(Linear) (Six-base) MAPSORT of: KIEE0213.seq  Check: 3948  from: 1  to: 960



KIEE0213.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     738     960 
   Size:        738     222
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     419     454     960 
   Size:        377      42      35     506

  Fragments arranged by size: 

                 506     377      42      35
 
ApoI r'AATT_y

Cuts at:      0     226     870     960 
   Size:        226     644      90

  Fragments arranged by size: 

                 644     226      90
 
AseI AT'TA_AT

Cuts at:      0     238     960 
   Size:        238     722
 
BclI T'GATC_A

Cuts at:      0     795     960 
   Size:        795     165
 
BlpI GC'TnA_GC

Cuts at:      0     143     960 
   Size:        143     817
 
Bme1580I G_kGCm'C

Cuts at:      0      97     960 
   Size:         97     863
 
BmgBI CAC'GTC

Cuts at:      0     420     960 
   Size:        420     540
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     566     960 
   Size:        566     394
 
Bpu10I CC'TnA_GC

Cuts at:      0     527     960 
   Size:        527     433
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     494     960 
   Size:        387     107     466

  Fragments arranged by size: 

                 466     387     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     476     751     960 
   Size:        476     275     209

  Fragments arranged by size: 

                 476     275     209
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     960 
   Size:        118     842
 
BseYI C'CCAG_C

Cuts at:      0     784     960 
   Size:        784     176
 
Bsp1286I G_dGCh'C

Cuts at:      0      97     960 
   Size:         97     863
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     750     960 
   Size:        750     210
 
BsrBI CCG'CTC

Cuts at:      0      51     960 
   Size:         51     909
 
BstBI TT'CG_AA

Cuts at:      0     732     960 
   Size:        732     228
 
BstYI r'GATC_y

Cuts at:      0     189     960 
   Size:        189     771
 
BstZ17I GTA'TAC

Cuts at:      0     739     960 
   Size:        739     221
 
DraIII CAC_nnn'GTG

Cuts at:      0     604     960 
   Size:        604     356
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     938     960 
   Size:         74     864      22

  Fragments arranged by size: 

                 864      74      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     419     454     566     960 
   Size:        377      42      35     112     394

  Fragments arranged by size: 

                 394     377     112      42      35
 
HaeII r_GCGC'y

Cuts at:      0     150     960 
   Size:        150     810
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     780     812     960 
   Size:        193      32     555      32     148

  Fragments arranged by size: 

                 555     193     148      32      32
 
HincII GTy'rAC

Cuts at:      0     125     960 
   Size:        125     835
 
MspA1I CmG'CkG

Cuts at:      0     450     960 
   Size:        450     510
 
NspI r_CATG'y

Cuts at:      0     295     960 
   Size:        295     665
 
PfoI T'CCnGG_A

Cuts at:      0     823     960 
   Size:        823     137
 
PstI C_TGCA'G

Cuts at:      0     165     960 
   Size:        165     795
 
SapI GCTCTTCn'nnn_

Cuts at:      0     938     960 
   Size:        938      22
 
ScaI AGT'ACT

Cuts at:      0     326     960 
   Size:        326     634
 
SfcI C'TryA_G

Cuts at:      0     161     960 
   Size:        161     799
 
SmlI C'TyrA_G

Cuts at:      0     402     509     960 
   Size:        402     107     451

  Fragments arranged by size: 

                 451     402     107
 
TatI w'GTAC_w

Cuts at:      0     324     635     960 
   Size:        324     311     325

  Fragments arranged by size: 

                 325     324     311
 
XmnI GAAnn'nnTTC

Cuts at:      0      68     960 
   Size:         68     892
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AseI     BclI     BlpI Bme1580I    BmgBI
     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI Bsp1286I    BspMI
    BsrBI    BstBI    BstYI  BstZ17I   DraIII     EarI  Eco57MI    HaeII
    Hin4I   HincII   MspA1I     NspI     PfoI     PstI     SapI     ScaI
     SfcI     SmlI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BmrI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI