(Linear) (Six-base) MAPSORT of: KIEE0211.seq  Check: 316  from: 1  to: 258



KIEE0211.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      47     258 
   Size:         47     211
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     258 
   Size:        202      56
 
AvaI C'yCGr_G

Cuts at:      0     247     258 
   Size:        247      11
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     258 
   Size:         52     206
 
BsiHKAI G_wGCw'C

Cuts at:      0      38     258 
   Size:         38     220
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     258 
   Size:         38     220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      47     258 
   Size:         47     211
 
BsrDI GCAATG_nn'

Cuts at:      0     161     258 
   Size:        161      97
 
DraIII CAC_nnn'GTG

Cuts at:      0      83     258 
   Size:         83     175
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     202     258 
   Size:         52     150      56

  Fragments arranged by size: 

                 150      56      52
 
MspA1I CmG'CkG

Cuts at:      0      30     258 
   Size:         30     228
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      83     258 
   Size:         83     175
 
PstI C_TGCA'G

Cuts at:      0      44     258 
   Size:         44     214
 
PvuII CAG'CTG

Cuts at:      0      30     258 
   Size:         30     228
 
SfcI C'TryA_G

Cuts at:      0      40     258 
   Size:         40     218
 
TatI w'GTAC_w

Cuts at:      0      50     258 
   Size:         50     208
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28      60     258 
   Size:         28      32     198

  Fragments arranged by size: 

                 198      32      28
 

 Enzymes that do cut:

     AarI     AcuI     AvaI     BpmI  BsiHKAI Bsp1286I    BspMI    BsrDI
   DraIII  Eco57MI   MspA1I    PflMI     PstI    PvuII     SfcI     TatI
     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI