(Linear) (Six-base) MAPSORT of: KIEE0210.seq  Check: 5573  from: 1  to: 810



KIEE0210.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AfeI AGC'GCT

Cuts at:      0     296     810 
   Size:        296     514
 
AflII C'TTAA_G

Cuts at:      0     637     810 
   Size:        637     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     100     121     124     810 
   Size:        100      21       3     686

  Fragments arranged by size: 

                 686     100      21       3
 
ApoI r'AATT_y

Cuts at:      0     203     220     409     810 
   Size:        203      17     189     401

  Fragments arranged by size: 

                 401     203     189      17
 
BbvCI CC'TCA_GC

Cuts at:      0     307     810 
   Size:        307     503
 
BfrBI ATG'CAT

Cuts at:      0     198     810 
   Size:        198     612
 
BglII A'GATC_T

Cuts at:      0     667     810 
   Size:        667     143
 
Bme1580I G_kGCm'C

Cuts at:      0     343     810 
   Size:        343     467
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     810 
   Size:        150     660
 
Bpu10I CC'TnA_GC

Cuts at:      0     307     810 
   Size:        307     503
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     810 
   Size:        237     573
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     377     810 
   Size:        377     433
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     361     810 
   Size:        361     449
 
Bsp1286I G_dGCh'C

Cuts at:      0     343     810 
   Size:        343     467
 
BspHI T'CATG_A

Cuts at:      0     176     810 
   Size:        176     634
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     121     195     224     810 
   Size:        121      74      29     586

  Fragments arranged by size: 

                 586     121      74      29
 
BstYI r'GATC_y

Cuts at:      0     466     667     810 
   Size:        466     201     143

  Fragments arranged by size: 

                 466     201     143
 
BtgI C'CryG_G

Cuts at:      0     314     810 
   Size:        314     496
 
DraI TTT'AAA

Cuts at:      0     108     810 
   Size:        108     702
 
EaeI y'GGCC_r

Cuts at:      0     317     810 
   Size:        317     493
 
EarI CTCTTCn'nnn_

Cuts at:      0     329     729     810 
   Size:        329     400      81

  Fragments arranged by size: 

                 400     329      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     810 
   Size:        150     660
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     663     810 
   Size:        663     147
 
HaeII r_GCGC'y

Cuts at:      0     298     810 
   Size:        298     512
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     789     810 
   Size:        789      21
 
HincII GTy'rAC

Cuts at:      0     421     810 
   Size:        421     389
 
MscI TGG'CCA

Cuts at:      0     319     810 
   Size:        319     491
 
MslI CAynn'nnrTG

Cuts at:      0     788     810 
   Size:        788      22
 
MspA1I CmG'CkG

Cuts at:      0     121     551     810 
   Size:        121     430     259

  Fragments arranged by size: 

                 430     259     121
 
NcoI C'CATG_G

Cuts at:      0     314     810 
   Size:        314     496
 
NsiI A_TGCA'T

Cuts at:      0     200     810 
   Size:        200     610
 
PfoI T'CCnGG_A

Cuts at:      0     558     810 
   Size:        558     252
 
PshAI GACnn'nnGTC

Cuts at:      0      22     810 
   Size:         22     788
 
PvuII CAG'CTG

Cuts at:      0     121     810 
   Size:        121     689
 
SexAI A'CCwGG_T

Cuts at:      0     377     810 
   Size:        377     433
 
SfcI C'TryA_G

Cuts at:      0     775     810 
   Size:        775      35
 
SmlI C'TyrA_G

Cuts at:      0     252     637     810 
   Size:        252     385     173

  Fragments arranged by size: 

                 385     252     173
 
StyI C'CwwG_G

Cuts at:      0      66     314     462     810 
   Size:         66     248     148     348

  Fragments arranged by size: 

                 348     248     148      66
 

 Enzymes that do cut:

     AfeI    AflII    AlwNI     ApoI    BbvCI    BfrBI    BglII Bme1580I
     BpmI   Bpu10I    BpuEI    BseRI    BsmBI Bsp1286I    BspHI   BstAPI
    BstYI     BtgI     DraI     EaeI     EarI  Eco57MI     FalI    HaeII
    Hin4I   HincII     MscI     MslI   MspA1I     NcoI     NsiI     PfoI
    PshAI    PvuII    SexAI     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI     BlpI    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI