(Linear) (Six-base) MAPSORT of: KIEE0212.seq  Check: 525  from: 1  to: 183



KIEE0212.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AatII G_ACGT'C

Cuts at:      0     160     183 
   Size:        160      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     176     183 
   Size:        176       7
 
BglI GCCn_nnn'nGGC

Cuts at:      0     116     183 
   Size:        116      67
 
Bme1580I G_kGCm'C

Cuts at:      0     135     183 
   Size:        135      48
 
BsaHI Gr'CG_yC

Cuts at:      0     157     183 
   Size:        157      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     135     183 
   Size:        135      48
 
BsrFI r'CCGG_y

Cuts at:      0      46     183 
   Size:         46     137
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68      83     183 
   Size:         68      15     100

  Fragments arranged by size: 

                 100      68      15
 
EarI CTCTTCn'nnn_

Cuts at:      0      85     183 
   Size:         85      98
 
MspA1I CmG'CkG

Cuts at:      0     173     183 
   Size:        173      10
 
NaeI GCC'GGC

Cuts at:      0      48     183 
   Size:         48     135
 
NgoMIV G'CCGG_C

Cuts at:      0      46     183 
   Size:         46     137
 
PasI CC'CwG_GG

Cuts at:      0     135     183 
   Size:        135      48
 
PvuII CAG'CTG

Cuts at:      0     173     183 
   Size:        173      10
 
SapI GCTCTTCn'nnn_

Cuts at:      0      85     183 
   Size:         85      98
 
ZraI GAC'GTC

Cuts at:      0     158     183 
   Size:        158      25
 

 Enzymes that do cut:

    AatII    AlwNI     BglI Bme1580I    BsaHI Bsp1286I    BsrFI   BstAPI
     EarI   MspA1I     NaeI   NgoMIV     PasI    PvuII     SapI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI