(Linear) (Six-base) MAPSORT of: KIEE0215.seq  Check: 1752  from: 1  to: 333



KIEE0215.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     323     333 
   Size:        323      10
 
Acc65I G'GTAC_C

Cuts at:      0     230     333 
   Size:        230     103
 
AfeI AGC'GCT

Cuts at:      0     280     333 
   Size:        280      53
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     222     255     333 
   Size:        222      33      78

  Fragments arranged by size: 

                 222      78      33
 
BanI G'GyrC_C

Cuts at:      0     230     333 
   Size:        230     103
 
BmgBI CAC'GTC

Cuts at:      0     150     333 
   Size:        150     183
 
BsaBI GATnn'nnATC

Cuts at:      0     202     333 
   Size:        202     131
 
BsaWI w'CCGG_w

Cuts at:      0      27     333 
   Size:         27     306
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205     333 
   Size:        205     128
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     333 
   Size:        238      95
 
BsiEI CG_ry'CG

Cuts at:      0      53     333 
   Size:         53     280
 
BsiHKAI G_wGCw'C

Cuts at:      0     118     248     274     333 
   Size:        118     130      26      59

  Fragments arranged by size: 

                 130     118      59      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     248     274     333 
   Size:        118     130      26      59

  Fragments arranged by size: 

                 130     118      59      26
 
BsrGI T'GTAC_A

Cuts at:      0      80     333 
   Size:         80     253
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     252     333 
   Size:        252      81
 
BstYI r'GATC_y

Cuts at:      0     134     333 
   Size:        134     199
 
BtsI GCAGTG_nn'

Cuts at:      0     183     246     333 
   Size:        183      63      87

  Fragments arranged by size: 

                 183      87      63
 
EaeI y'GGCC_r

Cuts at:      0      50     333 
   Size:         50     283
 
EagI C'GGCC_G

Cuts at:      0      50     333 
   Size:         50     283
 
EcoO109I rG'GnC_Cy

Cuts at:      0     127     333 
   Size:        127     206
 
HaeII r_GCGC'y

Cuts at:      0      79     282     333 
   Size:         79     203      51

  Fragments arranged by size: 

                 203      79      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     333 
   Size:        119      32     182

  Fragments arranged by size: 

                 182     119      32
 
KpnI G_GTAC'C

Cuts at:      0     234     333 
   Size:        234      99
 
NotI GC'GGCC_GC

Cuts at:      0      50     333 
   Size:         50     283
 
PfoI T'CCnGG_A

Cuts at:      0     118     333 
   Size:        118     215
 
PpuMI rG'GwC_Cy

Cuts at:      0     127     333 
   Size:        127     206
 
PstI C_TGCA'G

Cuts at:      0     269     333 
   Size:        269      64
 
SanDI GG'GwC_CC

Cuts at:      0     127     333 
   Size:        127     206
 
SfcI C'TryA_G

Cuts at:      0     141     265     333 
   Size:        141     124      68

  Fragments arranged by size: 

                 141     124      68
 
TatI w'GTAC_w

Cuts at:      0      80     333 
   Size:         80     253
 

 Enzymes that do cut:

     AccI   Acc65I     AfeI     BaeI     BanI    BmgBI    BsaBI    BsaWI
    BseRI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrGI   BstAPI    BstYI
     BtsI     EaeI     EagI EcoO109I    HaeII    Hin4I     KpnI     NotI
     PfoI    PpuMI     PstI    SanDI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseYI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI