(Linear) (Six-base) MAPSORT of: KIEE0202.seq  Check: 5786  from: 1  to: 804



KIEE0202.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     494     797     804 
   Size:        494     303       7

  Fragments arranged by size: 

                 494     303       7
 
ApoI r'AATT_y

Cuts at:      0     133     339     674     804 
   Size:        133     206     335     130

  Fragments arranged by size: 

                 335     206     133     130
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     739     785     804 
   Size:        739      46      19

  Fragments arranged by size: 

                 739      46      19
 
BclI T'GATC_A

Cuts at:      0     444     804 
   Size:        444     360
 
BglII A'GATC_T

Cuts at:      0     303     804 
   Size:        303     501
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     251     804 
   Size:         46     205     553

  Fragments arranged by size: 

                 553     205      46
 
BsaWI w'CCGG_w

Cuts at:      0     540     804 
   Size:        540     264
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     493     574     577     592     804 
   Size:         64     429      81       3      15     212

  Fragments arranged by size: 

                 429     212      81      64      15       3
 
BseYI C'CCAG_C

Cuts at:      0     181     804 
   Size:        181     623
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     604     804 
   Size:        604     200
 
BstBI TT'CG_AA

Cuts at:      0     198     804 
   Size:        198     606
 
BstYI r'GATC_y

Cuts at:      0      40     303     804 
   Size:         40     263     501

  Fragments arranged by size: 

                 501     263      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     512     804 
   Size:        512     292
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     267     302     804 
   Size:        267      35     502

  Fragments arranged by size: 

                 502     267      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     470     506     512     551     563     569     632     716 
   Size:        470      36       6      39      12       6      63      84

Cuts at:    716     725     754     804 
   Size:          9      29      50

  Fragments arranged by size: 

                 470      84      63      50      39      36      29      12
                   9       6       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     251     494     797     804 
   Size:         46     205     243     303       7

  Fragments arranged by size: 

                 303     243     205      46       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     763     804 
   Size:        763      41
 
HaeII r_GCGC'y

Cuts at:      0     466     804 
   Size:        466     338
 
PstI C_TGCA'G

Cuts at:      0     419     804 
   Size:        419     385
 
SfcI C'TryA_G

Cuts at:      0     415     804 
   Size:        415     389
 
StuI AGG'CCT

Cuts at:      0     107     804 
   Size:        107     697
 
TatI w'GTAC_w

Cuts at:      0     310     804 
   Size:        310     494
 
XmnI GAAnn'nnTTC

Cuts at:      0     674     804 
   Size:        674     130
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI     BclI    BglII     BpmI    BsaWI    BseRI
    BseYI    BspMI    BstBI    BstYI    BtgZI    CspCI     EarI  Eco57MI
     FalI    HaeII     PstI     SfcI     StuI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI