEE0199.seq Check: 333 from: 1 to: 3318
KIEE0199.orf
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 971 3318
Size: 971 2347
AccI GT'mk_AC
Cuts at: 0 2051 3318
Size: 2051 1267
Acc65I G'GTAC_C
Cuts at: 0 1576 3318
Size: 1576 1742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 973 1008 1445 1766 2225 2606 3318
Size: 556 417 35 437 321 459 381 712
Fragments arranged by size:
712 556 459 437 417 381 321 35
AflIII A'CryG_T
Cuts at: 0 992 1143 3318
Size: 992 151 2175
Fragments arranged by size:
2175 992 151
AgeI A'CCGG_T
Cuts at: 0 1573 3318
Size: 1573 1745
AhdI GACnn_n'nnGTC
Cuts at: 0 1544 3318
Size: 1544 1774
AleI CACnn'nnGTG
Cuts at: 0 903 977 2510 3318
Size: 903 74 1533 808
Fragments arranged by size:
1533 903 808 74
AlwNI CAG_nnn'CTG
Cuts at: 0 2218 3174 3318
Size: 2218 956 144
Fragments arranged by size:
2218 956 144
ApaI G_GGCC'C
Cuts at: 0 81 196 434 1332 1603 2522 3239 3318
Size: 81 115 238 898 271 919 717 79
Fragments arranged by size:
919 898 717 271 238 115 81 79
ApaLI G'TGCA_C
Cuts at: 0 757 906 3318
Size: 757 149 2412
Fragments arranged by size:
2412 757 149
ApoI r'AATT_y
Cuts at: 0 733 2916 3298 3318
Size: 733 2183 382 20
Fragments arranged by size:
2183 733 382 20
AvaI C'yCGr_G
Cuts at: 0 479 509 661 1211 1541 3318
Size: 479 30 152 550 330 1777
Fragments arranged by size:
1777 550 479 330 152 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1560 1568 1593 1601 3318
Size: 1560 8 25 8 1717
Fragments arranged by size:
1717 1560 25 8 8
BamHI G'GATC_C
Cuts at: 0 624 3318
Size: 624 2694
BanI G'GyrC_C
Cuts at: 0 379 920 1187 1215 1300 1576 2456 2487
Size: 379 541 267 28 85 276 880 31
Cuts at: 2487 3318
Size: 831
Fragments arranged by size:
880 831 541 379 276 267 85 31
28
BanII G_rGCy'C
Cuts at: 0 47 81 196 391 434 1241 1332 1603
Size: 47 34 115 195 43 807 91 271
Cuts at: 1603 2522 3167 3239 3318
Size: 919 645 72 79
Fragments arranged by size:
919 807 645 271 195 115 91 79
72 47 43 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 961 1433 1834 3318
Size: 961 472 401 1484
Fragments arranged by size:
1484 961 472 401
BbvCI CC'TCA_GC
Cuts at: 0 835 3318
Size: 835 2483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1039 1073 3318
Size: 1039 34 2245
Fragments arranged by size:
2245 1039 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1876 1886 3318
Size: 1876 10 1432
Fragments arranged by size:
1876 1432 10
BclI T'GATC_A
Cuts at: 0 3195 3318
Size: 3195 123
BglI GCCn_nnn'nGGC
Cuts at: 0 1598 2259 3171 3318
Size: 1598 661 912 147
Fragments arranged by size:
1598 912 661 147
BlpI GC'TnA_GC
Cuts at: 0 2219 2684 3318
Size: 2219 465 634
Fragments arranged by size:
2219 634 465
Bme1580I G_kGCm'C
Cuts at: 0 81 196 246 434 761 910 1218 1332
Size: 81 115 50 188 327 149 308 114
Cuts at: 1332 1603 2461 2522 3239 3318
Size: 271 858 61 717 79
Fragments arranged by size:
858 717 327 308 271 188 149 115
114 81 79 61 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 62 77 822 846 1326 1600 2455 2504
Size: 62 15 745 24 480 274 855 49
Cuts at: 2504 3318
Size: 814
Fragments arranged by size:
855 814 745 480 274 62 49 24
15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 410 3200 3318
Size: 410 2790 118
Fragments arranged by size:
2790 410 118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 810 1419 1620 1934 2563 2813 3318
Size: 332 478 609 201 314 629 250 505
Fragments arranged by size:
629 609 505 478 332 314 250 201
Bpu10I CC'TnA_GC
Cuts at: 0 835 3318
Size: 835 2483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1513 2662 3318
Size: 1513 1149 656
Fragments arranged by size:
1513 1149 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 1552 3318
Size: 166 1386 1766
Fragments arranged by size:
1766 1386 166
BsaBI GATnn'nnATC
Cuts at: 0 342 3318
Size: 342 2976
BsaHI Gr'CG_yC
Cuts at: 0 1169 3318
Size: 1169 2149
BsaWI w'CCGG_w
Cuts at: 0 1573 3318
Size: 1573 1745
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 1508 1538 1902 1932 2710 2740
Size: 790 30 688 30 364 30 778 30
Cuts at: 2740 3210 3240 3318
Size: 470 30 78
Fragments arranged by size:
790 778 688 470 364 78 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1665 2555 3071 3173 3318
Size: 685 980 890 516 102 145
Fragments arranged by size:
980 890 685 516 145 102
BseYI C'CCAG_C
Cuts at: 0 307 405 1558 1751 1923 2068 2300 2555
Size: 307 98 1153 193 172 145 232 255
Cuts at: 2555 2597 3025 3318
Size: 42 428 293
Fragments arranged by size:
1153 428 307 293 255 232 193 172
145 98 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 239 1089 1391 3318
Size: 168 71 850 302 1927
Fragments arranged by size:
1927 850 302 168 71
BsiHKAI G_wGCw'C
Cuts at: 0 761 779 910 1004 3318
Size: 761 18 131 94 2314
Fragments arranged by size:
2314 761 131 94 18
BsmI GAATG_Cn'
Cuts at: 0 2088 3318
Size: 2088 1230
Bsp1286I G_dGCh'C
Cuts at: 0 47 81 196 246 391 434 761 779
Size: 47 34 115 50 145 43 327 18
Cuts at: 779 910 1004 1218 1241 1332 1603 2461 2522
Size: 131 94 214 23 91 271 858 61
Cuts at: 2522 3167 3239 3318
Size: 645 72 79
Fragments arranged by size:
858 645 327 271 214 145 131 115
94 91 79 72 61 50 47 43
34 23 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 971 1769 3318
Size: 290 681 798 1549
Fragments arranged by size:
1549 798 681 290
BsrBI CCG'CTC
Cuts at: 0 792 1251 1673 3318
Size: 792 459 422 1645
Fragments arranged by size:
1645 792 459 422
BsrDI GCAATG_nn'
Cuts at: 0 1035 1413 1457 2980 3318
Size: 1035 378 44 1523 338
Fragments arranged by size:
1523 1035 378 338 44
BsrFI r'CCGG_y
Cuts at: 0 1573 3318
Size: 1573 1745
BssSI C'ACGA_G
Cuts at: 0 1003 3318
Size: 1003 2315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 756 1369 1761 1777 3318
Size: 756 613 392 16 1541
Fragments arranged by size:
1541 756 613 392 16
BstEII G'GTnAC_C
Cuts at: 0 1902 3318
Size: 1902 1416
BstXI CCAn_nnnn'nTGG
Cuts at: 0 157 460 2484 2790 3318
Size: 157 303 2024 306 528
Fragments arranged by size:
2024 528 306 303 157
BstYI r'GATC_y
Cuts at: 0 624 1122 3277 3318
Size: 624 498 2155 41
Fragments arranged by size:
2155 624 498 41
Bsu36I CC'TnA_GG
Cuts at: 0 1501 3041 3318
Size: 1501 1540 277
Fragments arranged by size:
1540 1501 277
BtgI C'CryG_G
Cuts at: 0 783 1191 2090 2135 3318
Size: 783 408 899 45 1183
Fragments arranged by size:
1183 899 783 408 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 351 3318
Size: 351 2967
BtsI GCAGTG_nn'
Cuts at: 0 1353 1808 2151 3318
Size: 1353 455 343 1167
Fragments arranged by size:
1353 1167 455 343
EaeI y'GGCC_r
Cuts at: 0 1528 3318
Size: 1528 1790
EarI CTCTTCn'nnn_
Cuts at: 0 1487 2108 3318
Size: 1487 621 1210
Fragments arranged by size:
1487 1210 621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 556 810 973 1008 1419 1445 1620
Size: 332 224 254 163 35 411 26 175
Cuts at: 1620 1766 1934 2225 2563 2606 2813 3318
Size: 146 168 291 338 43 207 505
Fragments arranged by size:
505 411 338 332 291 254 224 207
175 168 163 146 43 35 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3086 3318
Size: 3086 232
EcoO109I rG'GnC_Cy
Cuts at: 0 77 78 192 430 431 514 657 839
Size: 77 1 114 238 1 83 143 182
Cuts at: 839 1261 1328 1450 1538 1600 2129 2200 2374
Size: 422 67 122 88 62 529 71 174
Cuts at: 2374 2518 2519 2587 3064 3236 3318
Size: 144 1 68 477 172 82
Fragments arranged by size:
529 477 422 238 182 174 172 144
143 122 114 88 83 82 77 71
68 67 62 1 1 1
EcoRI G'AATT_C
Cuts at: 0 3298 3318
Size: 3298 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1796 3318
Size: 1796 1522
FspI TGC'GCA
Cuts at: 0 877 3318
Size: 877 2441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 483 515 673 705 1114 1146 1250 1282
Size: 483 32 158 32 409 32 104 32
Cuts at: 1282 1530 1562 1824 1856 3318
Size: 248 32 262 32 1462
Fragments arranged by size:
1462 483 409 262 248 158 104 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 230 3318
Size: 230 3088
KpnI G_GTAC'C
Cuts at: 0 1580 3318
Size: 1580 1738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 3318
Size: 245 3073
MslI CAynn'nnrTG
Cuts at: 0 155 862 903 977 993 1070 2510 3250
Size: 155 707 41 74 16 77 1440 740
Cuts at: 3250 3318
Size: 68
Fragments arranged by size:
1440 740 707 155 77 74 68 41
16
MspA1I CmG'CkG
Cuts at: 0 182 534 753 2218 3029 3138 3201 3318
Size: 182 352 219 1465 811 109 63 117
Fragments arranged by size:
1465 811 352 219 182 117 109 63
NcoI C'CATG_G
Cuts at: 0 2090 2135 3318
Size: 2090 45 1183
Fragments arranged by size:
2090 1183 45
NdeI CA'TA_TG
Cuts at: 0 2841 3318
Size: 2841 477
NruI TCG'CGA
Cuts at: 0 336 3318
Size: 336 2982
NspI r_CATG'y
Cuts at: 0 555 861 876 996 1147 3318
Size: 555 306 15 120 151 2171
Fragments arranged by size:
2171 555 306 151 120 15
PasI CC'CwG_GG
Cuts at: 0 1324 3168 3318
Size: 1324 1844 150
Fragments arranged by size:
1844 1324 150
PciI A'CATG_T
Cuts at: 0 992 1143 3318
Size: 992 151 2175
Fragments arranged by size:
2175 992 151
PflMI CCAn_nnn'nTGG
Cuts at: 0 1324 1717 2125 3318
Size: 1324 393 408 1193
Fragments arranged by size:
1324 1193 408 393
PfoI T'CCnGG_A
Cuts at: 0 348 1878 3318
Size: 348 1530 1440
Fragments arranged by size:
1530 1440 348
PpuMI rG'GwC_Cy
Cuts at: 0 514 657 1261 1450 1538 2129 2200 3064
Size: 514 143 604 189 88 591 71 864
Cuts at: 3064 3318
Size: 254
Fragments arranged by size:
864 604 591 514 254 189 143 88
71
PspOMI G'GGCC_C
Cuts at: 0 77 192 430 1328 1599 2518 3235 3318
Size: 77 115 238 898 271 919 717 83
Fragments arranged by size:
919 898 717 271 238 115 83 77
PstI C_TGCA'G
Cuts at: 0 287 727 1015 1345 1815 3318
Size: 287 440 288 330 470 1503
Fragments arranged by size:
1503 470 440 330 288 287
PvuII CAG'CTG
Cuts at: 0 182 534 753 2218 3029 3138 3318
Size: 182 352 219 1465 811 109 180
Fragments arranged by size:
1465 811 352 219 182 180 109
SanDI GG'GwC_CC
Cuts at: 0 514 1538 3318
Size: 514 1024 1780
Fragments arranged by size:
1780 1024 514
SapI GCTCTTCn'nnn_
Cuts at: 0 2108 3318
Size: 2108 1210
SfcI C'TryA_G
Cuts at: 0 283 723 1011 1229 1341 1811 2107 2397
Size: 283 440 288 218 112 470 296 290
Cuts at: 2397 3318
Size: 921
Fragments arranged by size:
921 470 440 296 290 288 283 218
112
SmaI CCC'GGG
Cuts at: 0 481 511 1543 3318
Size: 481 30 1032 1775
Fragments arranged by size:
1775 1032 481 30
SmlI C'TyrA_G
Cuts at: 0 661 1492 2677 3318
Size: 661 831 1185 641
Fragments arranged by size:
1185 831 661 641
StuI AGG'CCT
Cuts at: 0 2308 2634 3318
Size: 2308 326 684
Fragments arranged by size:
2308 684 326
StyI C'CwwG_G
Cuts at: 0 581 1855 2090 2135 2378 3318
Size: 581 1274 235 45 243 940
Fragments arranged by size:
1274 940 581 243 235 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 1055 2013 2286 2341 2674 3318
Size: 321 734 958 273 55 333 644
Fragments arranged by size:
958 734 644 333 321 273 55
TatI w'GTAC_w
Cuts at: 0 854 3318
Size: 854 2464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 790 822 1902 1934 2710 2742 3318
Size: 790 32 1080 32 776 32 576
Fragments arranged by size:
1080 790 776 576 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1230 2637 3249 3318
Size: 1230 1407 612 69
Fragments arranged by size:
1407 1230 612 69
XhoI C'TCGA_G
Cuts at: 0 661 3318
Size: 661 2657
XmaI C'CCGG_G
Cuts at: 0 479 509 1541 3318
Size: 479 30 1032 1777
Fragments arranged by size:
1777 1032 479 30
XmnI GAAnn'nnTTC
Cuts at: 0 799 3318
Size: 799 2519
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I HincII
KpnI MmeI MslI MspA1I NcoI NdeI NruI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SanDI SapI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AlfI AloI AscI AseI
AsiSI AvrII BbeI BfrBI BglII BmgBI BmtI BsaAI
BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspAI HaeII HindIII HpaI KasI
MfeI MluI MscI NaeI NarI NgoMIV NheI NotI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI ZraI