(Linear) (Six-base) MAPSORT of: KIEE0201.seq  Check: 8472  from: 1  to: 249



KIEE0201.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AatII G_ACGT'C

Cuts at:      0      89     249 
   Size:         89     160
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     249 
   Size:        130     119
 
AvaI C'yCGr_G

Cuts at:      0     143     249 
   Size:        143     106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     151     249 
   Size:         75      76      98

  Fragments arranged by size: 

                  98      76      75
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     249 
   Size:        220      29
 
BsaHI Gr'CG_yC

Cuts at:      0      86     249 
   Size:         86     163
 
BseYI C'CCAG_C

Cuts at:      0      60      66     249 
   Size:         60       6     183

  Fragments arranged by size: 

                 183      60       6
 
BsiEI CG_ry'CG

Cuts at:      0      47     249 
   Size:         47     202
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      93     249 
   Size:         93     156
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      99     249 
   Size:         99     150
 
EarI CTCTTCn'nnn_

Cuts at:      0      13     249 
   Size:         13     236
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     130     151     249 
   Size:         75      55      21      98

  Fragments arranged by size: 

                  98      75      55      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0      77     249 
   Size:         77     172
 
PasI CC'CwG_GG

Cuts at:      0      72     249 
   Size:         72     177
 
PpuMI rG'GwC_Cy

Cuts at:      0      77     249 
   Size:         77     172
 
PvuI CG_AT'CG

Cuts at:      0      47     249 
   Size:         47     202
 
SanDI GG'GwC_CC

Cuts at:      0      77     249 
   Size:         77     172
 
SapI GCTCTTCn'nnn_

Cuts at:      0      13     249 
   Size:         13     236
 
SmlI C'TyrA_G

Cuts at:      0     235     249 
   Size:        235      14
 
StyI C'CwwG_G

Cuts at:      0      98     249 
   Size:         98     151
 
ZraI GAC'GTC

Cuts at:      0      87     249 
   Size:         87     162
 

 Enzymes that do cut:

    AatII     AcuI     AvaI     BpmI    BpuEI    BsaHI    BseYI    BsiEI
    BsmBI    BstXI     EarI  Eco57MI EcoO109I     PasI    PpuMI     PvuI
    SanDI     SapI     SmlI     StyI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI    PvuII    RsrII     SacI    SacII     SalI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI